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Validation of the Histological Diagnosis of Hydatidiform Moles with p57KIP2 Immunophenotyping at the University Teaching Hospitals of Kigali and Butare (CHUK, CHUB)
Version 1
: Received: 10 September 2023 / Approved: 12 September 2023 / Online: 13 September 2023 (09:39:07 CEST)
How to cite:
Thomas, H.; David, N.; Felix, M.; Annette, U.; Belson, R. Validation of the Histological Diagnosis of Hydatidiform Moles with p57KIP2 Immunophenotyping at the University Teaching Hospitals of Kigali and Butare (CHUK, CHUB). Preprints2023, 2023090866. https://doi.org/10.20944/preprints202309.0866.v1
Thomas, H.; David, N.; Felix, M.; Annette, U.; Belson, R. Validation of the Histological Diagnosis of Hydatidiform Moles with p57KIP2 Immunophenotyping at the University Teaching Hospitals of Kigali and Butare (CHUK, CHUB). Preprints 2023, 2023090866. https://doi.org/10.20944/preprints202309.0866.v1
Thomas, H.; David, N.; Felix, M.; Annette, U.; Belson, R. Validation of the Histological Diagnosis of Hydatidiform Moles with p57KIP2 Immunophenotyping at the University Teaching Hospitals of Kigali and Butare (CHUK, CHUB). Preprints2023, 2023090866. https://doi.org/10.20944/preprints202309.0866.v1
APA Style
Thomas, H., David, N., Felix, M., Annette, U., & Belson, R. (2023). Validation of the Histological Diagnosis of Hydatidiform Moles with p57KIP2 Immunophenotyping at the University Teaching Hospitals of Kigali and Butare (CHUK, CHUB). Preprints. https://doi.org/10.20944/preprints202309.0866.v1
Chicago/Turabian Style
Thomas, H., Uwineza Annette and Rugwizangoga Belson. 2023 "Validation of the Histological Diagnosis of Hydatidiform Moles with p57KIP2 Immunophenotyping at the University Teaching Hospitals of Kigali and Butare (CHUK, CHUB)" Preprints. https://doi.org/10.20944/preprints202309.0866.v1
Abstract
Background: The hydatidiform moles remain prevalent in spectrum of gesta-tional trophoblastic diseases (GTDs). In resource-limited settings like Rwanda, the definitive diagnosis relies upon single of histomophological diagnosis. The histomorphology alone suffers from high interobserver and intra-observer vari-ability with poor diagnostic reproducibility. The present study aimed at deter-mining the role of p57 immunophenotyping in the validation of histomorpholog-ical diagnosis of hydatidiform moles.
Methods: A retrospective cross-sectional study embarked for histological cases collected between January 2017 and June 2020. A review of Hematoxylin &Eosin(H&E) stained slides was performed with subsequent p57 immunohisto-chemical staining.
Results: Recorded were 211 retrospective cases of hydatidiform moles and 96 (45.9%) cases were all subjected to p57 immunohistochemical staining consid-ered as gold standard diagnostic modality in the present study. As result, the sensitivity and specificity of the histomophological diagnosis of complete hyda-tidiform mole were estimated at 62.5% and 57.1% respectively with positive and negative likelihood ratio of 0.145 and of 0.54 respectively. PPV and NPV were 81.8% and 29.3%, respectively. Whereas of 57.1% and 79.2% with PPV and NPV of 42.9% and 83.8% respectively for partial hydatidiform moles. Per the Youden J statistics method, the accuracy estimation of histomophological diagnosis of hydatidiform mole (HM) was 0.196 (CHM) and 0.336(PHM).
Conclusions: This study highlighted a need to integrate p57 immunostaining in routine histopathological diagnosis of hydatidiform moles refining the defini-tive diagnosis.
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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