Preprint Article Version 1 This version not peer reviewed

Inflammatory Bacteriome Featuring Fusobacterium nucleatum and Pseudomonas aeruginosa Identified in Association with Oral Squamous Cell Carcinoma

Version 1 : Received: 5 March 2017 / Approved: 6 March 2017 / Online: 6 March 2017 (04:47:15 CET)

A peer-reviewed article of this Preprint also exists.

Journal reference: Scientific Reports 2017, 7, 1834
DOI: 10.1038/s41598-017-02079-3

Abstract

Studies on the possible association between bacteria and oral squamous cell carcinoma (OSCC) remain inconclusive, largely due to methodological variations/limitations. The objective of this study was to characterize the species composition as well as functional attributes of the bacteriome associated with OSCC. DNA obtained from 20 fresh OSCC biopsies (cases) and 20 deep-epithelium swabs (matched control subjects) were sequenced for the V1-V3 region using Illumina’s 2x300 bp chemistry. High quality, non-chimeric merged reads were classified to species level using a prioritized BLASTN-algorithm. Downstream analyses were performed using QIIME, PICRUSt, and LEfSe. Fusobacterium nucleatum subsp. polymorphum was the most significantly overrepresented species in the tumors followed by Pseudomonas aeruginosa and Campylobacter sp. Oral taxon 44, while Streptococcus mitis, Rothia mucilaginosa and Haemophilus parainfluenzae were the most significantly abundant in the controls. Functionally, genes involved in bacterial mobility, flagellar assembly, bacterial chemotaxis and LPS synthesis were enriched in the tumors while those responsible for DNA repair and combination, purine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, ribosome biogenesis and glycolysis/gluconeogenesis were significantly associated with the controls. This is the first epidemiological evidence for association of F. nucleatum and P. aeruginosa with OSCC. Functionally, an “inflammatory bacteriome” is enriched in OSSC.

Subject Areas

16S rRNA; bacteria; bacteriome; carcinoma; High-Throughput Nucleotide Sequencing; microbiome; mouth; squamous cell

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