Preprint Article Version 1 This version is not peer-reviewed

Qualitative Phytochemical Screening and Antioxidant Activities from Three Different Citrus Leaves (Rutaceae)

Version 1 : Received: 27 August 2019 / Approved: 28 August 2019 / Online: 28 August 2019 (11:16:06 CEST)

How to cite: Z., N.I..; Y., N.; K., S..; A.W., N.H.. Qualitative Phytochemical Screening and Antioxidant Activities from Three Different Citrus Leaves (Rutaceae). Preprints 2019, 2019080293 (doi: 10.20944/preprints201908.0293.v1). Z., N.I..; Y., N.; K., S..; A.W., N.H.. Qualitative Phytochemical Screening and Antioxidant Activities from Three Different Citrus Leaves (Rutaceae). Preprints 2019, 2019080293 (doi: 10.20944/preprints201908.0293.v1).

Abstract

Leaves of Citrus aurantifolia, Citrus hystrix and Citrus microcarpa collected from Mersing, Johor were selected for this research. The extraction of these samples were carried out using three different polarities of solvents (hexane, ethyl acetate, and methanol). Phytochemical screening were done with various test for each of crude extracts. The results showed that C. aurantifolia gave positive result for alkaloids, saponins, reducing sugar and carbohydrates in hexane and ethyl acetate extracts. In contrast to C. hystrix where the presence of alkaloids, steroids, reducing sugar and carbohydrates were proven in all crude extracts. Lastly, C. microcarpa showed positive result for alkaloids, triterpenoids, saponins, flavonoids, reducing sugar, carbohydrates, phenolic content, glycosides and tannins for hexane and ethyl acetate crude extracts. Through antioxidant assays measured at wavelength 234 nm, C. aurantifoliaa has the highest AOA which was 10. The scavenging effect on DPPH radical was done at the wavelength of 517 nm and C. microcarpa has the highest percentage of scavenging effect with 96.41%. Next, the non-enzymatic antioxidant assays on the determination of α-tocopherol and carotenoids showed that C. hystrix has the highest concentration of α-tocopherol (2.30 ± 0.026), while C. microcarpa has the highest concentration of carotenoids (18.40 ± 2.83).

Subject Areas

Citrus aurantifolia; Citrus hystrix; Citrus microcarpa; phytochemical; antioxidant properties

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