Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A New Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures

Matteo Becatti
ORCID logo ,
Rossella Fucci
,
Amanda Mannucci
,
Victoria Barygina
ORCID logo ,
Marco Mugnaini
,
Luciana Criscuoli
,
Claudia Giachini
,
Francesco Bertocci
,
Rita Picone
,
Paolo Evangelisti
,
Francesca Rizzello
,
Cinzia Cozzi
,
Niccolò Taddei
,
Claudia Fiorillo
* ORCID logo ,
Maria Elisabetta Coccia
*
Version 1 : Received: 2 January 2018 / Approved: 3 January 2018 / Online: 3 January 2018 (06:37:39 CET)

A peer-reviewed article of this Preprint also exists.

Becatti, M.; Fucci, R.; Mannucci, A.; Barygina, V.; Mugnaini, M.; Criscuoli, L.; Giachini, C.; Bertocci, F.; Picone, R.; Emmi, G.; Evangelisti, P.; Rizzello, F.; Cozzi, C.; Taddei, N.; Fiorillo, C.; Coccia, M.E. A Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures. Int. J. Mol. Sci. 2018, 19, 592. Becatti, M.; Fucci, R.; Mannucci, A.; Barygina, V.; Mugnaini, M.; Criscuoli, L.; Giachini, C.; Bertocci, F.; Picone, R.; Emmi, G.; Evangelisti, P.; Rizzello, F.; Cozzi, C.; Taddei, N.; Fiorillo, C.; Coccia, M.E. A Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures. Int. J. Mol. Sci. 2018, 19, 592.

Abstract

Oxidative stress negatively affects folliculogenesis and embryo development. However, a reliable and biologically accurate indicator of oxidative stress does not yet exist. On these bases, the aim of this study was to assess -and compare- blood and follicular fluid (FF) redox status in 45 infertile subjects (and 45 age-matched controls) undergoing in vitro fertilization (IVF) and to establish its connection with the outcome of IVF. Blood and FF were obtained at the time of egg retrieval and immediately analyzed. Firstly, ROS production in blood leukocytes and in granulosa cells was assessed. Oxidative stress markers in blood and in granulosa cells resulted significantly (p<0.001) increased in infertile patients compared to controls. Then, a redox index was obtained in plasma and in FF of patients and controls. The main findings emerging from our study in infertile women are: i) blood oxidative stress reflects FF oxidative stress as demonstrated by the significant correlation between blood redox markers and FF redox markers; ii) a significant correlation between oxidative stress parameters and the considered IVF outcomes is present. We suggest the strict monitoring of the redox parameters for the improvement of assisted reproductive techniques success rate and infertility management.

Keywords

oxidative stress; in vitro fertilization (IVF); infertile women; follicular fluid (FF); granulosa cells

Subject

Medicine and Pharmacology, Pathology and Pathobiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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