Preprint Article Version 1 This version is not peer-reviewed

Engineering Cell Adhesion and Orientation via Ultrafast Laser Fabricated Microstructured Substrates

Version 1 : Received: 24 June 2018 / Approved: 25 June 2018 / Online: 25 June 2018 (10:48:37 CEST)

A peer-reviewed article of this Preprint also exists.

Babaliari, E.; Kavatzikidou, P.; Angelaki, D.; Chaniotaki, L.; Manousaki, A.; Siakouli-Galanopoulou, A.; Ranella, A.; Stratakis, E. Engineering Cell Adhesion and Orientation via Ultrafast Laser Fabricated Microstructured Substrates. Int. J. Mol. Sci. 2018, 19, 2053. Babaliari, E.; Kavatzikidou, P.; Angelaki, D.; Chaniotaki, L.; Manousaki, A.; Siakouli-Galanopoulou, A.; Ranella, A.; Stratakis, E. Engineering Cell Adhesion and Orientation via Ultrafast Laser Fabricated Microstructured Substrates. Int. J. Mol. Sci. 2018, 19, 2053.

Journal reference: Int. J. Mol. Sci. 2018, 19, 2053
DOI: 10.3390/ijms19072053

Abstract

Cells take decisions on their responses depending on the stimuli received by the surrounding extracellular environment, that provides the essential cues at the micro and the nano-lengthscales required for adhesion, orientation, proliferation and differentiation. In this study, discontinuous microcones on silicon (Si) and continuous microgrooves on polyethylene terephthalate (PET) substrates were fabricated via ultrashort-pulsed laser irradiation at various fluences, resulting in microstructures with different roughness and geometrical characteristics. The topographical models attained were specifically developed to imitate the guidance and alignment of Schwann cells for oriented axonal regrowth, towards nerve regeneration. At the same time, positive replicas of the silicon microstructures formed were successfully reproduced, via soft lithography, on the biodegradable polymer poly(lactide-co-glycolide) (PLGA). The anisotropic continuous (PET) and discontinuous (PLGA replicas) microstructured polymeric substrates were assessed in terms of their influence on the Schwann cells responses. It is shown that the developed micropatterned substrates enable control over the cellular adhesion, proliferation and orientation and are thus useful to engineer cell alignment in vitro. This property could be potentially useful in the fields of neural tissue engineering and for dynamic microenvironment systems that simulate in vivo conditions.

Subject Areas

cell adhesion; cell orientation; Schwann cells; topography; laser fabrication; soft lithography; polymeric materials

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.