Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biological Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative in Human Dental Pulp Cells

Hirohito Kato
,
Yoichiro Taguchi
* ,
Kazuya Tominaga
,
Masahiro Noguchi
,
Kazutaka Imai
,
Ruan Yaru
,
Saitatsu Takahashi
,
Daisuke Kimura
,
Muneyasu Shida
,
Reiko Taguchi
,
Hiroshi Maeda
,
Akio Tanaka
,
Makoto Umeda
Version 1 : Received: 5 January 2018 / Approved: 7 January 2018 / Online: 7 January 2018 (11:00:15 CET)

How to cite: Kato, H.; Taguchi, Y.; Tominaga, K.; Noguchi, M.; Imai, K.; Yaru, R.; Takahashi, S.; Kimura, D.; Shida, M.; Taguchi, R.; Maeda, H.; Tanaka, A.; Umeda, M. Biological Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative in Human Dental Pulp Cells. Preprints 2018, 2018010037. https://doi.org/10.20944/preprints201801.0037.v1 Kato, H.; Taguchi, Y.; Tominaga, K.; Noguchi, M.; Imai, K.; Yaru, R.; Takahashi, S.; Kimura, D.; Shida, M.; Taguchi, R.; Maeda, H.; Tanaka, A.; Umeda, M. Biological Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative in Human Dental Pulp Cells. Preprints 2018, 2018010037. https://doi.org/10.20944/preprints201801.0037.v1

Abstract

Enamel matrix derivative (EMD) is used for periodontal tissue regeneration therapy, and can induce mineralization in dental pulp cells (DPCs). We designed a synthetic peptide (SP) derived from the response of cells to EMD, and investigated the effect of the SP on potentiating osteogenesis in DPCs, which have a critical role of dental pulp homeostasis. DPCs were treated with 0, 10, 100, or 1000 ng/mL SP to determine its effect on cell proliferation, cell migration, cell differentiation, and mineralization. We then examined the molecular effects of the SP, focusing on changes in the mitogen-activated protein kinases (MAPK) signaling pathway in these cells. The SP significantly promoted DPC proliferation and migration. Cultures treated with the SP also showed an enhanced expression of markers of osteogenic differentiation and mineralization. The SP also induced the activation of MAPK signaling pathway components. These results suggest that our SP could promote the dental pulp tissue repair by hard tissue formation and the mineralization through activating MAPK signaling pathway. This study provides the first evidence that SP might be a new material for dental pulp tissue treatment.

Keywords

Emdogain; amelogenin; dental pulp cells; cell differentiation; cell migration; mineralization

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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