Preprint Article Version 1 This version is not peer-reviewed

Outflow Facility and Extent of Angle Closure in a Porcine Model

Version 1 : Received: 26 November 2018 / Approved: 27 November 2018 / Online: 27 November 2018 (08:42:09 CET)

How to cite: Hong, Y.; Wang, C.; Loewen, R.; Waxman, S.; Shah, P.; Esfandiari, H.; Loewen, N.A. Outflow Facility and Extent of Angle Closure in a Porcine Model. Preprints 2018, 2018110600 (doi: 10.20944/preprints201811.0600.v1). Hong, Y.; Wang, C.; Loewen, R.; Waxman, S.; Shah, P.; Esfandiari, H.; Loewen, N.A. Outflow Facility and Extent of Angle Closure in a Porcine Model. Preprints 2018, 2018110600 (doi: 10.20944/preprints201811.0600.v1).

Abstract

Purpose: To investigate the extent of anterior chamber angle circumference needed to maintain a physiological outflow facility (C). Methods: Twenty anterior segments of porcine eyes were assigned to 5 groups, each with a different degree of cyanoacrylate-mediated angle closure: 90° (n = 4), 180° (n = 4), 270° (n = 4), 360° (n = 4) and four unoccluded control eyes. The outflow facility was measured at baseline, 3, 12, 24, and 36 hours after angle closure. Outflow patterns were evaluated with canalograms and the histomorphology was compared. Results: Baseline outflow facilities of the five groups were similar (F = 0.922, P = 0.477). Complete, circumferential occlusion over 360° induced a significant decrease in facility from baseline at all time-points (P ≤ 0.023 at 3, 12, 24 and 36 hours). However, no difference from baseline was found in any of the partially-occluded (0–270°) groups (F ≥ 0.067, P ≥ 0.296 at 3, 12, 24 and 36 hours). The canalograms confirmed the extent of occlusion with flow through the unblocked regions. Histology revealed no adverse effects of blockage on the TM or aqueous plexus in the unoccluded angle portions. The unoccluded TM appeared normal. Conclusion: Cyanoacrylate-mediated angle occlusion created a reproducible angle closure model. 90° of unoccluded anterior chamber angle circumference was sufficient to maintain physiological outflow. This model may help understand how outflow can be regulated in healthy, nonglaucomatous TM.

Subject Areas

angle closure model; glaucoma; trabecular meshwork; outflow facility

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