Lee, K.H.; Lee, N.-E.; Lee, S.-W. In Vitro Self-Circularization Methods Based on Self-Splicing Ribozyme. Int. J. Mol. Sci.2024, 25, 9437.
Lee, K.H.; Lee, N.-E.; Lee, S.-W. In Vitro Self-Circularization Methods Based on Self-Splicing Ribozyme. Int. J. Mol. Sci. 2024, 25, 9437.
Lee, K.H.; Lee, N.-E.; Lee, S.-W. In Vitro Self-Circularization Methods Based on Self-Splicing Ribozyme. Int. J. Mol. Sci.2024, 25, 9437.
Lee, K.H.; Lee, N.-E.; Lee, S.-W. In Vitro Self-Circularization Methods Based on Self-Splicing Ribozyme. Int. J. Mol. Sci. 2024, 25, 9437.
Abstract
In vitro circular RNA (circRNA) preparation methods have been gaining a lot of attention recently as several reports suggest that circRNAs are more stable with better performances in cells and in vivo than linear RNAs in various biomedical applications. Self-splicing ribozymes are considered a major in vitro circRNA generation method for biomedical applications due to their simplicity and efficiency in circularization of gene of interest. This review summarizes updates and discusses recently developed self-circularization methods based on the self-splicing ribozyme such as group I and II intron ribozymes and pros and cons of each method to prepare circRNA in vitro.
Keywords
Circular RNA; Self-circularization; Self-splicing; RNA vaccine; RNA therapeutics; Ribozyme; Group I intron; Group II intron
Subject
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
