Preprint Article Version 1 This version is not peer-reviewed

Antibacterial Activity of Papain Hydrolysates of Isoelectrically-Isolated Casein and Thermoprecipitated Alpha-Lactalbumin from Bovine and Caprine Milk on Diarrheagenic Bacteria

Version 1 : Received: 13 October 2018 / Approved: 16 October 2018 / Online: 16 October 2018 (11:26:59 CEST)

A peer-reviewed article of this Preprint also exists.

T. Omara (2019). Antibacterial Activity of Papain Hydrolysates of Isoelectrically-isolated Casein and Thermoprecipitated Alpha-Lactalbumin from Bovine and Caprine Milk on Diarrheagenic Bacteria, J. of Advancement in Medical and Life Sciences. V7I3.01. DOI: 10.5281/zenodo.2580653. T. Omara (2019). Antibacterial Activity of Papain Hydrolysates of Isoelectrically-isolated Casein and Thermoprecipitated Alpha-Lactalbumin from Bovine and Caprine Milk on Diarrheagenic Bacteria, J. of Advancement in Medical and Life Sciences. V7I3.01. DOI: 10.5281/zenodo.2580653.

Journal reference: JOURNAL OF ADVANCEMENT IN MEDICAL AND LIFE SCIENCES 2019, 7, 1-6
DOI: 10.5281/zenodo.2580653

Abstract

The study compared antibacterial potential of hydrolysates of casein and alpha-lactalbumin from cow and goat milk on diarrhea-causing Escherichia coli and Staphylococcus aureus. Milk samples were aseptically obtained from lactating cows and goats. The samples were skimmed; casein was isolated using acetic acid and alpha-lactalbumin by filtrate thermoprecipitation at 75 °C. 50% of each isolate was reconstituted in a buffer and hydrolyzed with papain at 55 °C for 2 hours. The hydrolysates were heated to 75 °C to inactivate papain, cooled and their antibacterial activity determined by disc diffusion method. Results showed alpha-lactalbumins had higher degrees of hydrolysis and antibacterial activity than caseins; goat alpha-lactalbumin had the highest antibacterial activity with mean inhibition zones of 19.60 mm and 19.50 mm on E. coli and S. aureus. Cow alpha-lactalbumin inhibited E. coli more than S. aureus with inhibition zones of 16.80 mm and 12.50 mm. Cow and goat milk casein hydrolysates inhibited E. coli with mean inhibition zones of 8.00 mm and 10.90 mm and inhibited S. aureus with inhibition zones of 4.13 mm and 1.90 mm respectively. The research showed that the milk hydrolysates can be a source of antibiotics for diarrhea treatment. Research should be done to establish the peptide fractions associated with the observed bioactivity.

Subject Areas

assay; diarrhea; isolate; hydrolysis; proteins; inhibition zone

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