Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Mesenchymal Stem Cell Transplantation has A Regenerative Effect in Ischemic Myocardium: an Experimental Rat Model Evaluated by SPECT-CT Assessment.

Antonella Koutela
* ORCID logo ,
George Loudos
,
Maritina Rouchota
,
Dimitrios Kletsas
ORCID logo ,
Andreas Karameris
,
George Vilaras
,
Despoina Myoteri
,
George C. Zografos
,
Dimitrios Dougenis
ORCID logo ,
Apostolos E. Papalois
Version 1 : Received: 20 December 2023 / Approved: 21 December 2023 / Online: 21 December 2023 (04:04:21 CET)

A peer-reviewed article of this Preprint also exists.

Koutela, A.; Loudos, G.; Rouchota, M.; Kletsas, D.; Karameris, A.; Vilaras, G.; Zografos, G.C.; Myoteri, D.; Dougenis, D.; Papalois, A.E. Mesenchymal Stem Cell Transplantation Has a Regenerative Effect in Ischemic Myocardium: An Experimental Rat Model Evaluated by SPECT-CT Assessment. Diagnostics 2024, 14, 401. Koutela, A.; Loudos, G.; Rouchota, M.; Kletsas, D.; Karameris, A.; Vilaras, G.; Zografos, G.C.; Myoteri, D.; Dougenis, D.; Papalois, A.E. Mesenchymal Stem Cell Transplantation Has a Regenerative Effect in Ischemic Myocardium: An Experimental Rat Model Evaluated by SPECT-CT Assessment. Diagnostics 2024, 14, 401.

Abstract

Abstract : Aims: Regeneration of myocardial tissue and improvement of myocardial activity after ischemia is one of the major fields of interest in cardiovascular research. We have developed a novel experimental rat model, and used it to examine the effect of mesenchymal stem cell transplantation (MSCT) on myocardial ischemia evaluated by single photon emission computed tomography (SPECT/CT) and immunohistochemistry. Methods and results: Forty adult female Wistar rats underwent open thoracotomy with (n=30) or without (n=10) surgical ligation of the left anterior descending coronary artery (LAD), to create myocardial ischemia, which was confirmed with ECG. Myocardial viability was evaluated via SPECT/CT at 7 days before as well as at 7 and 14 days post-surgery. At day 0, 15 animals received homologous stem cells injected at the ischemic myocardium area. SPECT/CT evaluation confirmed the reduction of functional myocardial cells of the left ventricle one week post-infarction. Regeneration of the ischemic myocardium fifteen days post infarction was recorded, only in animals submitted to stem cell transplantation. Findings were also confirmed by histology and immunohistochemical analysis, with significant higher expression of GATA4 and Nkx2,5. Conclusion: The positive effect of mesenchymal stem cells transplantation in the ischemic myocardium was recorded. The application of SPECT- CT qualitative and quantitative evaluation of myocardial function may lead to a new approach in experimentation with an anticipated significant impact in the ongoing cardiovascular laboratory research. In clinical perspective, MSCT may be beneficial when accompanied with myocardial revascularization procedures.

Keywords

myocardial regeneration; stem cells transplantation; experimental; immunohistochemistry; SPECT imaging

Subject

Medicine and Pharmacology, Cardiac and Cardiovascular Systems

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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