Version 1
: Received: 8 September 2023 / Approved: 11 September 2023 / Online: 12 September 2023 (04:11:32 CEST)
How to cite:
Zhang, J.; Li, F.; Zhong, H.; Chen, J. Symbiont Diversity in Imidacloprid-Resistant and Imidacloprid-Susceptible Populations of Nilaparvata lugens (Hemiptera: Delphacidae). Preprints2023, 2023090680. https://doi.org/10.20944/preprints202309.0680.v1
Zhang, J.; Li, F.; Zhong, H.; Chen, J. Symbiont Diversity in Imidacloprid-Resistant and Imidacloprid-Susceptible Populations of Nilaparvata lugens (Hemiptera: Delphacidae). Preprints 2023, 2023090680. https://doi.org/10.20944/preprints202309.0680.v1
Zhang, J.; Li, F.; Zhong, H.; Chen, J. Symbiont Diversity in Imidacloprid-Resistant and Imidacloprid-Susceptible Populations of Nilaparvata lugens (Hemiptera: Delphacidae). Preprints2023, 2023090680. https://doi.org/10.20944/preprints202309.0680.v1
APA Style
Zhang, J., Li, F., Zhong, H., & Chen, J. (2023). Symbiont Diversity in Imidacloprid-Resistant and Imidacloprid-Susceptible Populations of Nilaparvata lugens (Hemiptera: Delphacidae). Preprints. https://doi.org/10.20944/preprints202309.0680.v1
Chicago/Turabian Style
Zhang, J., Haiying Zhong and Jianming Chen. 2023 "Symbiont Diversity in Imidacloprid-Resistant and Imidacloprid-Susceptible Populations of Nilaparvata lugens (Hemiptera: Delphacidae)" Preprints. https://doi.org/10.20944/preprints202309.0680.v1
Abstract
Imidacloprid, a neonicotinoid, has proven as efficacious against hemipterans including the brown planthopper, Nilaparvata lugens (Stål). Frequent use, however, has resulted in high levels of resistance to imidacloprid among brown planthopper populations. Endosymbionts of insects are important for host insect growth and development and seem to play a role in resistance to imidacloprid. In this study, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the bacterial and yeast-like-symbiont communities of imidacloprid-resistant and imidacloprid-susceptible brown planthopper populations. The Shannon-Weaver diversity index and the evenness index indicated that there were no differences in the composition of the symbiotic communities of resistant and susceptible with a similarity coefficient of 0.53 for bacterial symbionts and 0.56 for yeast-like symbionts for the two planthopper populations. Sequence comparison analysis showed that the numbers of bacterial species in the imidacloprid-resistant versus the imidacloprid-susceptible populations were not significantly different. The bacterial species in the susceptible population were members of the Enterobacteriaceae and Moraxellaceae, while these in the resistant population were members of the Enterobacteriaceae, Oxalobacteriaceae, Rhodobacteriaceae, and Sphingomonadaceae. The imidacloprid-susceptible population had more yeast-like symbiont species than the resistant population.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
