Version 1
: Received: 30 October 2023 / Approved: 31 October 2023 / Online: 31 October 2023 (08:37:20 CET)
How to cite:
Chernyshkova, O.; Erofeeva, N.; Meshalkina, D.; Balykina, A.; Gambaryan, S.; Belyakov, M.; Firsov, M. Near-Saturating Light Induces a Rapid Increase in cAMP and Activates PKA in Rod Outer Segments of the Frog Retina. Preprints2023, 2023102039. https://doi.org/10.20944/preprints202310.2039.v1
Chernyshkova, O.; Erofeeva, N.; Meshalkina, D.; Balykina, A.; Gambaryan, S.; Belyakov, M.; Firsov, M. Near-Saturating Light Induces a Rapid Increase in cAMP and Activates PKA in Rod Outer Segments of the Frog Retina. Preprints 2023, 2023102039. https://doi.org/10.20944/preprints202310.2039.v1
Chernyshkova, O.; Erofeeva, N.; Meshalkina, D.; Balykina, A.; Gambaryan, S.; Belyakov, M.; Firsov, M. Near-Saturating Light Induces a Rapid Increase in cAMP and Activates PKA in Rod Outer Segments of the Frog Retina. Preprints2023, 2023102039. https://doi.org/10.20944/preprints202310.2039.v1
APA Style
Chernyshkova, O., Erofeeva, N., Meshalkina, D., Balykina, A., Gambaryan, S., Belyakov, M., & Firsov, M. (2023). Near-Saturating Light Induces a Rapid Increase in cAMP and Activates PKA in Rod Outer Segments of the Frog Retina. Preprints. https://doi.org/10.20944/preprints202310.2039.v1
Chicago/Turabian Style
Chernyshkova, O., Michael Belyakov and Michael Firsov. 2023 "Near-Saturating Light Induces a Rapid Increase in cAMP and Activates PKA in Rod Outer Segments of the Frog Retina" Preprints. https://doi.org/10.20944/preprints202310.2039.v1
Abstract
The phototransduction cascade allows the photoreceptor to avoid saturation and retain the ability to detect light over a very wide range of intensities. The main second messenger of the cascade is cGMP and the main regulatory mechanism is calcium feedback, however some experimental data suggest that cAMP may also be involved in the regulation of the phototransduction cascade. For this to be the case, cAMP would have to change on a time scale of seconds. Currently, there are no data on the dynamics of changes in intracellular cAMP levels on this time scale, largely due to the specificity of the sensory modality of photoreceptors, where it is practically impossible to use conventional experimental approaches based on fluorescence methods. In the present work, we used the method of rapid cryofixation of retinal samples after light stimulation and subsequent isolation of outer segment preparations. Levels of cAMP were measured using highly sensitive metabolomics approaches. PKA activity was also measured in these samples by Western blot. We show that when illuminated with near-saturating but still moderate light, cAMP levels rise transiently within approximately the first second and then return to pre-stimulus levels. The increase in cAMP activates PKA, leading to phosphorylation of PKA-specific substrates in frog retinal outer segments.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
