Hong, Y.-H.; Meng, J.; He, X.-L.; Zhang, Y.-Y.; Luan, Y.-S. Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight. Cells2019, 8, 822.
Hong, Y.-H.; Meng, J.; He, X.-L.; Zhang, Y.-Y.; Luan, Y.-S. Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight. Cells 2019, 8, 822.
Hong, Y.-H.; Meng, J.; He, X.-L.; Zhang, Y.-Y.; Luan, Y.-S. Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight. Cells2019, 8, 822.
Hong, Y.-H.; Meng, J.; He, X.-L.; Zhang, Y.-Y.; Luan, Y.-S. Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight. Cells 2019, 8, 822.
Abstract
Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in plant immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Tomato in transgenic plants transiently overexpressed miR482c displayed larger lesion area than the control plants upon infection. Furthermore, compared with the WT tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed decreased expression of target genes accompanied by lower POD, SOD, and PAL activity activities and higher MDA content, thereby leading to a decline in the ROS scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating NBS-LRR expression levels and ROS levels.
Keywords
tomato; late blight; miRNA; overexpression; resistance
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
