Haideri, M.H.; Phanjaroen, T.; Khiaolaongam, W.; Laoung-on, J.; Hongsibsong, S.; Boonyapranai, K.; Ounjaijean, S. Column Chromatographic Fractionated of Gymnema inodorum Leaves Extracted: Antioxidant Activity and Their Phenolic Profiles. Preprints2024, 2024020624. https://doi.org/10.20944/preprints202402.0624.v1
APA Style
Haideri, M.H., Phanjaroen, T., Khiaolaongam, W., Laoung-on, J., Hongsibsong, S., Boonyapranai, K., & Ounjaijean, S. (2024). Column Chromatographic Fractionated of <em>Gymnema inodorum</em> Leaves Extracted: Antioxidant Activity and Their Phenolic Profiles. Preprints. https://doi.org/10.20944/preprints202402.0624.v1
Chicago/Turabian Style
Haideri, M.H., Kongsak Boonyapranai and Sakaewan Ounjaijean. 2024 "Column Chromatographic Fractionated of <em>Gymnema inodorum</em> Leaves Extracted: Antioxidant Activity and Their Phenolic Profiles" Preprints. https://doi.org/10.20944/preprints202402.0624.v1
Abstract
In recent years, growing attention has been directed towards the potential of plant extracts as great therapeutic agents in controlling oxidative stress and inflammation. Gymnema inodorum (Lour.) Decne, an indigenous plant locally grown in Northern Thailand, is known for its anti-diabetic activity. This study aims to explore the antioxidant properties and phenolic profile of column chromatographic fractionated G. inodorum leaf extract. The ethanolic-reflux extracts of G. inodorum leaves were subjected to column chromatography for the isolation of bioactive molecules. Total phenolic and flavonoid content were assessed using Folin–Ciocalteu and aluminium chloride colorimetric methods, respectively. Total antioxidant activity was evaluated by employing different antioxidant assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic)acid (ABTS) scavenging assay and ferric reducing antioxidant power (FRAP) assay. High-Performance Liquid Chromatography (HPLC) with a C18 column was employed to determine the level of gymnemic acid, while Liquid Chromatography Quadrupole Time-of-Flight/Mass Spectrometry (LC-QTOF/MS) was used to identify potential phenolic compounds. The three separated fractions indicating A, B, and C, exhibited remarkable antioxidant activity, with total phenolic content of 0.294 ± 0.056, 0.085 ± 0.039, and 0.227 ± 0.041 mg QE/mL, and total flavonoid content of 0.274 ± 0.050, 0.309 ± 0.110, and 0.384 ± 0.007 mg QE/mL, respectively. Fraction A exhibited the highest DPPH and ABTS scavenging activity, while fraction C demonstrated significantly higher ferric-reducing power. HPLC analysis revealed a highest concentration of gymnemic acid in fraction C (0.393 ± 0.003 mg/g) compared to fraction A (0.159 ± 0.003 mg/g) and fraction B (0.142 ± 0.000 mg/g). The LC-QTOF/MS phenolic screening showed seven potential phenolic compounds in fraction A, seven compounds in fraction B, and four compounds in Fraction C. A significant relationship is found between antioxidant potential and total phenolic content, suggesting that phenolic compounds are the major contributors to the antioxidant activity of G. inodorum.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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