Preprint Article Version 1 This version is not peer-reviewed

Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mouse via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins

Version 1 : Received: 17 May 2018 / Approved: 18 May 2018 / Online: 18 May 2018 (08:51:37 CEST)

How to cite: Park, E.K.; Lee, H.; Lee, H.; Kim, J.; Hwang, J.; Kim, S. Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mouse via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins. Preprints 2018, 2018050260 (doi: 10.20944/preprints201805.0260.v1). Park, E.K.; Lee, H.; Lee, H.; Kim, J.; Hwang, J.; Kim, S. Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mouse via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins. Preprints 2018, 2018050260 (doi: 10.20944/preprints201805.0260.v1).

Abstract

Though melatonin is known to improve ultraviolet B (UVB)-induced oxidative damage and inflammatory conditions via blockade of nuclear factor (NF)-κB, interleukin (IL)-6, there is no report on anti-wrinkle effect of melatonin to date. Hence in the present study, anti-wrinkle mechanism of melatonin was elucidated in UVB treated HaCaT keratinocytes and hairless mice. Herein melatonin protected against a radical initiator tert-Butyl hydroperoxide (t-BOOH) induced reactive oxygen species (ROS) production, matrix metalloprotease 1 (MMP-1) and cytotoxicity in HaCaT keratinocytes. Also, melatonin suppressed the expression of sonic hedgehog (SHH) and GLI for hedgehog signaling, p-NF-kB, cyclooxygenase (COX-2), p-ERK for inflammatory responses in UVB treated HaCaT keratinocytes. Furthermore, melatonin protected skin from wrinkle formation, transdermal water loss in hairless mice irradiated by UVB for 8 weeks. Notably, melatonin prevented against epidermal thickness and dermal collagen degradation in UVB irradiated hairless mice by Hematoxylin & Eosin and Masson’s trichrome staining. Taken together, these findings suggest that melatonin reduces wrinkle formation via inhibition of ROS/SHH and inflammatory proteins such as NF-kB/COX-2/ERK/MMP1.

Subject Areas

melatonin; UVB; HaCaT keratinocytes; collagen; ROS; Hedgehog; Cox-2; MMP-1

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