Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genome-Wide Identification, Expression and Interaction Analyses of PP2C Family Genes in Chenopodium quinoa

Dongdong Yang
,
Xia Zhang
ORCID logo ,
Meng Cao
,
Lu Yin
,
Aihong Gao
,
Kexin An
,
Songmei Gao
,
Shanli Guo
* ,
Haibo Yin
* ORCID logo
Version 1 : Received: 29 November 2023 / Approved: 29 November 2023 / Online: 29 November 2023 (11:03:10 CET)

A peer-reviewed article of this Preprint also exists.

Yang, D.; Zhang, X.; Cao, M.; Yin, L.; Gao, A.; An, K.; Gao, S.; Guo, S.; Yin, H. Genome-Wide Identification, Expression and Interaction Analyses of PP2C Family Genes in Chenopodium quinoa. Genes 2024, 15, 41. Yang, D.; Zhang, X.; Cao, M.; Yin, L.; Gao, A.; An, K.; Gao, S.; Guo, S.; Yin, H. Genome-Wide Identification, Expression and Interaction Analyses of PP2C Family Genes in Chenopodium quinoa. Genes 2024, 15, 41.

Abstract

Plant protein phosphatase 2Cs (PP2Cs) serve as negative regulators of protein kinase cascades activated in different processes and play important roles in plant development and abiotic-stress-mediated signaling pathways. In this study, a genome-wide study was conducted on the CqPP2C gene family. A total of putative 117 CqPP2C genes were identified. Comprehensive analyses of physical and chemical feature, chromosome localization and subcellular localization were conducted. According to phylogenetic analysis, CqPP2Cs were divided into 13 subfamilies. CqPP2Cs in the same subfamily had similar gene structure, conserved motif and all the CqPP2C proteins had the type 2C phosphatase domains. Gene duplication revealed that segmental duplication was the major driving force for CqPP2Cs expansion and all duplicated CqPP2Cs evolved from purifying selection. The expression of CqPP2Cs in various tissues under different abiotic stresses was analyzed using RNA-seq data. The results revealed that CqPP2C genes were involved in regulating development and stress responses of quinoa. Real-time quantitative reverse transcription PCR (qRT-PCR) analysis of six CqPP2C genes in subfamily A revealed that they were up-regulated or down-regulated under salt and drought treatments. Furthermore, the results of yeast two-hybrid assays revealed that subfamily A CqPP2Cs interacted not only with subclass III CqSnRK2s, but also with subclass II CqSnRK2s. Subfamily A CqPP2Cs could interact with CqSnRK2s in different combinations and intensities to respond to various biological processes and stresses. Overall, our results will be useful for understanding the functions of CqPP2C in regulating ABA signals and responding to abiotic stress.

Keywords

Protein phosphatase 2C (PP2C), Chenopodium quinoa, genome-wide, abiotic stress

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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