Preprint Article Version 1 This version is not peer-reviewed

Inhibition of Pancreatic α-Amylase Activity by a Weight-Loss Herbal Formula RCM-107

Version 1 : Received: 24 July 2019 / Approved: 25 July 2019 / Online: 25 July 2019 (08:12:19 CEST)

How to cite: Luo, S.; Lenon, G.; Gill, H.; Hung, A.; Dias, D.A.; Li, M.; Nguyen, T.L. Inhibition of Pancreatic α-Amylase Activity by a Weight-Loss Herbal Formula RCM-107. Preprints 2019, 2019070284 (doi: 10.20944/preprints201907.0284.v1). Luo, S.; Lenon, G.; Gill, H.; Hung, A.; Dias, D.A.; Li, M.; Nguyen, T.L. Inhibition of Pancreatic α-Amylase Activity by a Weight-Loss Herbal Formula RCM-107. Preprints 2019, 2019070284 (doi: 10.20944/preprints201907.0284.v1).

Abstract

Reducing carbohydrates digestion by having low glycaemic index (GI) foods has been linked to weight loss. Inhibiting related enzymes is an alternative way to decrease carbohydrate digestion. RCM-107, an eight-herb formula that is modified from the RCM-104, has indicated significant weight-loss action in clinical trials. However, no research has been conducted to study its effect on the activity of porcine pancreatic alpha-amylase (PPA), which is involved in carbohydrate absorption. In this paper, we used fluorescence PPA inhibition assay to investigate the inhibitory effects of RCM-107 and the individual herbs present in this herbal mixture on amylase activity. Subsequently, molecular docking predicted the key active compounds that may be responsible for the enzyme inhibition. According to our results, both the RCM-107 formula and several individual herbs displayed α-amylase inhibitory effects. Also, marginal synergistic effects of RCM-107 were also detected. In addition, alisol B, (-)-epigallocatechin-3-gallate (EGCG) and plantagoside have been predicted as the key active compounds that may be responsible for the α-amylase inhibition effect of RCM-107 according to inter-residue contact analysis. Finally, Glu233, Gln63, His305, Asp300 and Tyr151 are predicted to be markers of important areas with which potential amylase inhibitors would interact.

Subject Areas

obesity; herbal medicine; molecular docking; α-amylase

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