Preprint Article Version 1 This version is not peer-reviewed

Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mouse via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins

Version 1 : Received: 17 May 2018 / Approved: 18 May 2018 / Online: 18 May 2018 (08:51:37 CEST)

A peer-reviewed article of this Preprint also exists.

Park, E.K.; Lee, H.-J.; Lee, H.; Kim, J.-H.; Hwang, J.; Koo, J.I.; Kim, S.-H. The Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mice via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins. Int. J. Mol. Sci. 2018, 19, 1995. Park, E.K.; Lee, H.-J.; Lee, H.; Kim, J.-H.; Hwang, J.; Koo, J.I.; Kim, S.-H. The Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mice via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins. Int. J. Mol. Sci. 2018, 19, 1995.

Journal reference: Int. J. Mol. Sci. 2018, 19, 1995
DOI: 10.3390/ijms19071995

Abstract

Though melatonin is known to improve ultraviolet B (UVB)-induced oxidative damage and inflammatory conditions via blockade of nuclear factor (NF)-κB, interleukin (IL)-6, there is no report on anti-wrinkle effect of melatonin to date. Hence in the present study, anti-wrinkle mechanism of melatonin was elucidated in UVB treated HaCaT keratinocytes and hairless mice. Herein melatonin protected against a radical initiator tert-Butyl hydroperoxide (t-BOOH) induced reactive oxygen species (ROS) production, matrix metalloprotease 1 (MMP-1) and cytotoxicity in HaCaT keratinocytes. Also, melatonin suppressed the expression of sonic hedgehog (SHH) and GLI for hedgehog signaling, p-NF-kB, cyclooxygenase (COX-2), p-ERK for inflammatory responses in UVB treated HaCaT keratinocytes. Furthermore, melatonin protected skin from wrinkle formation, transdermal water loss in hairless mice irradiated by UVB for 8 weeks. Notably, melatonin prevented against epidermal thickness and dermal collagen degradation in UVB irradiated hairless mice by Hematoxylin & Eosin and Masson’s trichrome staining. Taken together, these findings suggest that melatonin reduces wrinkle formation via inhibition of ROS/SHH and inflammatory proteins such as NF-kB/COX-2/ERK/MMP1.

Subject Areas

melatonin; UVB; HaCaT keratinocytes; collagen; ROS; Hedgehog; Cox-2; MMP-1

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