Preprint Case Report Version 1 This version is not peer-reviewed

Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action

Version 1 : Received: 12 March 2019 / Approved: 12 March 2019 / Online: 12 March 2019 (10:28:47 CET)

How to cite: Ahn, M.Y.; Kim, B.J.; Kim, H.J.; Jin, J.M.; Yoon, H.J.; Hwang, J.S.; Lee, B.M. Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action. Preprints 2019, 2019030136 (doi: 10.20944/preprints201903.0136.v1). Ahn, M.Y.; Kim, B.J.; Kim, H.J.; Jin, J.M.; Yoon, H.J.; Hwang, J.S.; Lee, B.M. Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action. Preprints 2019, 2019030136 (doi: 10.20944/preprints201903.0136.v1).

Abstract

Field cricket (Gryllus bimaculatus) is newly emerged as an edible insect in several countries. Anti-inflammatory effect of glycosaminoglycan derived this cricket was not fully investigated on chronic disease animal model such as diabetic mouse. For potential therapeutic agents, anti-diabetic activities of field cricket glycosaminoglycan (GbG) was evaluated in diabetic mice based on their abilities to reduce glucose, ALT, AST, LDL-cholesterol, and BUN levels, compared with  dung beetle (Catharsius molossus) glycosaminoglycan (CaG) as a positive control glycosaminoglycan.  Db mice were orally administered for one month according to their groups: Db Hetero (normal), Db Homo (type-2 diabetic), CaG (5 mg/kg), GbG (5 mg/kg), and metformin (10 mg/kg). Blood glucose level was decreased after 1st week treatment with GbG. It also inhibited LDL-cholesterol and alkaline phosphatase levels. Regarding oxidative damage of diabetic state, levels of hepatocellular biomarkers levels and protein carbonyl content were reduced in db mice treated with GbG. Especially anti-oxidative activities of catalase, superoxide dismutase, and glutathione peroxidase were significantly increased in GbG treated group compared to those in the control. GbG was composed of heparin disaccharides and main N-glycan was identified as Hex9GlcNAc2 (m/z 1905.7) of with neutral mono-sugar mainly comprising of hexose, L (+) rhamnose by mass spectroscopy. These results from sero-biochemical, hepatocellular anti-oxidant assay in db mice data suggest cricket (G. bimaculatus) glycosaminoglycan might play a role in its anti-diabetic action.

Subject Areas

cricket glycosaminoglycan; N-glycan; homo db mice; anti-oxidant enzyme

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