Version 1
: Received: 5 October 2023 / Approved: 5 October 2023 / Online: 5 October 2023 (11:58:08 CEST)
How to cite:
Remesar, X.; Oliva, L.; Fernández-López, J.-A.; Alemany, M. Diet Carbohydrate, Protein Content and Circulating 17-β Estradiol Are Critical Factors for Energy Partition in the Rat. Preprints2023, 2023100281. https://doi.org/10.20944/preprints202310.0281.v1
Remesar, X.; Oliva, L.; Fernández-López, J.-A.; Alemany, M. Diet Carbohydrate, Protein Content and Circulating 17-β Estradiol Are Critical Factors for Energy Partition in the Rat. Preprints 2023, 2023100281. https://doi.org/10.20944/preprints202310.0281.v1
Remesar, X.; Oliva, L.; Fernández-López, J.-A.; Alemany, M. Diet Carbohydrate, Protein Content and Circulating 17-β Estradiol Are Critical Factors for Energy Partition in the Rat. Preprints2023, 2023100281. https://doi.org/10.20944/preprints202310.0281.v1
APA Style
Remesar, X., Oliva, L., Fernández-López, J. A., & Alemany, M. (2023). Diet Carbohydrate, Protein Content and Circulating 17-β Estradiol Are Critical Factors for Energy Partition in the Rat. Preprints. https://doi.org/10.20944/preprints202310.0281.v1
Chicago/Turabian Style
Remesar, X., José-Antonio Fernández-López and Marià Alemany. 2023 "Diet Carbohydrate, Protein Content and Circulating 17-β Estradiol Are Critical Factors for Energy Partition in the Rat" Preprints. https://doi.org/10.20944/preprints202310.0281.v1
Abstract
The relative proportions between nutrients affects the fate of them for metabolic interconversion, storage and turnover or, essentially for energy, in a process of partition modulated by hormonal and metabolic factors. A basic common chow model was used: ST or standard diet, HF high-fat and HP or high-protein had a common substrate. A CF cafeteria-type diet was added for comparison. Rats (female and male) received the diet for 30d. Nutrient intake and body composition were measured. Plasma glucose, lactate, testosterone (T) and estradiol (E2) were measured. Common groups energy intake, was largely based in carbohydrates CH, yielding 6C units, which were fully oxidized or stored as 2C fragments (fatty acids). Lactate (3C) was partially stored as glycerol of TAG. Accrual of 2C far exceeded about ten-fold that of 3C. Amino acid (AA) catabolism yielded 2C, 3C and 4C-5C fragments, the latter acting as Krebs cycle anaplerotic intermediates (KCAI), that facilitated the oxidation of 2C in detriment of TAG accumulation Lactate levels, were related to lactic dehydrogenase activities in muscle and liver. TAG accrual was maximal in CF, quite differently from HF with similar diet lipid proportion. Maximal rates of lipid storage were found in ST (and CF) groups. Females showed a higher ability to oxidize CH and to store less TAG. T increased N and protein deposition, whilst E2 decreased them favoring AA oxidation and the availability of KCAI. In the rat, an effective energy partition scheme requires that about half of the energy is provided by CH (polysaccharide), and needs enough protein to provide KCAI for a smooth 2C oxidation.
Keywords
Energy partition; Rat; Estradiol; Diets; KCAI (Krebs cycle anaplerotic intermediates); Testosterone; Energy balances.
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
