Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chitosan Nanoparticle Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in SH-SY5Y Cell Line

Fadime Canbolat
* ,
Neslihan Demir
ORCID logo ,
Ozlem Tonguc Yayıntas
ORCID logo ,
Melek Pehlivan
,
Aslı Eldem
,
Tulay Kilicaslan Ayna
,
Mehmet Senel
ORCID logo
Version 1 : Received: 25 December 2023 / Approved: 26 December 2023 / Online: 26 December 2023 (10:12:20 CET)

A peer-reviewed article of this Preprint also exists.

Canbolat, F.; Demir, N.; Yayıntas, O.T.; Pehlivan, M.; Eldem, A.; Ayna, T.K.; Senel, M. Chitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line. Biomedicines 2024, 12, 287. Canbolat, F.; Demir, N.; Yayıntas, O.T.; Pehlivan, M.; Eldem, A.; Ayna, T.K.; Senel, M. Chitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line. Biomedicines 2024, 12, 287.

Abstract

Background: Multiple drug delivery systems obtained by loading nanoparticles (NPs) with different drugs with different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading Quercetin (Que) and valproic acid (VPA) onto chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of single (Que or VPA) and dual-drug (Que and VPA) loaded chitosan NPs, characterization of the NPs, conducting in vitro antioxidant activity studies, and analysis of the cytotoxicity and antioxidant activity of the NPs in SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H2O2-induced cell damage were, respectively, 96 µg/ml of Que-loaded chitosan NP (77.30%, 48 h), 2 µg/ml of VPA-loaded chitosan NP (70.06%, 24 h), 96 µg/ml of blank chitosan NP (68.31%, 48 h), and 2 µg/ml of Que and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm in developing drug-loaded NPs, with uniform and homogeneous distribution of drugs onto NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed.

Keywords

antioxidant effect; chitosan; nanoparticle; oxidative stress; quercetin; valproic acid

Subject

Medicine and Pharmacology, Pharmacology and Toxicology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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