Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Design and Evaluation of an Attractant Sticky Trap for Surveillance of Household Indoors of Female Populations of Aedes aegypti in Mexico

Version 1 : Received: 27 October 2023 / Approved: 30 October 2023 / Online: 30 October 2023 (15:12:43 CET)

A peer-reviewed article of this Preprint also exists.

Paiz-Moscoso, K.E.; Cisneros-Vázquez, L.A.; Danís-Lozano, R.; Rodríguez-Rojas, J.J.; Rebollar-Téllez, E.A.; Sánchez-Casas, R.M.; Fernández-Salas, I. Design and Evaluation of a Sticky Attractant Trap for Intra-Domiciliary Surveillance of Aedes aegypti Populations in Mexico. Insects 2023, 14, 940. Paiz-Moscoso, K.E.; Cisneros-Vázquez, L.A.; Danís-Lozano, R.; Rodríguez-Rojas, J.J.; Rebollar-Téllez, E.A.; Sánchez-Casas, R.M.; Fernández-Salas, I. Design and Evaluation of a Sticky Attractant Trap for Intra-Domiciliary Surveillance of Aedes aegypti Populations in Mexico. Insects 2023, 14, 940.

Abstract

Surveillance is the systematic data collection, analysis, and interpretation essential to planning and implementing control activities. The lack of success in control and surveillance elsewhere for the Ae. aegypti mosquito demands developing new accessible and effective strategies. This work aimed to develop and evaluate an adhesive and lure trap for household indoor and outdoor surveillance of Ae. aegypti. Based on a bibliographic review, four compounds with significant attraction percentages for Ae. aegypti were considered. Our more effective blend was determined by preliminary bioassays using the high-throughput screening system (HITSS) and 90 × 90 cm mosquito cages. We designed a low-cost, pyramid-shaped, sticky cardboard trap to incorporate the selected blend. Semi-field 2 x 2 m cages and field tests were conducted to evaluate its effectiveness through mosquito capture percentages. In laboratory tests, blend number 2 presented an attraction percentage of 47.5 ± 4.8%; while in semi-field cages, a 4-inch, 110-v powered fan was used to disperse the attractants, then a similar capture percentage of 43.2 ± 4.0 % was recorded. Field evaluation of the at-house indoor environment and comparing it with the golden standard BG-Sentinel trap recorded interesting results, i.e., an average of our prototype 6.0 ± 1.5 trapped mosquitoes versus 10.0 ± 2.6. In most Latin American countries, there is a lack of formal and accessible strategies for monitoring adult populations of Ae. aegypti, therefore, must develop tools that reinforce entomological surveillance methods.

Keywords

Aedes aegypti; sticky trap; lure; entomological surveillance.

Subject

Biology and Life Sciences, Insect Science

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