Preprint Article Version 2 This version is not peer-reviewed

Separation of Negatively Charged TiO2-Coated Polystyrene Beads in Microfluidic Device

Version 1 : Received: 13 July 2018 / Approved: 13 July 2018 / Online: 13 July 2018 (11:06:05 CEST)
Version 2 : Received: 17 September 2018 / Approved: 18 September 2018 / Online: 18 September 2018 (10:34:00 CEST)

How to cite: Phiphatanaphiphop, C.; Leksakul, K.; Phatthanakun, R.; Busayaporn, W.; Saiyasombat, C.; Phothongkam, P.; Anshori, I.; Rana, M.; Suzuki, H. Separation of Negatively Charged TiO2-Coated Polystyrene Beads in Microfluidic Device. Preprints 2018, 2018070232 (doi: 10.20944/preprints201807.0232.v2). Phiphatanaphiphop, C.; Leksakul, K.; Phatthanakun, R.; Busayaporn, W.; Saiyasombat, C.; Phothongkam, P.; Anshori, I.; Rana, M.; Suzuki, H. Separation of Negatively Charged TiO2-Coated Polystyrene Beads in Microfluidic Device. Preprints 2018, 2018070232 (doi: 10.20944/preprints201807.0232.v2).

Abstract

This research was presented the special designed microfluidic device generated for sperm separation based on assumption of different surface electrical charged of sperms X and Y. However, to avoid ethical problem, the microfluidic chip has been tested with the mimic electrical charged particles, TiO2-coated Polystyrene beads, (TiO2-coated Ps-beads), instead of spermatozoa. The work has been separated into three main parts. Firstly, the simply but efficient fabrication of negatively charged TiO2-coated Ps-beads has been presented. In addition, various characterization techniques such as X-ray diffraction (XRD), Tungsten Scanning Electron Microscopy (W-SEM) with energy-dispersive X-ray spectroscopy (EDS) mode, and X-ray Absorption Spectroscopy (XAS), have been reported in this work to elucidate the reasons behind the persistence of negatively charged on the surface of TiO2-coated Ps-beads. Results show that  the fabricated TiO2-coated Ps-beads was partly coated in the mixed forms of amorphous Ti4+ and had caused a negatively charge to appear on the surface after fabrication and had sustained its electrical charged for long. Secondly, process of simulation and fabrication of microfluidic device was presented. Finally the negatively charged TiO2-coated Ps-beads were tested in this microfluidic devices. For design of microfluidic devices integrated with a couple of microelectrodes, the simulated structures were fabricated by photolithographic technique and tested with the Ps-beads. Percentage of validation for Ps-beads separation indicated that the 100 mm-distance-between-electrodes microfluidic device exhibits to be the highest performance prototype at 86.96%. For further confirmation, another model so called the single path prototype has been established. It is confirmed by 92.59% of validation for the utilization of the device. The successfully designed microfluidic devices can be examined with actual spermatozoa later. Furthermore, process to fabricate the negatively charged TiO2-coated Ps-beads can be established as testified samples for development of other microfluidic devices.

Subject Areas

microfluidic; microelectrodes; negatively charged; TiO2; assisted reproductive technology

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