Preprint Article Version 1 This version is not peer-reviewed

Impact of Pigment Dispersion on Trabecular Meshwork Cells

Version 1 : Received: 30 March 2018 / Approved: 2 April 2018 / Online: 2 April 2018 (07:00:45 CEST)

How to cite: Wang, C.; Dang, Y.; Loewen, R.; Waxman, S.; Shah, P.; Xia, X.; Loewen, N. Impact of Pigment Dispersion on Trabecular Meshwork Cells . Preprints 2018, 2018040007 (doi: 10.20944/preprints201804.0007.v1). Wang, C.; Dang, Y.; Loewen, R.; Waxman, S.; Shah, P.; Xia, X.; Loewen, N. Impact of Pigment Dispersion on Trabecular Meshwork Cells . Preprints 2018, 2018040007 (doi: 10.20944/preprints201804.0007.v1).

Abstract

Purpose: To investigate the effect of pigment dispersion on trabecular meshwork (TM) cells. Methods: Porcine TM cells from ab interno trabeculectomy specimens were exposed to pigment dispersion, then analyzed for changes in morphology, immunostaining, and ultrastructure. Their abilities to phagocytose, migrate, and contract were quantified. An expression microarray, using 23,937 probes, and a pathway analysis were performed. Results: TM cells readily phagocytosed pigment granules. Pigment induced stress fiber formation (pigment (P): 60.1 ± 0.3%, n = 10, control (C): 38.4 ± 2.5%, n = 11, P < 0.001) and contraction at 24 hours onward (P < 0.01). Phagocytosis declined (P: 68.7 ± 1.3%, C: 37.0 ± 1.1%, n = 3, P < 0.001) and migration was reduced after 6 hours (P: 28.0.1 ± 2.3, n = 12, C: 40.6 ± 3.3, n = 13, P < 0.01). Microarray analysis revealed that Rho, IGF-1, and TGFβ signaling cascades were central to these responses. Conclusions: TM cell exposure to pigment dispersion resulted in reduced phagocytosis and migration, as well as increased stress fiber formation and cell contraction. The Rho signaling pathway played a central and early role, suggesting that its inhibitors could be used as a specific intervention in treatment of pigmentary glaucoma.

Subject Areas

pigment dispersion syndrome; pigmentary glaucoma; trabecular meshwork; phagocytosis; migration; contraction; cytoskeleton; gene microarray; Rho signaling pathway

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