Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

The Immunogenicity of a Foot-and-Mouth Disease Virus Serotype O Vaccine in Commercial and Subsistence Cattle Herds in Zambia

Frank Banda
* ORCID logo ,
Anna B Ludi
ORCID logo ,
Ginette Wilsden
,
Clare Browning
,
Lynnfield Mooya
,
Masuzyo Ngoma
,
Geoffrey M Muuka
,
Cornelius Mundia
,
Paul Fandamu
,
Henry L Kangwa
,
David J Paton
,
Donald P King
ORCID logo ,
Melvyn Quan
ORCID logo
Version 1 : Received: 8 November 2023 / Approved: 9 November 2023 / Online: 9 November 2023 (13:35:29 CET)

A peer-reviewed article of this Preprint also exists.

Banda, F.; Ludi, A.B.; Wilsden, G.; Browning, C.; Kangwa, H.L.; Mooya, L.; Ngoma, M.; Muuka, G.M.; Mundia, C.; Fandamu, P.; Paton, D.J.; King, D.P.; Quan, M. The Immunogenicity of a Foot-and-Mouth Disease Virus Serotype O Vaccine in Commercial and Subsistence Cattle Herds in Zambia. Vaccines 2023, 11, 1818. Banda, F.; Ludi, A.B.; Wilsden, G.; Browning, C.; Kangwa, H.L.; Mooya, L.; Ngoma, M.; Muuka, G.M.; Mundia, C.; Fandamu, P.; Paton, D.J.; King, D.P.; Quan, M. The Immunogenicity of a Foot-and-Mouth Disease Virus Serotype O Vaccine in Commercial and Subsistence Cattle Herds in Zambia. Vaccines 2023, 11, 1818.

Abstract

The recent introduction of foot-and-mouth disease virus serotype O (O/EA-2 topotype) in Southern Africa region has changed virus dynamics and the FMD vaccine requirements. This study describes an assessment of the heterologous antibody responses induced by O Manisa vaccine (three PD50/dose) against a representative O/EA-2 virus collected from an FMD outbreak in Zambia. Commercial and subsistence cattle herds were monitored following vaccination, where the benefits of employing a double vaccination schedule were compared to single-dose vaccination. Testing using virus neutralisation showed cattle that received two vaccine doses had a mean reciprocal log virus neutralisation titre of 2.02 (standard error [SE] = 0.16, n = 9) for commercial herds and 1.65 (SE = 0.17, n = 5) for subsistence herds , 56 days after first vaccination (dpv). Significantly lower mean titres were observed for single-dosed commercial herds (0.90, SE = 0.08, n = 9) and subsistence herds (1.15, SE = 0.18, n = 3), 56 dpv. Comparison of these results and those generated by solid-phase competitive ELISA by cohen’s kappa coefficient observed a statistically significant (ρ = 0.598 for 1:10 dilution, and ρ = 0.562 for 1:30 dilution of SPCE % inhibition results, p < 0.001 for both dilutions) positive correlation.

Keywords

foot-and-mouth disease; immunogenicity; vaccine; field evaluation

Subject

Medicine and Pharmacology, Veterinary Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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