Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Associations of the Single Bovine Embryo Growth Media Metabolome with Successful Pregnancy

Elina Tsopp
* ORCID logo ,
Kalle Kilk
ORCID logo ,
Egon Taalberg
,
Pille Pärn
,
Anni Viljaste-Seera
,
Ants Kavak
,
Ülle Jaakma
Version 1 : Received: 28 December 2023 / Approved: 29 December 2023 / Online: 29 December 2023 (13:09:08 CET)

A peer-reviewed article of this Preprint also exists.

Tsopp, E.; Kilk, K.; Taalberg, E.; Pärn, P.; Viljaste-Seera, A.; Kavak, A.; Jaakma, Ü. Associations of the Single Bovine Embryo Growth Media Metabolome with Successful Pregnancy. Metabolites 2024, 14, 89. Tsopp, E.; Kilk, K.; Taalberg, E.; Pärn, P.; Viljaste-Seera, A.; Kavak, A.; Jaakma, Ü. Associations of the Single Bovine Embryo Growth Media Metabolome with Successful Pregnancy. Metabolites 2024, 14, 89.

Abstract

This study investigated whether metabolomic fingerprints of bovine embryo growth media improve the prediction of successful embryo implantation. In this prospective cohort study, the metabolome from in vitro produced Day 7 blastocysts with successful implantation (n=11), blas-tocysts with failed implantation (n=10), and plain culture media without embryos (n=5) was in-cluded. Samples were analyzed using AbsoluteIDQ® p180 Targeted Metabolomics Kit with LC-MS/MS, and a total of 189 metabolites were analyzed from each sample. Blastocysts that re-sulted in successful embryo implantation had significantly higher levels of methionine sulfoxide (p<0.001), DOPA (p<0.05), spermidine (p<0.001), acetylcarnitine to free carnitine ratio (p<0.05), C2+C3 to free carnitine ratio (p<0.05), and lower levels of threonine (nep<0.001) and phosphati-dylcholine PC ae C30:0 (p<0.001) compared to control media. However, when compared to embryos that failed to implant, only DOPA, spermidine, C2/C0, (C2+C3)/C0 and PC ae C30:0 levels differentiated significantly. In summary, our study identifies a panel of differential metabolites in the culture media of bovine blastocysts that could act as potential biomarkers for the selection of viable blastocysts before embryo transfer.

Keywords

bovine; embryo; metabolomics; in vitro; pregnancy; biomarkers

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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