Version 1
: Received: 15 May 2024 / Approved: 16 May 2024 / Online: 16 May 2024 (09:55:23 CEST)
How to cite:
Borges, S. D. L.; de Macedo, E. D. S.; da Silva, F. A. V.; Ataíde, B. J. D. A.; Mendes, N. D. S. F.; Passos, A. D. C. F.; de Moraes, S. A. S.; Herculano, A. M.; Oliveira, K. R. H. M.; Bahia, C. P.; Dolabella, S. S.; Batista, E. D. J. O. Experimental Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii. Preprints2024, 2024051067. https://doi.org/10.20944/preprints202405.1067.v1
Borges, S. D. L.; de Macedo, E. D. S.; da Silva, F. A. V.; Ataíde, B. J. D. A.; Mendes, N. D. S. F.; Passos, A. D. C. F.; de Moraes, S. A. S.; Herculano, A. M.; Oliveira, K. R. H. M.; Bahia, C. P.; Dolabella, S. S.; Batista, E. D. J. O. Experimental Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii. Preprints 2024, 2024051067. https://doi.org/10.20944/preprints202405.1067.v1
Borges, S. D. L.; de Macedo, E. D. S.; da Silva, F. A. V.; Ataíde, B. J. D. A.; Mendes, N. D. S. F.; Passos, A. D. C. F.; de Moraes, S. A. S.; Herculano, A. M.; Oliveira, K. R. H. M.; Bahia, C. P.; Dolabella, S. S.; Batista, E. D. J. O. Experimental Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii. Preprints2024, 2024051067. https://doi.org/10.20944/preprints202405.1067.v1
APA Style
Borges, S. D. L., de Macedo, E. D. S., da Silva, F. A. V., Ataíde, B. J. D. A., Mendes, N. D. S. F., Passos, A. D. C. F., de Moraes, S. A. S., Herculano, A. M., Oliveira, K. R. H. M., Bahia, C. P., Dolabella, S. S., & Batista, E. D. J. O. (2024). Experimental Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii. Preprints. https://doi.org/10.20944/preprints202405.1067.v1
Chicago/Turabian Style
Borges, S. D. L., Silvio Santana Dolabella and Evander de Jesus Oliveira Batista. 2024 "Experimental Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii" Preprints. https://doi.org/10.20944/preprints202405.1067.v1
Abstract
Acanthamoeba genus amoebae can affect humans with diseases such as Granulomatous Amebic Encephalitis (GAE), a highly lethal neuroinfection. Several aspects about the disease still need to be elucidated. Animal models of GAE have allowed advances in these aspects. This work tested Wistar rats (Rattus norvegicus albinus) as an animal model of GAE. For this, 32 animals were infected with 1x106 A. castellanii trophozoites, T4 genotype. Ameba recovery tests were carried out on plates of agar, vascular extravasation assays, behavioral tests and histopathological technique with H/E staining. Data were submitted to linear regression analysis, one-way ANOVA and Tukey's test, performed in the GraphPad Prism® 8.0 program, with a significance level of p<0.05. The results revealed the efficiency of the model. There was recovery of amoebae from the liver, lungs and brain of infected animals, significant encephalic vascular extravasation and behavioral changes in these animals, but not in the control animals. However, not all infected animals showed positive histopathology for the analyzed organs. The nervous tissue was the least affected, demonstrating the role of the BBB in the defense of the CNS. Supported by the demonstrated evidence, we confirm the difficulty, but also the feasibility of using rats as an animal model of GAE.
Keywords
Acanthamoeba; Brain; Diagnostic; Experimental infection; Encephalitis; Rat.
Subject
Medicine and Pharmacology, Tropical Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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