PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Comprehensive Histopathological and Immunohistochemical Evaluation of African Swine Fever in Romanian Swine: Insights into Pathogenesis and Diagnostic Strategies
Version 1
: Received: 22 November 2023 / Approved: 23 November 2023 / Online: 23 November 2023 (08:42:00 CET)
How to cite:
Ungur, A.; Nagy, A.-L.; Cătoi, C. Comprehensive Histopathological and Immunohistochemical Evaluation of African Swine Fever in Romanian Swine: Insights into Pathogenesis and Diagnostic Strategies. Preprints2023, 2023111487. https://doi.org/10.20944/preprints202311.1487.v1
Ungur, A.; Nagy, A.-L.; Cătoi, C. Comprehensive Histopathological and Immunohistochemical Evaluation of African Swine Fever in Romanian Swine: Insights into Pathogenesis and Diagnostic Strategies. Preprints 2023, 2023111487. https://doi.org/10.20944/preprints202311.1487.v1
Ungur, A.; Nagy, A.-L.; Cătoi, C. Comprehensive Histopathological and Immunohistochemical Evaluation of African Swine Fever in Romanian Swine: Insights into Pathogenesis and Diagnostic Strategies. Preprints2023, 2023111487. https://doi.org/10.20944/preprints202311.1487.v1
APA Style
Ungur, A., Nagy, A. L., & Cătoi, C. (2023). Comprehensive Histopathological and Immunohistochemical Evaluation of African Swine Fever in Romanian Swine: Insights into Pathogenesis and Diagnostic Strategies. Preprints. https://doi.org/10.20944/preprints202311.1487.v1
Chicago/Turabian Style
Ungur, A., Andras-Laszlo Nagy and Cornel Cătoi. 2023 "Comprehensive Histopathological and Immunohistochemical Evaluation of African Swine Fever in Romanian Swine: Insights into Pathogenesis and Diagnostic Strategies" Preprints. https://doi.org/10.20944/preprints202311.1487.v1
Abstract
African Swine Fever (ASF) poses a significant threat to domestic pig populations worldwide. This study investigates the histopathological alterations in confirmed ASF-positive domestic pigs, focusing on over 100 cases from 2018 to 2021. Biological material from 118 pigs, confirmed ASF-positive via PCR examination, was histopathologically examined. Tissue samples from various organs were processed, embedded, and analyzed using hematoxylin and eosin staining. Immunohistochemistry (IHC) was employed to detect the major capsid protein (p72) of the ASF virus.Histological examination revealed characteristic ASF lesions, including severe hemorrhages, lymphoid depletion, and inflammatory infiltrates. Vascular anomalies leading to hemorrhagic events were widespread. Microthromboses were prevalent in lymph nodes and kidneys. IHC detected p72 in various organs, with tonsils consistently showing the highest viral presence.This comprehensive study underscores the pivotal role of lymph nodes, spleen, and kidneys in ASF pathogenesis, highlighting prominent vascular involvement and hemorrhagic manifestations. The study establishes the efficacy of IHC in detecting ASF virus, even in autolyzed tissue samples. Tonsils emerge as a consistent epicenter of viral presence, shedding light on the intricate pathogenesis of ASFV.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
