Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antiproliferative Activity of Neem Leaf Extracts Obtained by a Sequential Pressurized Liquid Extraction

Klebson S. Santos
* ,
Andriele M. Barbosa
,
Victor Freitas
,
Ana Veruska C.S. Muniz
ORCID logo ,
Ricardo C. Calhelha
ORCID logo ,
Isabel C.F.R. Ferreira
ORCID logo ,
Elton Franceschi
,
Francine F. Padilha
ORCID logo ,
Maria Beatriz P.P. Oliveira
ORCID logo ,
Cláudio Dariva
Version 1 : Received: 29 May 2018 / Approved: 30 May 2018 / Online: 30 May 2018 (07:45:25 CEST)

How to cite: Santos, K. S.; Barbosa, A. M.; Freitas, V.; Muniz, A. V. C.; Calhelha, R. C.; Ferreira, I. C.; Franceschi, E.; Padilha, F. F.; Oliveira, M. B. P.; Dariva, C. Antiproliferative Activity of Neem Leaf Extracts Obtained by a Sequential Pressurized Liquid Extraction. Preprints 2018, 2018050439. https://doi.org/10.20944/preprints201805.0439.v1 Santos, K. S.; Barbosa, A. M.; Freitas, V.; Muniz, A. V. C.; Calhelha, R. C.; Ferreira, I. C.; Franceschi, E.; Padilha, F. F.; Oliveira, M. B. P.; Dariva, C. Antiproliferative Activity of Neem Leaf Extracts Obtained by a Sequential Pressurized Liquid Extraction. Preprints 2018, 2018050439. https://doi.org/10.20944/preprints201805.0439.v1

Abstract

Azadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25 ºC and 100 bar, using hexane (SH), ethyl acetate (SEA) and then ethanol (SE) as solvents. An extraction using only ethanol (EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested the highest concentration of terpenes for SEA extract in comparison to SH, SE and EE extracts. Therefore, antiproliferative activity showed SEA extracts were the most efficient inhibitors to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2 between all other extracts studied. However, hepatocellular normal cells were more resistant to SH, SEA, SE, and EE compared to malignant cells of breast, lung, hepatocellular, and cervical. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the normal cells.

Keywords

neem leaves; sequential pressurized liquid extraction; antiproliferative activity

Subject

Medicine and Pharmacology, Pharmacology and Toxicology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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