Kim, Y.; Nam, S.; Lim, J.; Jang, M. Autumn Olive (Elaeagnus umbellata Thunb.) Berries Improve Lipid Metabolism and Delay aging in Middle-Aged Caenorhabditis elegans. Int. J. Mol. Sci.2024, 25, 3418.
Kim, Y.; Nam, S.; Lim, J.; Jang, M. Autumn Olive (Elaeagnus umbellata Thunb.) Berries Improve Lipid Metabolism and Delay aging in Middle-Aged Caenorhabditis elegans. Int. J. Mol. Sci. 2024, 25, 3418.
Kim, Y.; Nam, S.; Lim, J.; Jang, M. Autumn Olive (Elaeagnus umbellata Thunb.) Berries Improve Lipid Metabolism and Delay aging in Middle-Aged Caenorhabditis elegans. Int. J. Mol. Sci.2024, 25, 3418.
Kim, Y.; Nam, S.; Lim, J.; Jang, M. Autumn Olive (Elaeagnus umbellata Thunb.) Berries Improve Lipid Metabolism and Delay aging in Middle-Aged Caenorhabditis elegans. Int. J. Mol. Sci. 2024, 25, 3418.
Abstract
This study aimed to evaluate the positive effect of autumn olive berries (AOB) extract on aging delay by improving the lipid metabolism of middle-aged C. elegans who became obese due to high glucose (GLU) diet. As a result of investigating the total phenol content and DPPH radical scavenging ability of freeze- or spray-dried AOB, freeze-dried AOB showed better antioxidant potential. Afterward, through HPLC analysis, it was confirmed that catechin was the main compound in the polyphenols of AOB. Therefore, we used freeze-dried AOB in subsequent in vivo experiments. AOB inhibited lipid accumulation in both the young adult and middle-aged groups in a concentration-dependent manner under both normal and 2% GLU conditions. Additionally, AOB inhibited ROS accumulation in a concentration-dependent manner under normal and 2% GLU conditions in the middle-aged worms. In particular, AOB also increased the number of body bending and egg production in middle-aged worms. To confirm the intervention of genetic factors related to lipid metabolism in the effect of AOB, body lipid accumulation was confirmed using worms deficient in the daf-16, atgl-1, aak-1, and akt-1. Regarding the effect of AOB on reducing lipid accumulation, the impact was nullified in daf-16 deficient worms under the 2% GLU condition and nullified in both daf-16 and deficient worms under fasting conditions. This means that AOB regulates lipogenesis and lipolysis by mediating daf-16 and atgl-1 in middle-aged worms. Our results suggest that AOB contributes to the aging delay effect by improving lipid metabolism in metabolic impaired middle-aged worms.
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