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The Conventional and Breakthrough Tool for the Study of L-glutamate Transporters
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Version 1
: Received: 4 December 2023 / Approved: 4 December 2023 / Online: 4 December 2023 (10:03:27 CET)
A peer-reviewed article of this Preprint also exists.
Takahashi, K.; Sato, K. The Conventional and Breakthrough Tool for the Study of L-Glutamate Transporters. Membranes 2024, 14, 77. Takahashi, K.; Sato, K. The Conventional and Breakthrough Tool for the Study of L-Glutamate Transporters. Membranes 2024, 14, 77.
Abstract
In our recent report, we clarified the direct interaction between excitatory amino acid transporter (EAAT) 1/2 and polyunsaturated fatty acids (PUFAs) by applying electrophysiological and molecular biological techniques to Xenopus oocytes. Xenopus oocytes have a long history of use in the scientific field, but they are still attractive experimental systems for neuropharmacological studies. We will therefore summarize the pharmacological significance, advantages, (especially in the study of EAAT2), and experimental techniques that can be applied to Xenopus oocytes; our new findings concerning L-glutamate (L-Glu) transporters and PUFAs; and the significant outcomes of our data. The data obtained from electrophysiological and molecular biological studies of Xenopus oocytes have provided us with further important questions, such as whether or not some PUFAs can modulate EAATs as allosteric modulators and to what extent docosahexaenoic acid (DHA) affects neurotransmission and thereby affects brain functions. By combining Xenopus oocyte experiments and more translational approaches, we can clarify the functions of proteins that are difficult to examine using cultured cells and the physiological roles of these proteins in brain functions. These approaches can lead to the development of the new central nervous system (CNS) drugs targeting molecules and investigations of their effectiveness.
Keywords
Xenopus oocyte; glutamate transporter; EAAT2; two-electrode voltage clamp; overexpression; excitotoxicity
Subject
Medicine and Pharmacology, Neuroscience and Neurology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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