Version 1
: Received: 14 February 2017 / Approved: 17 February 2017 / Online: 17 February 2017 (15:02:03 CET)
How to cite:
Spanidis, Y.; Priftis, A.; Stagos, D.; Stravodimos, G.A.; Leonidas, D.D.; Kouretas, D. Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race. Preprints2017, 2017020070. https://doi.org/10.20944/preprints201702.0070.v1
Spanidis, Y.; Priftis, A.; Stagos, D.; Stravodimos, G.A.; Leonidas, D.D.; Kouretas, D. Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race. Preprints 2017, 2017020070. https://doi.org/10.20944/preprints201702.0070.v1
Spanidis, Y.; Priftis, A.; Stagos, D.; Stravodimos, G.A.; Leonidas, D.D.; Kouretas, D. Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race. Preprints2017, 2017020070. https://doi.org/10.20944/preprints201702.0070.v1
APA Style
Spanidis, Y., Priftis, A., Stagos, D., Stravodimos, G.A., Leonidas, D.D., & Kouretas, D. (2017). Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race. Preprints. https://doi.org/10.20944/preprints201702.0070.v1
Chicago/Turabian Style
Spanidis, Y., Demetres D. Leonidas and Demetrios Kouretas. 2017 "Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race" Preprints. https://doi.org/10.20944/preprints201702.0070.v1
Abstract
The aim of this study was to examine the oxidation of human serum albumin (HSA) caused by oxidative stress after an exhaustive exercise such as ultra-marathon race. Thus, blood samples from 12 adult runners who underwent a 103 km mountain ultra-marathon race were collected pre- and 24, 48 and 72 h post race. HSA was partially purified using affinity chromatography and then was subjected to Western blot analysis for disulfide dimers determination, indicating oxidation. The results were correlated with those from a previous study in which the same samples were analyzed using different oxidative stress markers and a good correlation with protein carbonyls (PC) at all time points was observed. Moreover there was a significant correlation with static oxidation-reduction potential (sORP) at 24 h, and a negative correlation with capacity oxidation-reduction potential (cORP) at 24 and 48 h. In addition, an individual analysis of albumin dimers exhibited great inter-individual differences. This inter-individual variability in the oxidation of HSA may suggest different interventions (e.g. through diet) in order to confront the effects on athletes’ organism after a strenuous exercise. In conclusion, this study supported the importance of the assessment of albumin dimers as a predictive marker for exercise-induced oxidative stress.
Keywords
albumin; oxidative stress; exercise; ultra-marathon mountain race; protein oxidation
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
