Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Assessment of Cervicovaginal Smear and HPV DNA Cotest for Cervical Cancer Screening: Implications for Diagnosis and Follow-Up Strategies

Version 1 : Received: 30 January 2024 / Approved: 31 January 2024 / Online: 31 January 2024 (12:56:21 CET)

How to cite: Kaya Terzi, N.; Yulek, O. Assessment of Cervicovaginal Smear and HPV DNA Cotest for Cervical Cancer Screening: Implications for Diagnosis and Follow-Up Strategies. Preprints 2024, 2024012187. https://doi.org/10.20944/preprints202401.2187.v1 Kaya Terzi, N.; Yulek, O. Assessment of Cervicovaginal Smear and HPV DNA Cotest for Cervical Cancer Screening: Implications for Diagnosis and Follow-Up Strategies. Preprints 2024, 2024012187. https://doi.org/10.20944/preprints202401.2187.v1

Abstract

OBJECTIVE: Cervical cancer is a major cause of cancer-related mortality, necessitating effective screening and diagnostic methods. This study aimed to assess the performance of cervicovaginal smear (CVS) and Human papilloma virus (HPV)-DNA cotest. STUDY DESIGN: The pathology results of 225 female patients who underwent HPV-DNA testing with CVS between 2014 and 2022 and were subsequently diagnosed by colposcopic cervical biopsy or second CVS were retrospectively analyzed. RESULTS: CVS samples showed Atypical Squamous Cells of Undetermined Significance (ASCUS), Low-grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL) and cervical cancer. Concordance between the first and second diagnoses demonstrated moderate agreement for LSIL. ASCUS cases exhibited a significant correlation with HPV-DNA positivity and higher-grade cervical lesions. In biopsy, sensitivity and specificity for CIN 2 were 67.8% and 67.9%, respectively, while for CIN 3, they were 81.8% and 58.5%. HPV testing showed significant correlation with histopathologic results. In women over 40 years, more intraepithelial lesions were diagnosed compared to younger women (p<0.005). The conventional smear technique proved reliable in detecting high-grade lesions. CONCLUSION: Despite the limitations of our study, our results emphasize the value of HPV-DNA testing to avoid unnecessary interventions and to establish appropriate follow-up strategies.

Keywords

cervical cancer screening; colposcopy; conventional smear; liquid-based cytology; HPV

Subject

Medicine and Pharmacology, Pathology and Pathobiology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.