Version 1
: Received: 26 April 2018 / Approved: 27 April 2018 / Online: 27 April 2018 (08:05:09 CEST)
How to cite:
Ghoneum, M.H.; Gimzewski, J.K.; Ghoneum, A.D.; Katano, H.; Agrawal, A. Modulation of TRPV1 Function in Human CD4+ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576. Preprints2018, 2018040354. https://doi.org/10.20944/preprints201804.0354.v1
Ghoneum, M.H.; Gimzewski, J.K.; Ghoneum, A.D.; Katano, H.; Agrawal, A. Modulation of TRPV1 Function in Human CD4+ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576. Preprints 2018, 2018040354. https://doi.org/10.20944/preprints201804.0354.v1
Ghoneum, M.H.; Gimzewski, J.K.; Ghoneum, A.D.; Katano, H.; Agrawal, A. Modulation of TRPV1 Function in Human CD4+ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576. Preprints2018, 2018040354. https://doi.org/10.20944/preprints201804.0354.v1
APA Style
Ghoneum, M.H., Gimzewski, J.K., Ghoneum, A.D., Katano, H., & Agrawal, A. (2018). Modulation of TRPV1 Function in Human CD4+ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576. Preprints. https://doi.org/10.20944/preprints201804.0354.v1
Chicago/Turabian Style
Ghoneum, M.H., Hideki Katano and Anshu Agrawal. 2018 "Modulation of TRPV1 Function in Human CD4+ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576" Preprints. https://doi.org/10.20944/preprints201804.0354.v1
Abstract
Transient receptor potential vanilloid (TRPV) channels act as sensors of pain, temperature, and other external stimuli. We have recently shown that DPV576, an aqueous mixture of nanodiamond (ND) and nanoplatinum (NP), can modulate the activity of TRPV on human primary keratinocytes, suggesting their potential as a possible pain modulator [1]. CD4+ T lymphocytes also express TRPV channels, and we sought with special interest to examine the effect of DPV576 in modulating the functions of TRPV channel expression and secretion of cytokines on human CD4+ T lymphocytes. Human primary CD4+ T cells were activated with anti CD3/CD28 with and without DPV576 at 1:25 and 1:100 dilutions for 24 hours in vitro. TRPV Receptor expression (TRPV1 and TRPV4) on CD4+ T cells was examined by flow cytometry. The capacity of DPV576 to modulate the activity of TRPV1 agonist capsaicin in CD4+ T cells was also determined. Activation of CD4+ T cells was determined by production of cytokines TNF-α, IFN-γ, and IL-10 using specific ELISA kits. DPV576 treatment of CD4+ T cells that were activated with anti CD3/CD28, resulted in increased expression of TRPV1 channel but had no effect on TRPV4. This was accompanied by increased secretion of IFN-g and reduced expression of TRPV1 in capsaicin activated CD4+ T cells. In addition, DPV576 inhibited the capsaicin, induced the production of both IFN-g and TNF-α, and enhanced the secretion of IL-10. We conclude that short term exposure to DPV576 inhibits the activity of TRPV1 channels in CD4+ T lymphocytes, which may suggest its possible beneficial use for pain management.
Keywords
DPV576; CD4+ T cells; TRPV1; capsaicin
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
