Osire, T.; Wang, Y.; Lu, G.; Popova, L.; Burtseva, O.; Parshina, E.Y.; Sokolova, O.S. Functionalized Bacteriophages Silk Fibroin-Based Films with Antimicrobial Potential. Preprints2023, 2023121238. https://doi.org/10.20944/preprints202312.1238.v1
APA Style
Osire, T., Wang, Y., Lu, G., Popova, L., Burtseva, O., Parshina, E.Y., & Sokolova, O.S. (2023). Functionalized Bacteriophages Silk Fibroin-Based Films with Antimicrobial Potential. Preprints. https://doi.org/10.20944/preprints202312.1238.v1
Chicago/Turabian Style
Osire, T., Evgeniya Yu. Parshina and Olga S Sokolova. 2023 "Functionalized Bacteriophages Silk Fibroin-Based Films with Antimicrobial Potential" Preprints. https://doi.org/10.20944/preprints202312.1238.v1
Abstract
Scaffolds used for tissue/ bone defect repair must possess versatile properties suitable for each specific application. The issue of antibiotic resistance poses a significant challenge in bone tissue engineering especially when dealing with microbial infections. The most effective approach to address this challenge is to promote the integration of tissue before bacteria can adhere to it, hence preventing the proliferation of certain bacterial strains on the implant. The utilization of 3D printing and or the use of various composite polymers in scaffolds to create antibacterial scaffolds that possess both adequate mechanical strength and exceptional biocompatibility is an attractive approach for addressing the challenges associated with microbial infections in tissue. Herein, a new phage functionalized scaffold was created through surface charge modification of the composite scaffold with polyethyleimine (PEI). Afterwards, the PEI polymerized Scaffolds were incubated with phage lysate for approximately 2h prior to rinsing with ethanol. The morphological and physiochemical properties of formed scaffold were assessed through Raman spectrophotometry while the antibacterial assays were done through growth inhibition zones/ cell viability assays. The polymerized phage composite SF20_PEI.AR9 possessed the highest antimicrobial effect with clear inhibition zones of about 78.30 mm. This could be attributed to the lytic effect of phages on the bacterial cells. Moreover, the enhanced phage effect on the scaffolds was also associated to the improved surface charge on the scaffold, 78,3±7,6 mm which ultimately promoted phage scaffold interface integration hence the high antibacterial activity. The PEI polymerization approach could serve as a model for future development of phage scaffolds.
Keywords
bacteriophage AR9; Bacillis subtilis; silk fibroin scaffolds; Raman spectroscopy; SEM
Subject
Medicine and Pharmacology, Complementary and Alternative Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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