Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice

Sang-Joon Park
* ORCID logo ,
Min-Jeong Jo
,
Youngjae Ryu
,
Myung-Gi Seo
,
Jae Woo Lee
,
Yoon Hee Kim
,
Yongduk Kim
,
Jin-Kyu Park
ORCID logo
Version 1 : Received: 7 September 2023 / Approved: 8 September 2023 / Online: 11 September 2023 (04:11:02 CEST)

How to cite: Park, S.; Jo, M.; Ryu, Y.; Seo, M.; Lee, J.W.; Kim, Y.H.; Kim, Y.; Park, J. Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice. Preprints 2023, 2023090608. https://doi.org/10.20944/preprints202309.0608.v1 Park, S.; Jo, M.; Ryu, Y.; Seo, M.; Lee, J.W.; Kim, Y.H.; Kim, Y.; Park, J. Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice. Preprints 2023, 2023090608. https://doi.org/10.20944/preprints202309.0608.v1

Abstract

Non-alcoholic steatohepatitis (NASH) is diagnosed by the occurrence of fat buildup, inflammation, and fibrotic changes within the liver. NASH has the capacity to proceed to the advancement of decompensated cirrhosis and carcinoma by inflammation and oxidative stress. Although Agrimonia pilosa has traditionally been used medicinal purposes, its effects on the liver remains incompletely elucidated. In this research, we assessed the therapeutic outcomes of Agrimonia pilosa extract (APE) on free fatty acid (FFA)-treated HepG2 cellular hepatic steatosis and in a choline-deficient, L-amino acid-defined, high-fat diet with 0.1% methionine (CDAHFD)-induced NASH mouse model. In FFA-treated cells, APE inhibited intracellular lipid accumulation, and regulated the mRNA and protein expressions of modulators of fatty acid (FA) synthesis as well as FA oxidation proteins, through down-regulation of the AMP-activated protein kinase/sirtuin 1 signaling pathway. In the CDAHFD-induced NASH mouse model, APE significantly enhanced liver histology and serum levels of liver damage. APE also significantly attenuated hepatic expression of mRNA and proteins related to FA synthesis, inflammation, and fibrosis. Furthermore, APE mitigated the expression levels of oxidative stress and endoplasmic reticulum stress. Overall, these discoveries indicate that Agrimonia pilosa has the potential to treat NASH by regulating hepatic lipid metabolism, alleviating inflammation, and mitigating oxidative stress.

Keywords

non-alcoholic steatohepatitis; Agrimonia pilosa; lipid metabolism; inflammation; oxidative stress; AMPK/SIRT1 signaling pathway

Subject

Medicine and Pharmacology, Complementary and Alternative Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.