Version 1
: Received: 12 March 2019 / Approved: 12 March 2019 / Online: 12 March 2019 (10:28:47 CET)
How to cite:
Ahn, M.Y.; Kim, B.J.; Kim, H.J.; Jin, J.M.; Yoon, H.J.; Hwang, J.S.; Lee, B.M. Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action. Preprints2019, 2019030136. https://doi.org/10.20944/preprints201903.0136.v1
Ahn, M.Y.; Kim, B.J.; Kim, H.J.; Jin, J.M.; Yoon, H.J.; Hwang, J.S.; Lee, B.M. Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action. Preprints 2019, 2019030136. https://doi.org/10.20944/preprints201903.0136.v1
Ahn, M.Y.; Kim, B.J.; Kim, H.J.; Jin, J.M.; Yoon, H.J.; Hwang, J.S.; Lee, B.M. Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action. Preprints2019, 2019030136. https://doi.org/10.20944/preprints201903.0136.v1
APA Style
Ahn, M.Y., Kim, B.J., Kim, H.J., Jin, J.M., Yoon, H.J., Hwang, J.S., & Lee, B.M. (2019). Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action. Preprints. https://doi.org/10.20944/preprints201903.0136.v1
Chicago/Turabian Style
Ahn, M.Y., Jae Sam Hwang and Byung Moo Lee. 2019 "Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action" Preprints. https://doi.org/10.20944/preprints201903.0136.v1
Abstract
Field cricket (Gryllus bimaculatus) is newly emerged as an edible insect in several countries. Anti-inflammatory effect of glycosaminoglycan derived this cricket was not fully investigated on chronic disease animal model such as diabetic mouse. For potential therapeutic agents, anti-diabetic activities of field cricket glycosaminoglycan (GbG) was evaluated in diabetic mice based on their abilities to reduce glucose, ALT, AST, LDL-cholesterol, and BUN levels, compared with dung beetle (Catharsius molossus) glycosaminoglycan (CaG) as a positive control glycosaminoglycan. Db mice were orally administered for one month according to their groups: Db Hetero (normal), Db Homo (type-2 diabetic), CaG (5 mg/kg), GbG (5 mg/kg), and metformin (10 mg/kg). Blood glucose level was decreased after 1st week treatment with GbG. It also inhibited LDL-cholesterol and alkaline phosphatase levels. Regarding oxidative damage of diabetic state, levels of hepatocellular biomarkers levels and protein carbonyl content were reduced in db mice treated with GbG. Especially anti-oxidative activities of catalase, superoxide dismutase, and glutathione peroxidase were significantly increased in GbG treated group compared to those in the control. GbG was composed of heparin disaccharides and main N-glycan was identified as Hex9GlcNAc2 (m/z 1905.7) of with neutral mono-sugar mainly comprising of hexose, L (+) rhamnose by mass spectroscopy. These results from sero-biochemical, hepatocellular anti-oxidant assay in db mice data suggest cricket (G. bimaculatus) glycosaminoglycan might play a role in its anti-diabetic action.
Keywords
cricket glycosaminoglycan; N-glycan; homo db mice; anti-oxidant enzyme
Subject
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
