Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biocompatibility of 3D Printed Methacrylate for Hearing Aids and Inner Ear Devices

Version 1 : Received: 17 July 2018 / Approved: 17 July 2018 / Online: 17 July 2018 (16:32:13 CEST)
Version 2 : Received: 23 July 2018 / Approved: 24 July 2018 / Online: 24 July 2018 (12:09:52 CEST)

A peer-reviewed article of this Preprint also exists.

Alifui-Segbaya, F.; George, R. Biocompatibility of 3D-Printed Methacrylate for Hearing Devices. Inventions 2018, 3, 52. Alifui-Segbaya, F.; George, R. Biocompatibility of 3D-Printed Methacrylate for Hearing Devices. Inventions 2018, 3, 52.


The capacity of 3D printing (3DP) technologies to initiate speedy polymerization of solvent free resins accounts for their utility in the manufacturing of medical devices. Nonetheless, independent biological evaluation of 3D printed materials is recommended due to the unique parameters of the manufacturing process, which can influence their physical, chemical, and biological properties. In this study, E-Shell 450 material indicated for 3DP of hearing aid shells and inner ear devices was examined for biological safety using zebrafish bioassays adapted to OECD fish embryo test. In addition, the proprietary material was characterized for composition using headspace gas-chromatography mass spectrometry (GC-MS). To initiate test, newly fertilized zebrafish eggs were cultured on non-treated and ethanol-treated materials in glass petri dishes with ultrapure water, incubated at 28.5°C and assessed for developmental endpoints of toxicity at 24h interval until 96h. Data confirmed non-treated material was extremely toxic in bioassays within 24h whereas ethanol-treated material showed a relative lower toxicity possibly due to ethanoic-aqueous interactions as observed by GC-MS. With the current influx of 3D printing materials, users are urged to exercise caution. Operators must also take cognizance of the potential toxicity of the chemicals used in 3DP and implement safety measures to limit their exposure.


3D printing; biocompatibility; hearing aids; methacrylates; zebrafish embryo model.


Chemistry and Materials Science, Polymers and Plastics

Comments (1)

Comment 1
Received: 19 July 2018
Commenter: Gideon Samevi
The commenter has declared there is no conflict of interests.
  • a very terrific piece of work that will enhance the well being of humanity. text
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