Assessment of Green Solvents and Extraction Methods for Biopesticide Preparation from Neem Azadirachta indica Leaves against Oriental Fruit Fly Bactrocera dorsalis (Hendel)

Danila S. Paragas; Kathlia Cruz; Elaida R. Fiegalan

doi:10.20944/preprints201805.0179.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 10 May 2018 / Approved: 11 May 2018 / Online: 11 May 2018 (09:04:45 CEST)

This study was conducted to search for green technology that can extract metabolites from neem leaves for use in the development of botanical pesticide against Bactrocera dorsalis (Hendel) (Diptera:Tephritidae). Rice wine, rice wash, vinegar and distilled water were used as solvents and hot infusion, maceration, hot continuous reflux (Soxhlet), and fermentation were the methods employed. The different leaf extracts prepared by green technology were evaluated for their potentials as pesticide against B. dorsalis. Vinegar extract via Soxhlet extraction (V-S) for eight (8) h registered to have the highest mortality but not significantly different from vinegar - fermentation (V-F), rice wash - Soxhlet (RWa-S), vinegar - maceration (V-M), distilled water - fermentation (DW-F), and rice wash - fermentation (RWa-F) extracts. Phythochemicals present in the extracts are affected by the solvent-extraction interaction. Among the sixteen solvent-extraction interactions, the use of rice wash and fermentation is the most economical method in extracting the extracting the active components of neem leaves against B. dorsalis. Rice wash is a waste that can be utilized in the development of a biopesticide from neem leaves for pest management of B. dorsalis. This is the first report that rice wash is used as extracting solvent in green synthesis.

rice wash; vinegar; rice wine; Soxhlet; fermentation

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Assessment of Green Solvents and Extraction Methods for Biopesticide Preparation from Neem Azadirachta indica Leaves against Oriental Fruit Fly Bactrocera dorsalis (Hendel)

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