Larach, A.; Vega-Celedón, P.; Castillo-Novales, D.; Tapia, L.; Cuneo, I.; Cádiz, F.; Seeger, M.; Besoain, X. Diplodia seriata Biocontrol Is Altered via Temperature and the Control of Bacteria. Microorganisms2024, 12, 350.
Larach, A.; Vega-Celedón, P.; Castillo-Novales, D.; Tapia, L.; Cuneo, I.; Cádiz, F.; Seeger, M.; Besoain, X. Diplodia seriata Biocontrol Is Altered via Temperature and the Control of Bacteria. Microorganisms 2024, 12, 350.
Larach, A.; Vega-Celedón, P.; Castillo-Novales, D.; Tapia, L.; Cuneo, I.; Cádiz, F.; Seeger, M.; Besoain, X. Diplodia seriata Biocontrol Is Altered via Temperature and the Control of Bacteria. Microorganisms2024, 12, 350.
Larach, A.; Vega-Celedón, P.; Castillo-Novales, D.; Tapia, L.; Cuneo, I.; Cádiz, F.; Seeger, M.; Besoain, X. Diplodia seriata Biocontrol Is Altered via Temperature and the Control of Bacteria. Microorganisms 2024, 12, 350.
Abstract
Grapevine trunk diseases (GTDs) attack the vine's wood, devastating vineyards worldwide. Chile is the world's fourth largest wine exporter, and Cabernet Sauvignon leads the red wine varieties. Botryosphaeria dieback is an important GTDs and Diplodia seriata is one of the main pathogenic species. Biocontrol studies of these pathogens are commonly carried out at different incubation times but at a single temperature. This study aimed to evaluate the biocontrol effect of native Chilean bacteria against D. seriata isolates at different temperatures. We analyzed the biocontrol effects of Pseudomonas sp. GcR15a, Pseudomonas sp. AMCR2b and Rhodococcus sp. PU4, with three D. seriata isolates (PUCV 2120, PUCV 2142 and PUCV 2183) at 8, 22 and 35°C. Agar plug diffusion and double plate methods were used to evaluate the effect of diffusible and volatile organic compounds (DOC and VOC), and an in vivo test was performed with Cabernet Sauvignon cuttings. Pseudomonas strains showed the most significant inhibition in the DOC assay, and a demelanizing activity was observed. Rhodococcus sp. PU4 showed VOC biocontrol potential at high temperatures (35°C). In vivo, a biocontrol effect was observed against two D. seriata isolates, while the PUCV 2142 isolate proved to be more resistant to being controlled. The biocontrol ability at low temperatures is promising for effective control in the field, where infections occur primarily in winter.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
*
