Preprint Article Version 1 This version is not peer-reviewed

The Diagnostic Value of HIF-2 Alpha to Determine The Development and Efficacy of Treatment for Contrast Induced Nephropathy: An Experimental Study

Version 1 : Received: 5 May 2018 / Approved: 7 May 2018 / Online: 7 May 2018 (08:15:36 CEST)

How to cite: Altintop, I.; Tatli, M.; Karakukcu, C.; Soyer Sarica, Z.; Yay, A.H.; Balcioglu, E.; Ozturk, A. The Diagnostic Value of HIF-2 Alpha to Determine The Development and Efficacy of Treatment for Contrast Induced Nephropathy: An Experimental Study. Preprints 2018, 2018050106 (doi: 10.20944/preprints201805.0106.v1). Altintop, I.; Tatli, M.; Karakukcu, C.; Soyer Sarica, Z.; Yay, A.H.; Balcioglu, E.; Ozturk, A. The Diagnostic Value of HIF-2 Alpha to Determine The Development and Efficacy of Treatment for Contrast Induced Nephropathy: An Experimental Study. Preprints 2018, 2018050106 (doi: 10.20944/preprints201805.0106.v1).

Abstract

Background and objectives: Contrast-induced nephropathy (CIN), is an acute renal damage due to contrast agents. This study is conducted to determine the potential diagnostic value of hypoxia-inducible factor 2-alpha (HIF2-α) and to evaluate the renal protective effects of N-acetyl cysteine (NAC) and sildenafil in a rat CIN model. Material/Methods: This randomized, controlled, interventional animal study was conducted on Wistar rats. Totally, rats (n = 36) were randomly assigned to four groups: control (n = 9), CIN group (n = 9), CIN+NAC group (n = 9), and sildenafil (n = 9). The rat model was used to form iohexol-originated CIN. During the modelling, prophylactic treatment was performed at 24th and 48th hours. After 48 hours of the modelling; blood, urine, tissue samples were obtained for biochemical analyses. HIF-2-α levels were measured in renal tissue, serum and urine samples. Renal sections were performed in order for histopathologic and immunohistochemical evaluations. Results: In the CIN model, HIF-2α levels and other biochemical parameters were significantly increased (p < 0.01). Both sildenafil and NAC, efficiently decreased the renal damage due to contrast agents (p < 0.05). Similarly, after treatment with sildenafil and NAC, HIF-2α levels were significantly decreased (p < 0.05). Conclusions: The current study constructs an experimental base for the use of HIF-2α for clinical prevention and treatment of CIN. Several mechanisms may be postulated for the changes in HIF-2α levels. Besides, the increased HIF-2α levels with CIN and decreased HIF-2α levels after treatment may be used for the treatment and follow-up of patients with CIN.

Subject Areas

contrast-induced nephropathy; hypoxia-inducible factor 2α; N-acetyl-cysteine; sildenafil

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