Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Micro RNA Transcriptome Profile in Canine Oral Melanoma

Md. Mahfuzur Rahman
ORCID logo ,
Yu-Chang Lai
,
Al Asmaul Husna
,
Hui-wen Chen
,
Yuiko Tanaka
,
Hiroaki Kawaguchi
,
Noriaki Miyoshi
,
Takayuki Nakagawa
,
Ryuji Fukushima
,
Naoki Miura
* ORCID logo
Version 1 : Received: 26 August 2019 / Approved: 27 August 2019 / Online: 27 August 2019 (16:16:55 CEST)

A peer-reviewed article of this Preprint also exists.

Rahman, M.M.; Lai, Y.-C.; Husna, A.A.; Chen, H.-W.; Tanaka, Y.; Kawaguchi, H.; Miyoshi, N.; Nakagawa, T.; Fukushima, R.; Miura, N. Micro RNA Transcriptome Profile in Canine Oral Melanoma. Int. J. Mol. Sci. 2019, 20, 4832. Rahman, M.M.; Lai, Y.-C.; Husna, A.A.; Chen, H.-W.; Tanaka, Y.; Kawaguchi, H.; Miyoshi, N.; Nakagawa, T.; Fukushima, R.; Miura, N. Micro RNA Transcriptome Profile in Canine Oral Melanoma. Int. J. Mol. Sci. 2019, 20, 4832.

Abstract

MicroRNAs (miRNAs) dysregulation contribute the cancer pathogenesis. However, the miRNA profile of canine oral melanoma (COM), one of the frequent malignant melanoma in dog is still unrevealed. The aim of this study is to reveal the miRNA profile in canine oral melanoma. MicroRNAs profile of oral tissues from normal healthy dogs and COM patients were compared by next-generation sequencing. Along with tumour suppressor microRNAs (miRNAs), we report 30 oncogenic miRNAs in COM. Expression of miRNAs were further confirmed by quantitative real-time PCR (qPCR). Pathway analysis showed that deregulated miRNAs impact on cancer and signalling pathways. Three oncogenic miRNAs targets (miR-450b, 301a, and 223) from human study also were down-regulated in COM and had significant negative co-relation with their respective miRNA. Furthermore, we found that miR-450b expression is higher in metastatic cells and regulated MMP9 expression through a PAX9-BMP4-MMP9 axis. In silico analysis indicated that miR-126, miR-20b, and miR-106a regulated the highest numbers of differentially expressed transcription factors in respect to human melanoma. Chromosomal enrichment analysis revealed the X chromosome was enriched with oncogenic miRNAs. We comprehensively analyzed the miRNA’s profile in COM which will be a useful resource for developing therapeutic interventions in both species.

Keywords

MicroRNAs; next generation sequencing; dog; melanoma

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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