Yang, Y.; Zhou, L.; Feng, L.; Jiang, J.; Huang, L.; Liu, Q.; Zhang, Y.; Zhang, C.; Liu, Q. Deciphering the Role of Waxy Gene Mutations in Enhancing Rice Grain Quality: From Molecular Insights to Breeding Soft Rice Varieties. Preprints2024, 2024041151. https://doi.org/10.20944/preprints202404.1151.v1
APA Style
Yang, Y., Zhou, L., Feng, L., Jiang, J., Huang, L., Liu, Q., Zhang, Y., Zhang, C., & Liu, Q. (2024). Deciphering the Role of <em>Waxy</em> Gene Mutations in Enhancing Rice Grain Quality: From Molecular Insights to Breeding Soft Rice Varieties. Preprints. https://doi.org/10.20944/preprints202404.1151.v1
Chicago/Turabian Style
Yang, Y., Changquan Zhang and Qiaoquan Liu. 2024 "Deciphering the Role of <em>Waxy</em> Gene Mutations in Enhancing Rice Grain Quality: From Molecular Insights to Breeding Soft Rice Varieties" Preprints. https://doi.org/10.20944/preprints202404.1151.v1
Abstract
Amylose content (AC) stands as a pivotal determinant of rice grain quality, primarily governed by the Waxy gene (Wx), with its allelic diversity significantly influencing AC variation. In the current culinary landscape, soft rice varieties, known for their low AC and superior palatability, are particularly sought after by consumers. Notably, many soft japonica rice varieties cultivated in southern China, including the Nangeng series, are characterized by the presence of the Wxmp allele, derived from the ancestral Wxmq allele. While both alleles are associated with reduced AC, there remains a paucity of detailed understanding regarding the interplay between specific functional single nucleotide polymorphisms (SNPs) within these alleles and the overarching rice grain quality. Employing site-directed mutagenesis, we engineered three distinct transgenic rice lines, each harboring either the Wxmp, Wxmq, or Wxb-5c alleles, against the background of the glutinous rice cultivar Nip(wx). This suite of transgenic rice showcased a gradation ingrain transparency inversely correlated to AC, delineating a sequence from Wxmq, signifying the lowest, through Wxmp, to Wxb-5c, indicating the highest. Consequently, this gradation in AC precipitated variations in other rice grain physicochemical attributes, such as the taste value of cooked rice, gel consistency (GC), and starch pasting properties. Furthermore, analyses of gene expression and enzyme activity revealed that the functional SNPs, Ex4-53G/A and Ex5-53T/C, lead to a decline in the GBSSI enzyme activity without affecting expression levels. These findings underscore the potential of harnessing these SNPs in breeding programs aimed at developing soft rice varieties endowed with enhanced culinary qualities.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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