Preprint Article Version 1 This version is not peer-reviewed

Characterization of Novel Native Mycoparasitic Trichoderma Isolates from Mangrove Sediments and its Potential Biocontrol against Fusarium spp.

Version 1 : Received: 29 January 2018 / Approved: 29 January 2018 / Online: 29 January 2018 (08:34:07 CET)

How to cite: Filizola, P.R.B.; Luna, M.A.C.; Souza, A.F.D.; Coelho, I.L.; Larnjeira, N.; Campos-Takaki, G.M. Characterization of Novel Native Mycoparasitic Trichoderma Isolates from Mangrove Sediments and its Potential Biocontrol against Fusarium spp.. Preprints 2018, 2018010270 (doi: 10.20944/preprints201801.0270.v1). Filizola, P.R.B.; Luna, M.A.C.; Souza, A.F.D.; Coelho, I.L.; Larnjeira, N.; Campos-Takaki, G.M. Characterization of Novel Native Mycoparasitic Trichoderma Isolates from Mangrove Sediments and its Potential Biocontrol against Fusarium spp.. Preprints 2018, 2018010270 (doi: 10.20944/preprints201801.0270.v1).

Abstract

Native strains of Trichoderma, isolated from mangrove sediments of PE, Brazil were determining their morphological and molecular characterization, and were investigated to assess of their biocontrol potential over the phytopathogenic Fusarium strains isolated from Caatinga soil, PE, Brazil. The Trichoderma strains were characterized by polyphasic approach, which combined their morphological characteristics, macro- and microculture results, growth evaluation by Tukey test, with significance of 5%. The DNA was extracted and the product was amplified with primers ITS 1 and 2, and sequenced. Trichoderma strains were compatible morphologically with the description of the genus. The molecular identification of Trichoderma, sequences of 500 bp were amplified, deposited in GenBank and used for phylogenetic analysis. The growth rate analysis showed rate of 0.1207 cmh-1 to Trichoderma strains and Fusarium spp. lower growth rate (0.031 cmh-1) was observed. The antibiosis tests showed the best antagonistic level of effectiveness to T. asperellum UCP 0149 against F. solani UCP 1395(82.2%) and F. solani UCP 1075(70.0%), followed of T. asperellum UCP 0319 against F. solani UCP1083 (73.4%), and T. asperellum UCP 0168 against F. solani UCP1098 (71.5%), respectively. The data obtained could serve as the basis for developing several biotechnological processes of safe use.

Subject Areas

filamentous fungi; bioactive substances; antibiosis; phytopathogenic

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