Version 1
: Received: 5 February 2024 / Approved: 5 February 2024 / Online: 5 February 2024 (15:12:44 CET)
How to cite:
Kang, H.L.; Kupai, K.; Valkusz, Z.; Szasz, A.; Várkonyi, T.; Csonka, Á.; Pósa, A.; Matusovits, D. Neutrophil Activation in Diabetes Mellitus (DM)? Role of PAD4 Expression in STZ-Induced Diabetic Rat. Preprints2024, 2024020298. https://doi.org/10.20944/preprints202402.0298.v1
Kang, H.L.; Kupai, K.; Valkusz, Z.; Szasz, A.; Várkonyi, T.; Csonka, Á.; Pósa, A.; Matusovits, D. Neutrophil Activation in Diabetes Mellitus (DM)? Role of PAD4 Expression in STZ-Induced Diabetic Rat. Preprints 2024, 2024020298. https://doi.org/10.20944/preprints202402.0298.v1
Kang, H.L.; Kupai, K.; Valkusz, Z.; Szasz, A.; Várkonyi, T.; Csonka, Á.; Pósa, A.; Matusovits, D. Neutrophil Activation in Diabetes Mellitus (DM)? Role of PAD4 Expression in STZ-Induced Diabetic Rat. Preprints2024, 2024020298. https://doi.org/10.20944/preprints202402.0298.v1
APA Style
Kang, H.L., Kupai, K., Valkusz, Z., Szasz, A., Várkonyi, T., Csonka, Á., Pósa, A., & Matusovits, D. (2024). Neutrophil Activation in Diabetes Mellitus (DM)? Role of PAD4 Expression in STZ-Induced Diabetic Rat. Preprints. https://doi.org/10.20944/preprints202402.0298.v1
Chicago/Turabian Style
Kang, H.L., Anikó Pósa and Danica Matusovits. 2024 "Neutrophil Activation in Diabetes Mellitus (DM)? Role of PAD4 Expression in STZ-Induced Diabetic Rat" Preprints. https://doi.org/10.20944/preprints202402.0298.v1
Abstract
Peptidylarginine deiminases (PADs) have an important role in the pathogenesis of generating citrullinated proteins which is one type of protein posttranslation modifications (PTMs). Additionally, peptidylarginine deiminases 4 (PAD4) is a component of neutrophil extracellular traps (NET) that leads to autoimmune disease during NETosis. Our aim was to check the PAD4 enzyme expression at level of gene and protein expression in streptozotocin (STZ) induced diabetic rat pancreas and checking the link between PAD4 and inflammatory cytokines. Wistar rats were divided into two groups: control group and diabetic STZ group. STZ was administered by intraperitoneally (60 mg/kg/ body weight). Rats were caged for six weeks and then terminated. Pancreatic tissue was harvested for measurement of PAD4 protein expression, PAD4 mRNA, citrulli-nated histone-3 protein (CITH3), cytokines and Ca2+ content. All measurements in diabetic rats were significantly in-creased compared to non-diabetic rats (p
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
