Sénémaud, J.; Skarbek, C.; Hernandez, B.; Song, R.; Pan, Z.; Lefevre, I.; Elisabetta, B.; Castier, Y.; Nicoletti, A.; Bureau, C.; Caligiuri, G. Camouflaging Endovascular Stents With an Endothelial Coat Using CD31 Domain 1 Mimetic Peptides. Preprints2023, 2023092003. https://doi.org/10.20944/preprints202309.2003.v1
APA Style
Sénémaud, J., Skarbek, C., Hernandez, B., Song, R., Pan, Z., Lefevre, I., Elisabetta, B., Castier, Y., Nicoletti, A., Bureau, C., & Caligiuri, G. (2023). Camouflaging Endovascular Stents With an Endothelial Coat Using CD31 Domain 1 Mimetic Peptides. Preprints. https://doi.org/10.20944/preprints202309.2003.v1
Chicago/Turabian Style
Sénémaud, J., Christophe Bureau and Giuseppina Caligiuri. 2023 "Camouflaging Endovascular Stents With an Endothelial Coat Using CD31 Domain 1 Mimetic Peptides" Preprints. https://doi.org/10.20944/preprints202309.2003.v1
Abstract
Background: Implantation of an endovascular device disrupts the homeostatic CD31:CD31 interactions among quiescent endothelial cells, platelets, and circulating leukocytes. The aim of this study was to mask endovascu-lar stents with peptides imitating CD31, to mimic the device's surface to resemble that of a healthy vascular endothelium.
Methods: Peptide design relied on established trans-homophilic sequences found within domains 1 and 2 of CD31. Subsequently, synthetic peptides were immobilized onto flat CoCr and nitinol discs and their impact on the primary human arterial endothelial cell phenotype was evaluated in vitro. Following this, clinical-grade CoCr and nitinol endovascular devices were coated with one of the peptides that demonstrated the capability to induce a pro-physiologic endothelial phenotype, implanted in the arteries of rabbits, and compared to un-coated controls.
Results: Membrane-distal CD31 domains 1 and 2 peptides exhibited a distinct capability to foster a healthy endothelial phenotype in vitro. By day 7 post-aortic implantation, CoCr stents were evenly covered by whole-some endothelial cells, devoid of thrombo-inflammatory signs, in contrast to both bare metal and drug-eluting stents. At day 60, nitinol-coated flow diverters demonstrated enhanced performance in comparison to the control groups for excluding experimental carotid aneurysms, facilitating the formation of a seamless "neo-arterial" wall.
Conclusion: Membrane-distal CD31 biomimetic peptides effectively camouflage the device surface, prevent-ing local reactions and promoting rapid and seamless endovascular integration.
Medicine and Pharmacology, Cardiac and Cardiovascular Systems
Copyright:
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