Inhibition of the Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans

Isabel Ferreira LaRocque-de-Freitas; Elias Barbosa Da Silva-Junior; Leticia Paixão Gemieski; Beatriz da Silva Dias Lima; Aislan de Carvalho Vivarini; Ulisses G. Lopes; Leonardo Freire-de-Lima; Alexandre Morrot; Jose Osvaldo Previato; Lucia Mendonça-Previato; Lucia Helena Pinto-da-Silva; Celio Geraldo Freire-de-Lima; Debora Decote-Ricardo

doi:10.20944/preprints202401.1847.v1

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preprints.org > biology and life sciences > immunology and microbiology > doi: 10.20944/preprints202401.1847.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Inhibition of the Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans

Isabel Ferreira LaRocque-de-Freitas

Elias Barbosa Da Silva-Junior

Leticia Paixão Gemieski

Beatriz da Silva Dias Lima

Aislan de Carvalho Vivarini

Ulisses G. Lopes

Leonardo Freire-de-Lima

Alexandre Morrot

Jose Osvaldo Previato

Lucia Mendonça-Previato

Lucia Helena Pinto-da-Silva

Celio Geraldo Freire-de-Lima

^* ,

Debora Decote-Ricardo

Version 1 : Received: 25 January 2024 / Approved: 25 January 2024 / Online: 26 January 2024 (06:57:56 CET)

How to cite: LaRocque-de-Freitas, I.F.; Da Silva-Junior, E.B.; Gemieski, L.P.; Lima, B.D.S.D.; Vivarini, A.D.C.; Lopes, U.G.; Freire-de-Lima, L.; Morrot, A.; Previato, J.O.; Mendonça-Previato, L.; Pinto-da-Silva, L.H.; Freire-de-Lima, C.G.; Decote-Ricardo, D. Inhibition of the Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans. Preprints 2024, 2024011847. https://doi.org/10.20944/preprints202401.1847.v1 LaRocque-de-Freitas, I.F.; Da Silva-Junior, E.B.; Gemieski, L.P.; Lima, B.D.S.D.; Vivarini, A.D.C.; Lopes, U.G.; Freire-de-Lima, L.; Morrot, A.; Previato, J.O.; Mendonça-Previato, L.; Pinto-da-Silva, L.H.; Freire-de-Lima, C.G.; Decote-Ricardo, D. Inhibition of the Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans. Preprints 2024, 2024011847. https://doi.org/10.20944/preprints202401.1847.v1

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MDPI and ACS Style

LaRocque-de-Freitas, I.F.; Da Silva-Junior, E.B.; Gemieski, L.P.; Lima, B.D.S.D.; Vivarini, A.D.C.; Lopes, U.G.; Freire-de-Lima, L.; Morrot, A.; Previato, J.O.; Mendonça-Previato, L.; Pinto-da-Silva, L.H.; Freire-de-Lima, C.G.; Decote-Ricardo, D. Inhibition of the Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans. Preprints 2024, 2024011847. https://doi.org/10.20944/preprints202401.1847.v1

APA Style

LaRocque-de-Freitas, I.F., Da Silva-Junior, E.B., Gemieski, L.P., Lima, B.D.S.D., Vivarini, A.D.C., Lopes, U.G., Freire-de-Lima, L., Morrot, A., Previato, J.O., Mendonça-Previato, L., Pinto-da-Silva, L.H., Freire-de-Lima, C.G., & Decote-Ricardo, D. (2024). Inhibition of the Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from <em>Cryptococcus neoformans</em>. Preprints. https://doi.org/10.20944/preprints202401.1847.v1

Chicago/Turabian Style

LaRocque-de-Freitas, I.F., Celio Geraldo Freire-de-Lima and Debora Decote-Ricardo. 2024 "Inhibition of the Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from <em>Cryptococcus neoformans</em>" Preprints. https://doi.org/10.20944/preprints202401.1847.v1

Abstract

Cryptococcus neoformans is a fungus that mainly affects the respiratory system and the central nervous system. One of the main virulence factors is the capsule, constituted by the polysaccharides glucuronoxylomannan (GXM) and glucuronoxylomanogalactan (GXMGal). Polysaccharides are immunomodulators. One of the target cell populations for modulation are macrophages, part of the first line of defense, important for innate and adaptive immunity. It has been reported that macrophages can be modulated to act as a "Trojan horse" taking phagocytosed yeasts to strategic sites or having their machinery activation compromised. The scarcity of information on canine cryptococcosis led us to assess whether the purified capsular polysaccharides from C. neoformans would be able to modulate the microbicidal action of macrophages. In the present study, we observed that the capsular polysaccharides, GXM, GXMGal or capsule total did not present toxic effects for the DH82 macrophage cell line. However, it was possible to demonstrate that phagocytic activity was decreased after treatment with polysaccharides. In addition, yeasts recovered from macrophages treated with the polysaccharides, after phagocytosis, could be cultured, showing that their viability was not altered. The polysaccharides led to a reduction in ROS production and the expression of IL-12 and IL-6. We observed that GXMGal inhibits MHC class II expression and GXM reduces ERK phosphorylation. In contrast, GXMGal and GXM were able to increase the PPAR- expression. Furthermore, our data suggests that capsular polysaccharides can reduce the microbicidal activity of canine macrophages DH82.

Keywords

Canine Macrophages; Capsular Polysaccharides; Inhibition; Cryptococcosis

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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