Preprint Article Version 1 This version is not peer-reviewed

A Porcine Ex Vivo Model of Pigmentary Glaucoma

Version 1 : Received: 24 March 2018 / Approved: 27 March 2018 / Online: 27 March 2018 (05:22:15 CEST)

How to cite: Dang, Y.; Waxman, S.; Wang, C.; Loewen, R.T.; Sun, M.; Loewen, N.A. A Porcine Ex Vivo Model of Pigmentary Glaucoma. Preprints 2018, 2018030219 (doi: 10.20944/preprints201803.0219.v1). Dang, Y.; Waxman, S.; Wang, C.; Loewen, R.T.; Sun, M.; Loewen, N.A. A Porcine Ex Vivo Model of Pigmentary Glaucoma. Preprints 2018, 2018030219 (doi: 10.20944/preprints201803.0219.v1).

Abstract

Pigment dispersion can lead to pigmentary glaucoma, a poorly understood condition of younger myopic eyes with fluctuating high intraocular pressure. It has been difficult to investigate its pathogenesis without a model similar to human eyes in size and behavior. Here we present a porcine ex vivo model that recreates several features of pigmentary glaucoma, including intraocular hypertension, accumulation of pigment in the trabecular meshwork, and declining phagocytosis. We found that trabecular meshwork cells regulate outflow, form actin stress fibers, and have a decreased phagocytic activity. Gene expression microarrays and a pathway analysis of TM monolayers as well as ex vivo anterior segment perfusion cultures indicated that RhoA plays a central role in regulating the cytoskeleton, motility, and phagocytosis in the trabecular meshwork, providing new insights and targets to investigate in pigmentary glaucoma.

Subject Areas

pigmentary glaucoma; pigment dispersion; intraocular pressure; trabecular meshwork; cytoskeleton; phagocytosis; gene expression microarray; signal pathway

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