Banana Lectin: A Novel Immunomodulatory Strategy for Mitigating Inflammatory Bowel Disease

1. Introduction

Inflammatory bowel diseases (IBDs) are becoming increasingly common around the world and severely affect people's quality of life [1]. These conditions, including Crohn's disease and ulcerative colitis, cause gastrointestinal issues, leading to problems outside the digestive system [2]. People with IBD are more likely to experience various health complications than those without IBD [3]. Additionally, individuals with IBD are less often vaccinated than the general population, which puts them at greater risk for diseases that vaccines can prevent [4]. Given that IBD treatment typically suppresses the immune system, this elevates the risk of infections, thereby amplifying the importance of vaccinations for individuals with IBD [5].

The etiology of IBDs is complex and still needs to be fully understood. These diseases arise from the mucosal immune system's improper reaction to the resident microbiota and other noxious antigens [6]. It was demonstrated that the likelihood of developing IBD is influenced by genetic predispositions and various environmental factors that an individual is exposed to throughout their life, collectively referred to as the exposome [7]. The concept of the exposome accounts for all environmental exposures a person faces, complementing the genome [8]. Among environmental factors, nutrition plays a significant role in influencing the risk and progression of IBD [9], highlighting the importance of considering both our genes and our environment in understanding the disease.

Several studies suggest nutrition is essential to managing IBDs [10,11]. Food provides energy and supplies vital nutrients like amino acids, minerals, and vitamins for our body's optimal metabolism. Additionally, certain food ingredients can directly influence the immune system, acting as immunomodulators, or indirectly, such as prebiotics that support gut health [12]. Given that the state of the immune system, both at the onset and throughout the progression of IBD, significantly affects the severity of the disease, it is understandable why nutrition is a critical element in managing IBD. A well-balanced diet can help mitigate disease severity and improve overall health outcomes for individuals with IBD [13].

Bananas (Mussa sp.) are abundant in everyday nutrition, and many reports on their beneficial impact on the gastrointestinal tract under physiologic and specific pathogenic conditions [14]. Bananas are recommended as a part of the diet in patients suffering from IBDs as they are reported to contribute to the alleviation of disease symptoms [10,15].

Recent findings indicate that a bioactive banana’s ingredient, banana lectin (BanLec), might contribute to the observed beneficial effects. BanLec, a naturally occurring component of bananas, varies in quantity based on the fruit's ripeness and environmental conditions [16]. BanLec, including its naturally occurring and recombinant (rBanLec) isoforms, is stable in the gastrointestinal tract and acts as an immunomodulator [17,18]. (r)BanLec is a T cell mitogen and affects the functional characteristics of T cells and macrophages [19,20,21,22,23].

rBanLec, which closely resembles the natural BanLec in structure and function, can alter the cytokine profile in the colon when applied locally [21]. It induces changes in cytokine production in a dose-dependent manner, helping to regulate the immune response's intensity and supporting a more balanced inflammatory profile [17].

Considering the potential benefits of BanLec, we focused on examining its effects on experimental colitis outcomes when applied orally during the course of the disease. We conducted our experiments on two mouse strains possessing different genetic backgrounds, C57BL/6 (Th1-prone) and BALB/c (Th2-prone) [24], utilizing a model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). We specifically tested the rBanLec isoform's effectiveness as a continuous treatment strategy. The primary objective was to determine rBanLec's potential as an adjunctive treatment for IBD. If our approach proves effective, it can advance the treatment of IBD, positively impact patient well-being, and indirectly increase vaccination uptake rates by mitigating the symptoms associated with IBD.

2. Materials and Methods

2.1. Experimental Design

For our experiments, we used ten-week-old BALB/c and C57BL/6 mice, each weighing approximately 20 grams. rBanLec (GenBank accession number EU055641) used for treatments was produced and purified according to the previously described procedure [18,25] and dissolved in phosphate-buffered saline (pH=7.4; PBS) to make solutions with rBanLec concentrations of 0.1, 1, or 10 µg/ml. Before application, all rBanLec solutions were sterilized by filtration through Ø 0.22 µm filters. Apart from a required overnight fast before receiving intrarectal treatments, animals took food and water ad libitum.

2.1.1. Induction of Experimental Colitis

Experimental colitis was induced by a single-dose intrarectal administration of TNBS dissolved in 50 % ethanol (25 mg/ml, 100µl/dose; 2 mg TNBS/dose) on overnight fasted mice under anesthesia (10 mg/ml ketamine and 1 mg/ml xylazine in PBS, ~100 µl/mice, intraperitoneally). The day of experimental colitis induction was allotted as day 0.

2.1.2. rBanLec Dosage in a Therapeutical Setting

rBanLec treatment upon the induction of experimental colitis involved daily administration, by oral route (100 µl/mice), of solutions containing 0.1, 1, and 10 µg/ml rBanLec. Thus, mice received 0.5, 5, and 50 mg rBanLec/kg BW/day.

2.1.3. Treatment with rBanLec after the Induction of Colitis and Monitoring of Mice

rBanLec therapy was initiated at the onset of the disease and was maintained consistently for a period of one week, from day 0 through day 7. Depending on the rBanLec dosage, mice of both strains were divided into three groups (n=15 mice/group). Three groups assigned: (1) rBL0.1, (2) rBL1, and (3) rBL10 were treated with solutions (100 µl/day) containing 0.1, 1, and 10 µg/ml rBanLec, respectively. Additionally, we included two control groups to scale the effects of rBanLec against both 1) standard disease progression and 2) a non-diseased state in both strains used. The Negative Control (NC) group, comprising 15 age-matched mice, was administered a single intrarectal dose of 100 µl of 50% ethanol on day 0. This served to observe the effects of a vehicle without the active disease inducer. The Positive Control (PC) group, also with 15 age-matched mice, received a 100 µl of 25 mg/ml of TNBS dissolved in 50% ethanol intrarectally on day 0, replicating the disease conditions but without the administration of rBanLec, to establish the baseline disease response.

We monitored the body weight (BW) of the animals daily. Changes in BW, expressed as percentages, were used as a primary indicator of disease severity. From each group, five mice were randomly selected and euthanized at the peak of the disease (day 2 for C57BL/6 mice, day 3 for BALB/c mice), and another ten mice were observed until day 7, the recovery phase. Colon samples collected from the euthanized mice were prepared for tissue homogenates. Segments of colons collected from C57BL/6 mice were processed for histological and flow cytometric analysis. Additionally, we measured and recorded the length of these colons at the peak of the disease.

2.2. Histochemical Analysis

Histological analyses were performed only for colons collected from C57BL/6 mice as they are considered to be more susceptible to organ-specific Th1-mediated immunopathology [26,27,28,29]. Colon tissue specimens were rinsed with PBS and fixed in 4 % formalin. Fixed colon samples were dehydrated in an ethyl alcohol series, cleared in xylene, and embedded in paraffin at 55 ^oC. Tissue sections (thickness 5μm) were cut on a microtome (Leica RM 2155) and mounted on superfrost glass slides (Thermo Scientific). Primary histological analysis was performed on sections subjected to the hematoxylin/eosin (HE) staining according to the standard procedure. HE-stained sections were analyzed using an Olympus BH2-RFL light microscope (Olympus Optica Ltd, Tokyo, Japan) with a High-Resolution Digital Camera (Color View III, Olympus Soft Imaging Solutions, Munster, Germany). An experienced pathologist who was blinded for the experimental groups performed histopathology analysis. For confocal microscopy, tissue sections were stained using an anti-mouse CD45 antibody conjugated with phycoerythrin (Biolegend) and counter-stained with propidium iodide. After rinsing in PBS, the slides were mounted with a Fluorescent Mounting Medium (Dako, Glostrup, Denmark). Images were captured by an inverted confocal microscope Zeiss Laser scanning system LSM 510 Meta (Carl Zeiss, Jena, Germany) and processed with Zeiss LSM Image Browser software.

2.3. Analysis of T Regulatory cells (Treg) by Flow Cytometry

Flow cytometric analysis was performed on single-cell suspensions prepared from mesenteric lymph nodes (MLN) that were isolated from C57BL/6 mice at the peak of the disease (day 2) and during the recovery (day 7). MLNs were mechanically disrupted in RPMI1640 medium supplemented with 5% FCS. The obtained cell suspension was passed through a strainer (pore size 70 µm). Cells were washed in ice-cold 2% BSA/0.01% NaN3/PBS. Staining (30 min at 4 °C in the dark) of surface markers CD4 and CD25 was done by specific monoclonal antibodies conjugated with phycoerythrin and allophycocyanin, respectively (Biolegend), appropriately diluted in 2% BSA/0.01% NaN3/PBS. After washing, cells were subjected to fixation and permeabilization by Foxp3/transcription Factor Staining Buffer Set (eBioscience) according to the manufacturer’s instructions. Foxp3 was stained by a specific monoclonal antibody conjugated with fluorescein isothiocyanate (Biolegend). Cells were analyzed by BD FACSVerse™ flow cytometer (BD Bioscience, USA) equipped by CellQuest Software.

2.4. Preparation of Colon Homogenates

The colon was rinsed with a cold PBS, weighted and embedded in the buffer for homogenization (cold PBS supplemented with 1mM EDTA, 0.1% NP-40, 1% Triton X-100, and 1% cocktail of protease inhibitors (Serva)). Upon homogenization (Janke &Kunkel, Staufen, Germany), samples were centrifuged (18000 x g, 10 min, 4 °C; Centrifuge 3K-18, Sigma, Osterode, Germany), and supernatants were collected for further analyses. The total protein concentration in supernatants was determined following Bredford’s method. Supernatants were stored at -80 °C until further analysis.

2.5. Evaluation of Local NO Production

NO production was evaluated by quantification of its stable end product (nitrite) using a Griess reagent. An equal volume of Griess reagent (1% sulphanilamide, 0.1% N-(1-naphthyl)-ethylenediamine dihydrochloride in 5% phosphoric acid) and fresh supernatants of homogenized colonic samples (100 µ/well) was added to the 96-well plate and incubated for 10 min at room temperature (RT). After incubation, the absorbances were measured at 545 nm, and concentrations were calculated from a linear standard curve prepared using sodium nitrite.

2.6. Evaluation of Local Myeloperoxidase (MPO) Activity

The evaluation of MPO activity was based on MPO's oxidation of o-phenylenediamine (Sigma). Supernatants of colonic homogenates were adjusted to the same protein concentration (1 mg/ml) before analysis. Equal volumes (75 µl) of supernatant and substrate solution (1 mg/ml phenylenediamine, 0.01% hydrogen peroxide, 50 mM citric acid, pH=5) were mixed in a 96-well plate (in a total of 50 µl/well). After incubation for 10 min at RT, the reaction was terminated by adding 50 µl/well of 1M sulphuric acid. The absorbance was measured at 492 nm and 620 nm (A492/620).

2.7. Analysis of IL-10 and TNFα Production

Concentrations of IL-10 and TNFα were measured by a sandwich ELISA in the supernatants of the colon homogenates collected at the peak of TNBS-induced colitis. Capturing antibody against either IL-10 (eBioscience; 2 µg/ml in PBS) or TNFα (Biolegend; 3 µg/ml in PBS), respectively, was adsorbed (50 µl/well; overnight at 4 °C) onto a microtiter plate (MaxiSorp; Nunc, Roskilde, Denmark). Upon blocking with 1% w/v BSA/PBS, supernatants and commercially available standards (1:2 serial dilutions prepared in 1% w/v BSA/PBS) were added to the wells (50 µl/well; 1h at RT). IL-10 and TNFα were detected with biotin-labeled anti-mouse IL-10 (eBioscience; 1 µg/ml in 1% w/v BSA/PBS) and anti-mouse TNFα (Biolegend; 0.5 µg/ml in 1% w/v BSA/PBS), respectively (50 µl/well; 1h at RT). Blocking and each subsequent step was followed by a washing step with a 0.05% v/v Tween 20/ PBS (4 x 200 µl/well). ExtrAvidin-peroxidase (Sigma, USA) / OPD system was used for the visualization, and absorbance was read at 492 nm and 620 nm (Multiscan Ascent, Labsystems).

2.8. Statistical Analysis

The statistical significance of differences in parameter values measured at specified time-point was assessed using a One-way ANOVA, followed by Bonferroni's multiple comparison test. Additionally, Pearson's bivariate correlation analysis was employed to calculate Pearson's correlation coefficient (Pcc), which reflects the relationship between specific variables. A probability (p) value 0.05 was considered the threshold for significance for all analyses.

3. Results

3.1. Oral Application of rBanLec throughout TNBS-Induced Colitis Reduces the Disease Severity in a Dose-Dependent Manner

Induction of colitis in the experimental setup led to marked weight loss and a decrease in colon length at the disease's peak in both BALB/c (Figure 1A) and C57BL/6 (Figure 1B) mouse strains. Notably, the weight loss in the PC group at the peak of the disease was statistically significant when compared to the corresponding NC group, with a p-value less than 0.001 for both strains. Similarly, a significant shortening of colon length was observed in the PC group compared to the NC group, with a p-value less than 0.005 and 0.001 for C57BL/6 and BALB/c mice, respectively (Figure 1C,D). The follow-up in the BW fluctuations in C57BL/6 and BALB/c mice orally treated (daily) with rBanLec upon the induction of colitis showed that the application of rBanLec in doses of 0.1 µg/ml exerts the most pronounced benefits (Figure 1A,B) in both strains.

Considering the weight loss at the peak of the disease, a significant beneficial effect in BALB/c was recorded only in mice receiving rBanLec in a concentration of 0.1 µg/ml (p˂0.005; Figure 1A). However, a reduction in colon length recorded at the peak of the disease implied the beneficial effects in rBL1 and rBL10 groups as well (compared to the PC group: p˂0.005 for rBL0.1 and rBL1, p˂0.05 for rBL10; Figure 1C,E).

In C57BL/6 mice, the beneficial effect at the peak of the disease was recorded in all rBanLec-treated groups (compared to the PC group: p˂0.001 for rBL0.1, p˂0.05 for rBL1 and rBL10; Figure 1B). In addition, a reduction in colon length at the peak of the disease was the lowest in the rBL0.1 group (p˂0.005 compared to PC and rBL10, p˂0.05 compared to for rBL1; Figure 1D,F).

Treatment with rBanLec demonstrated a beneficial effect on the progression of colitis, particularly in BALB/c mice, even when administered in higher doses. This positive influence is reflected in a more robust weight recovery phase. When compared to the PC group, BALB/c mice treated with lower doses of rBanLec (rBL0.1) showed a statistically significant improvement in weight gain, with a p-value less than 0.001, and those treated with a higher dose (rBL1) also showed significant improvement, with a p-value less than 0.05, as depicted in Figure 1A. In C57BL/6 groups, both doses, rBL0.1 and rBL1, resulted in a highly significant weight gain compared to the PC group, with p-values less than 0.001, and the highest dose (rBL10) also showed significant weight gain, with a p-value less than 0.005, as illustrated in Figure 1B.

3.2. Treatment with rBanLec Reduces Infiltration of Immune Cells in the Colon at the Peak of the Disease

Histological examination of the colon tissue from C57BL/6 mice at the peak of the disease, as shown in Figure 2, indicates that the mice treated with rBanLec retained a more intact colonic architecture and exhibited less infiltration by immune cells, identified as CD45+ cells (details provided in Appendix 1) when compared to the mice in the PC group.

Histochemical analysis of colon samples collected at the peak of the disease (Figure 2) revealed that the massiveness of cell infiltrates and the extent of crypts deformation positively correlate to the disease severity assessed by weight loss (Figure 1A,B). Analysis of PC sections collected at the peak of the disease revealed pronounced impairment of the architecture of colonic tissue associated with the intensive destruction of goblet cells and scattering of infiltrates of immune cells along all tissue layers, yet mostly in lamina propria. Attenuation and deformation of the crypts were marked in tissue sections of rBanLec-treated mice, and they were the lowest in the rBL0.1 group. The hypertrophy of lamina muscularis externa as a response to the inflammation was observed in the rBL1 and rBL10 groups. Infiltrations of the immune cells were marked in all sections of rBanLec-treated mice, but in rBL10 mice, they were the most pronounced and associated with hemorrhagic zones in some places.

In addition to histological analysis of the colon, MLN draining colonic mucosa of C57BL/6 mice were analyzed for the presence of Treg (identified as CD4+CD25+Foxp3+; Figure 3A) at the peak of the disease and during the recovery phase. Irrespective of the rBanLec treatment, MLN collected from C57BL/6 mice subjected to the induction of experimental colitis contained a higher percentage of Treg at the peak of the disease compared to NC (p˂0.05). Furthermore, there were no statistically significant differences among percentages recorded for PC and rBanLec-treated groups at that time point. However, during the recovery phase (day 7), the percentage of Treg in the MLN of the rBL0.1 group was significantly higher compared to the MLN of rBL10 (p˂0.05) as well as PC (p˂0.005) group (Figure 3B).

3.3. Treatment with rBanLec Is Associated with Reductions in Local MPO Activity and NO Production at the Peak of the Disease

Measurements of local MPO activity and NO production, which are often elevated in inflammation, implied the dose-dependent anti-inflammatory impact of rBanLec treatment (Figure 4). Indeed, compared to the basal level recorded in the corresponding NC, those parameters were significantly elevated in PC at the peak of the disease in both strains (BALB/c: p˂0.001 for both MPO activity and NO production; C57BL/6: p˂0.005 for MPO activity and p˂0.001 for NO production). Although some strain-dependent differences were marked, a low rBanLec dose (0.01 µg/ml, 100 µl per day) exerted a more substantial beneficial impact than the highest one (10 µg/ml, 100 µl per day). In BALB/c mice, both MPO activity and NO production at the peak of the disease were significantly lower in all rBanLec-treated groups compared to PC (MPO activity: p˂0.005 for rBL0.1 and rBL1, and p˂0.05 for rBL10; NO production: p˂0.001 for rBL0.1 and rBL1, p˂0.005 for rBL10). In C57BL/6 mice, rBanLec treatment significantly reduced MPO activity only in the rBL0.1 group (p˂0.005), while NO production was significantly reduced in all rBanLec-treated groups (p˂0.001 for rBL0.1, rBL1 and rBL10 groups) compared to PC.

Recovery from the disease for all mice subjected to the induction of experimental colitis was associated with a decline in local NO production and MPO activity compared to the peak of the disease. MPO activity and NO production recorded in recovered PC C57BL/6 mice reached levels comparable to the ones recorded in the NC, while in recovered PC of BALB/c mice, they remained higher than basal levels (PC vs. NC p˂0.005 for both parameters). In rBanLec-treated groups of BALB/c mice, MPO activity and NO production were lower than in corresponding PC (MPO activity: p˂0.05 for rBL0.1 and rBL1, NO production: p˂0.05 for rBL0.1, rBL1, and rBL10), reaching the level recorded in BALB/C NC group. The same trend was marked for NO production in recovered C57BL/6 mice (compared to PC p˂0.05 for rBL0.1 and rBL10). In rBL1, rBL10, and PC groups of C57BL/6 mice, local MPO activity at recovery was lower compared to the peak of the disease, reaching a level comparable to NC. Among C57BL/6 mice subjected to the induction of experimental colitis, the highest MPO activity in the recovery was recorded in the rBL0.1 group. Even more, it was higher than at the peak of the disease (p˂0.005).

3.4. Treatment with rBanLec Modulates the Local [TNFα]/[IL-10] Ratio at the Peak of the Disease

The measurement of local concentrations of the TNFα and IL-10 cytokines with a pronounced pro-inflammatory and anti-inflammatory impact, respectively, confirmed that an increase in the contribution of the anti-inflammatory immune response accompanied the reduction in disease severity at the peak of the disease. Irrespective of the rBanLec treatment, induction of experimental colitis in both strains was associated with a simultaneous rise in local production of both TNFα and IL-10 (Figure 5).

Among groups subjected to the induction of experimental colitis, the most intensive increase of local concentrations of TNFα at the peak of the disease was recorded in PC groups for both strains. In BALB/c mice, local concentrations of TNFα and IL-10 at the peak of the disease were significantly higher in PC (p˂0.001 for both cytokines) as well as rBanLec-treated mice (IL-10: p˂0.005 for rBL0.1, p˂0.001 for rBL1 and rBL10; TNFα: p˂0.005 for rBL0.1 and rBL1, p˂0.001 for rBL10) than basal concentrations recorded in NC. A significant increase in local TNFα concentration at the peak of the disease was recorded in C57BL/6 mice as well (compared to NC: p˂0.05 for rBL0.1, p˂0.005 for rBL10, p˂0.001for rBL1 and PC). The increase in local IL-10 concentration at the peak of the disease was not pronounced in either group of C57BL/6 mice subjected to the induction of experimental colitis, although it was significantly higher in rBanLec-treated groups than in PC (p˂0.05 in rBL1).

In addition, analysis of local [TNFα]/[IL-10] at the peak of the disease revealed it’s decreasing due to rBanLec treatment (Figure 6A), most intensively in rBL0.1 groups of both strains (rBL0.1 compared to corresponding PC: p˂0.005 for both strains). Correlation analysis summarizing presented data revealed a significant correlation between the severity of pathology (estimated via body weight loss) and a local [TNFα]/[IL-10] ratio at the peak of disease (Pcc= -0,951, p˂0.05 for BALB/c, Pcc= -0.909, p˂0.05 for C57BL/6; Figure 6B).

The recovery was associated with a sharp drop in local TNFα concentration (Figure 5), which in all groups subjected to the induction of experimental colitis except the PC group of BALB/c mice (PC vs. NC p˂0.005 for BALB/c) reached a level comparable to the one recorded in corresponding NC. Contrary to the groups of C57BL/6 mice subjected to the induction of experimental colitis where local IL-10 levels were similar to NC (Figure 5), local IL-10 levels remained elevated in recovered BALB/c mice (compared to NC: p˂0.005 for rBL0.1, p˂0.001 for rBL1, rBL10 and PC).

4. Discussion

The presented results show that rBanLec significantly modulates the local immune response in the colon by reducing the severity and supporting the recovery from experimental colitis.

Intrarectal TNBS administration to mice induces transmural colitis mainly driven by a Th1-mediated immune response and characterized by local infiltration of the lamina propria with T cells, neutrophils, and macrophages as well as some clinical features of Crohn’s disease (severe diarrhea, weight loss, and rectal prolapse) [30]. Hence, it is widely used in the study of immunologic aspects relevant to Crohn’s disease. Testing of rBanLec therapeutic potential was performed on two mouse strains, BALB/c and C57BL/6, which are highly different concerning susceptibility to inflammatory-mediated diseases, including IBDs [31]. Experimental colitis could be induced in both strains, although there are conflicting data on strain-specific susceptibility toward induction of experimental colitis depending on the applied method. This could be attributed to the specific characteristics of gut microbiota and differences in GALT-related immunological profiles encompassing the proportion of T cell populations in GALT and draining lymph nodes as well as the specific kinetics of expression of various mediators during the establishment of an immune response [24]. Those factors result in generally stronger inflammatory responses to the same stimuli in C57BL/6 compared to BALB/c mice. Hence, BALB/c mice are considered to be more resistant to organ-specific Th1-mediated immunopathology than C57BL/6 mice [26,27,28,29]. Furthermore, as demonstrated for peritoneal macrophages, the response to rBanLec stimulation of C57BL/6 and BALB/c mice is not the same [24].

Although the therapeutic administration of rBanLec in a tested dose range (0.01 – 1 µg/dose) could not fully prevent the development of experimental colitis, important beneficial effects were observed. The efficacy of rBanLec as a treatment for experimental colitis was notably dose-dependent. Post-induction therapeutic application of rBanLec revealed that its advantageous effects were more evident with lower doses. Following colitis induction, a daily regimen of rBanLec at a minimal dose (0.01 µg/dose) led to substantial mitigation of disease severity in both mouse strains. However, even in higher doses, rBanLec positively impacted the overall course of the disease.

The positive effects of rBanLec were evident in the moderated weight loss and the reduced shortening of the colon during the most intense phase of the disease. These observable changes could be attributed to decreased cellular infiltration in the colonic tissue and the initiation of an anti-inflammatory immune response following the onset of colitis. The suppression of inflammation in the colon was further indicated by increased production of the regulatory cytokine IL-10 and a diminished pro-inflammatory response, as evidenced by the lowered ratio of TNFα to IL-10, decreased NO production, and reduced MPO activity.

In our study, we posit that the upregulation of pro-inflammatory cytokines may necessitate a higher level of rBanLec stimulation compared to what is required for augmenting the production of regulatory cytokines. The observed ratio of local IL-10 to TNFα concentrations at the apex of the disease suggests that lower doses of rBanLec tip the balance in favour of an anti-inflammatory environment (evidenced by higher IL-10 levels) rather than an inflammatory one (indicated by lower TNFα levels). The results point to strain-specific pathways for the observed reduction in the local [TNFα]/[IL-10] ratio in response to the rBanLec treatment. Specifically, this was attributed to a decrease in TNFα production in C57BL/6 mice, while a notable increase in IL-10 production was instrumental for the BALB/c mice. A similar observation related to strain-specific kinetic of IL-10 expression in a mouse model of DSS-induced colitis was reported by Mukhopadhyay et al [31]. Further, different MPO kinetics during recovery of C57BL/6 and BALB/c mice imply additional strain-specific differences in mechanisms underlining recovery. It implies the important role of neutrophils in the resolution of inflammation in C57BL/6 mice [32]. Besides the alleviation of local inflammation, it might as well be that range, as a TLR-2 agonist [23], supported the restoration of compromised epithelial integrity [33].

The immune system's attempts to regulate colonic inflammation are reflected in the augmented differentiation of Tregs within the MLNs. At the climax of the disease, the increase in Treg proportions was consistent across all diseased mice, whether treated with rBanLec or not, indicating a systemic attempt to limit inflammation. Notably, the elevated Treg levels were sustained during the recovery phase exclusively in the cohort treated with a low dose of rBanLec (rBL0.1). This could suggest a prolonged and enhanced capacity of the immune system to forestall further intense inflammatory responses.

Our findings reveal that both rBanLec and BanLec-containing foods hold significant promise for treating IBDs. We have demonstrated that rBanLec can positively influence the local environment in the colon, promoting regulatory and anti-inflammatory responses. However, the finding that the impact of rBanLec is highly dose-dependent highlights the importance of careful consideration in the formulation of rBanLec-based treatments. Daily treatment with 0.01 µg rBanLec (i.e., 0.5 mg rBanLec/kg BW/day) was the most efficient IBD attenuation in both mice strains. That dosage extrapolated to humans would be ~0.04 mg rBanLec/kg BW/day [34]. That therapeutic dosage for a human weighing 70 kg (~2.8 mg BanLec) could be found in one banana (average BanLec content is ~4 mg/100 g banana pulp [35]; a medium-sized banana contains ~120 g of banana pulp [36]. As we observe certain differences in different genetic backgrounds, BanLec could be a part of a personalized application strategy in the future.

Elucidating the underlying processes by which rBanLec confers its therapeutic benefits is pivotal for the advancement of a potent and secure treatment regimen for IBD using BanLec.

5. Conclusions

Should our results be confirmed through further research, it could offer a new avenue for the treatment and management of IBD patients and increase the likelihood of these patients vaccinating more frequently. This is particularly significant considering the compromised immune systems of IBD patients and their increased risk for vaccine-preventable diseases. In conclusion, our study highlights the potential of BanLec as a key component in advancing IBD treatment and improving patient outcomes.