Preprint Article Version 1 This version is not peer-reviewed

Development of a Novel Electrochemical Inhibition Sensor Array Based on Bacteria Immobilized on Modified Screen-Printed Gold Electrodes for Water Pollution Detection

Version 1 : Received: 18 July 2018 / Approved: 19 July 2018 / Online: 19 July 2018 (11:51:41 CEST)

How to cite: Abu-Ali, H.; Nabok, A.; Smith, T.J.; Al-Shanawa, M. Development of a Novel Electrochemical Inhibition Sensor Array Based on Bacteria Immobilized on Modified Screen-Printed Gold Electrodes for Water Pollution Detection. Preprints 2018, 2018070351 (doi: 10.20944/preprints201807.0351.v1). Abu-Ali, H.; Nabok, A.; Smith, T.J.; Al-Shanawa, M. Development of a Novel Electrochemical Inhibition Sensor Array Based on Bacteria Immobilized on Modified Screen-Printed Gold Electrodes for Water Pollution Detection. Preprints 2018, 2018070351 (doi: 10.20944/preprints201807.0351.v1).

Abstract

The development of a novel and simple inhibition biosensor array for detection of water pollutants based on immobilized bacteria is the main goal of this work. A series of electrochemical measurements (i.e. cyclic voltammograms) were carried out on screen-printed gold electrodes with three types of bacteria, namely Escherichia coli, Shewanella oneidensis, and Methylococcus capsulatus, immobilized via poly L-lysine. For comparison purposes, similar measurements were carried out on bacteria samples in solutions,; also optical measurements (fluorescence microscopy, optical density, and flow cytometry) were performed on the same bacteria in both liquid and  immobilized forms. The study of the effect of heavy metal ions (lead), pesticides (atrazine) and petrochemicals (hexane) on DC electrochemical characteristics of immobilized bacteria revealed a possibility of pattern recognition of the above inhibition agents in aquatic environment.

Subject Areas

electrochemical sensor; inhibition bacteria sensor array; immobilization of bacteria; water pollution; pattern recognition

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