Prerpints.org logo
Preprint
Review

A Review in Childhood Acute Myeloid Leukemia: Diagnosis and Novel Treatment

Altmetrics

Downloads

253

Views

68

Comments

0

A peer-reviewed article of this preprint also exists.

This version is not peer-reviewed

Submitted:

06 September 2023

Posted:

06 September 2023

You are already at the latest version

Alerts
Abstract
Acute myeloid leukemia (AML) is the second common hematologic malignancy in children. The incidence of childhood AML is much lower than acute lymphoblastic leukemia (ALL), which makes the childhood AML a rare disease in children. The role of genetic abnormalities in AML classification, management and prognosis prediction is much more important than before. The WHO classification of myeloid neoplasms and the European LeukemiaNet (ELN) classification were both revised in 2022. The application of the new information in childhood AML will be upcoming in the next few years. The frequency of each genetic abnormality in adult and childhood AML is different, therefore, in this review we emphasize in well-known genetic subtypes in childhood AML, including Core-binding factor AML (CBF AML), KMT2Ar (KMT2A/11q23 rearrangement) AML, normal karyotypes AML with somatic mutations, unbalanced cytogenetic abnormalities AML, NUP98 11p15/NUP09 rearrangement AML and acute promyelocytic leukemia (APL). Current risk group classification, management algorithm in childhood AML and novel treatment modalities, such as targeted therapy, immune therapy, chimeric antigen receptor (CAR) T-cell therapy are reviewed. Finally, the indications of hematopoietic stem cell transplantation (HSCT) in AML are discussed.
Keywords: 
Subject: Medicine and Pharmacology  -   Pediatrics, Perinatology and Child Health

1. Introduction

Acute leukemia (AL) stands as the most common form of cancer observed among children. Among childhood leukemias, the majority (around 80%) are acute lymphoblastic leukemia (ALL), while a smaller proportion (15-20%) is diagnosed as acute myeloid leukemia (AML) [1]. AML originates from cancerous stem cell precursors, which typically mature into myeloid cells including white blood cells, red blood cells, and platelets. This results in an excessive production of cancerous myeloid stem cells. While AML is relatively rare, occurring at a frequency of only seven cases per million children annually[2,3], its prognosis tends to be poorer compared to other childhood cancers, often marked by a high rate of relapse [4].
Progress in understanding AML at the genetic and molecular levels has led to a better grasp of patient risk and categorization. As a result, novel treatment approaches have experienced a rapid advance, leading to improved results in the management of childhood AML over the last thirty years. Currently, complete remission rate has exceeded 90%, and 10-year overall survival rate has also surpassed 60% [5]. The challenge with AML lies in its susceptibility to a variety of mutations and epigenetic changes that hinder treatment response and elevate the chances of relapse [6].
Despite the integration of findings from adult AML studies into the pediatric context, disparities between childhood and adult AML still persist. Variations in how treatment regimens are tolerated underscore the need for meticulous consideration and tailoring of treatment strategies for childhood AML [4,7]. In this article, we explore several widely acknowledged subtypes of pediatric AML and address important prognostic indicators. Furthermore, both current and prospective treatment strategies are also discussed in detail.

2. Well known subtypes of childhood AML

2.1. Core-binding factor AML

The largest subgroup within pediatric AML is core binding factor AML (CBF AML), comprising around 25% of cases, typically affecting individuals with a median age of 8-9 years [2]. The prominent mutations in CBF AML include t(8;21)(q22;q22) and inv(16)(p13q22), also recognized as t(16:16)(p13;q22), often denoted as t(8;21) and inv(16) [8]. These genetic anomalies give rise to fusion genes RUNX1-RUNX1T1 and CBFβ-MYH11, respectively, which disrupt the CBF complex (consisting of RUNX1-3 + CBFβ) crucial for hematopoiesis. These fusion genes serve as indicators to assess minimal residual disease (MRD) [9]. Notably, differences exist in clinical presentation, morphology, and cytogenetics between t(8;21) and inv(16) AML [10]. While t(8;21) commonly exhibits a slight increase in leukocyte count and reduced levels of bone marrow primitive cells, leading to thrombocytopenia and anemia, inv(16) tends to affect areas such as the skin, lungs, or central nervous system, manifesting as hepatosplenomegaly, lymphadenopathy, and gingival hyperplasia [10]. However, the CBF fusion gene is essential but often insufficient to trigger leukemogenesis [11]. The second-hit hypothesis proposes that secondary mutations in RAS-KIT and FLT3 are commonly observed molecular alterations in CBF AML [8].The therapeutic approach for CBF AML includes a regimen of high-dose cytarabine, fludarabine, and gemtuzumab ozogamicin (GO), yielding estimated 10-year survival rates ≥75% and notably improving prognosis [12,13]. Overall survival and remission rates are greatly enhanced when the FLAG-GO regimen (fludarabine, cytarabine, granulocyte colony-stimulating factor with GO) is employed according to a clinical trial[14]. Additionally, AML cases with KIT mutations experienced bettered disease survival rates through the incorporation of dasatinib [15]. Combining venetoclax with hypomethylating agents has proven effective against inv(16) cases [13]. Individuals diagnosed with t(8;21)-AML have demonstrated favorable results through treatment regimens that encompass increased doses of etoposide, anthracyclines, and cytarabine during the induction phase. Additionally, cumulative elevated doses of cytarabine and etoposide have also contributed to positive treatment outcomes [16].

2.2. KMT2Ar (KMT2A/11q23 rearrangement)

KMT2A, situated on chromosome 11q23, is a pivotal player in normal hematopoietic development. Mutations within KMT2A constitute 16-20% of pediatric AML cases. Applying the FAB classification, approximately 73% of KMT2ArAML falls under the categories of AML M4 or M5. Importantly, KMT2Ar AML is more common in infants, with a prevalence of 47-55% observed in children aged below 2 years [17]. The prognosis for infant AML featuring KMT2Ar mutations is generally unfavorable [18]. The first subtype of KMT2Ar AML enrolled in the 2016 WHO classification is AML characterized by t(9;11)(p21.3;q23.3) KMT2A-MLLT3[19]. In the 2022 WHO classification, AML with KMT2A rearrangement replaces “AML with t(9;11)(p22;q23); KMT2A-MLLT3”, because over 80 KMT2A fusion partners have been described, with MLLT3, AFDN, ELL, and MLLT10 being most common. The fusion partner identified could provide prognostic information which may have impact on monitoring disease [20]. The t(11;19)(q23;p13) translocation, which includes ELL (19p13.1) or MLLT1 (ENL) (19p13.3) partners, contributes to 8% and 6% of KMT2Ar cases respectively, and carries intermediate prognosis [21]. Another subtype, t(1;11)(q21;q23)/KMT2A-MLLT11(AF1Q), shows promise with a favorable prognosis, yet its rarity prevents conclusive assessment. On the other hand, gene fusions associated with unfavorable prognoses encompass cases with t(10;11)(p11.2;q23)/KMT2A-ABI1,t(10;11)(p12;q23)/KMT2A-AF10,and t(6;11)(q27;q23)/KMT2A-MLLT4, constituting 2%, 16%, and 6% of KMT2Ar instances, respectively[8]. The prognostic significance of most KMT2A fusions is not certain in childhood AML, but t(4;11)(q21;q23.3)/KMT2A-MLLT2,t(6;11)(q27;q23)/KMT2A-MLLT4, t(10;11)(p12;q23)/KMT2A-AF10 and t(10;11)(p11.2;q23)/KMT2A-ABI1 were identified with a poor prognosis in most literatures[22].Genetic elements MLLT3, ELL, MLLT10 (10p12), and ABI1 (10p11.2) are often linked to infant AML instances. In contrast, MLLT4 (6q27) is more commonly found in older children, with a median age of 12 years [9,18,23].Regarding treatment, augmenting conventional chemotherapy with gemtuzumab ozogamicin (GO) and combining it with hematopoietic stem cell transplantation (HSCT) has exhibited enhanced outcomes for KMT2A-r AML [24]. Another treatment scheme involves venetoclax, a B-cell lymphoma-2 (BCL-2) blocker, which reactivates the caspase-dependent apoptosis in AML. Recent studies have pointed out that the concurrent use of venetoclax with I-BET151, sunitinib, or thioridazine significantly decreases cell viability in cases of KMT2Ar AML [25].

2.3. Normal karyotypes

Approximately 22-26% of childhood AML cases exhibit normal karyotypes. These patients are categorized into an intermediate risk group, displaying varying prognoses. Notably, the proportion of AML cases with a normal cytogenetic profile in children (15-30%) is smaller compared to adults (40-47%) [26]. Risk assessment is determined by established prognostically significant mutations and cytogenetic abnormalities like NPM1, FLT3-ITD, and CEBPAdm. It's important to recognize that a significant portion of AML cases categorized as having a normal karyotype through conventional cytogenetic analysis may obscure other molecular mutations and rearrangements of diagnostic importance, including FLT3 internal tandem duplications (FLT3-ITD), mixed-lineage leukemia gene-partial tandem duplications (MLLPTD), or nucleophosmin (NPM1) mutations [27,28].Among these, FLT3-ITD mutations, present in 20-25% of childhood AML cases with an otherwise regular karyotype, stand as a crucial marker for poor prognosis [27,29]. MLL-PTD is another mutation linked to poorer prognosis, leading to lowered overall survival (OS) and event-free survival (EFS) in pediatric AML [9]. Conversely, NPM1 mutations occur in 20-30% of normal karyotypepediatric cases and typically result in a positive outlook [28,30]. Notably, the coexistence of NPM1 mutations with FLT3-ITD is correlated with a positive prognosis, whereas WT1 mutations alongside FLT3-ITD exacerbate the prognosis [9].

2.4. Unbalanced Cytogenetic Abnormalities in Childhood AML

About 40% of childhood AML cases involve unbalanced cytogenetic abnormalities, with notable prognostic implications like monosomy 5, del(5q), and monosomy 7. However, despite their poor prognostic influence, these abnormalities are detected in just 5% of cases [9]. Monosomy 7 and del(7q), prevalent in childhood myelodysplastic syndromes (MDS), encompass 40% of cases in that context. Notably, del(7q) is more commonly related to CBF leukemia, while monosomy 7 is frequently linked to inv(3)(q21q26). The unfavorable prognosis tied to monosomy 7 mainly arises from its heightened resistance to induction therapy, yielding complete remission rates of 71% to 83% [17,31,32]. Meanwhile, 10-15% of MDS patients and 40% of secondary AML patients experiences del(5q). Lenalidomide treatment effectively targets CSNK1A1 for ubiquitin-mediated degradation in isolated del(5q) MDS cases [33].Unbalanced cytogenetic abnormalities, like del(5q), have emerged as secondary mutations in two AML subtypes with pre-existing cryptic abnormalities: t(5;11)(q35;p15)/NUP98-NSD1 and the infrequent t(7;21)(p22;q22)/RUNX1-USP42. This underscores the significance of comprehensive cytogenetic and molecular assessments for such cases [9]. Trisomy 8, affecting roughly 10-14% of pediatric AML cases, can appear as the only structural alteration or alongside other genetic irregularities. This anomaly is more frequent in older children (with a median age of 10.1 years) and is often associated with FLT3-ITD mutations [34].

2.5. NUP98 11p15/NUP09 rearrangement

Nucleoporin 11p15/98Kd (NUP98) rearrangements are infrequent, occurring in approximately 3-5% of childhood AML cases and occasionally in young adults. Several different partners for NUP98 have been identified, with the most prevalent being the nuclear receptor-binding SET domain protein 1 (NSD1) gene (5q35), present in around 75% of pediatric NUP98r patients. Notably, research indicates that using double the standard dosage of daunorubicin, leads to improved survival outcomes in patients with an NPM1 mutation [35]. In terms of molecular aspects, CDK6 emerges as a prominently expressed target directly regulated by NUP98-fusion proteins. Intervening with CDK6 activity leads to cell cycle arrest, myeloid differentiation, and apoptosis both in vitro and in vivo. This prompts exploration of CDK6 suppression as a potential tactic to address NUP98 fusion associated AML [36]. Additionally, the inhibition of Menin-MLL1 (for instance, VTP50469) stands as another potential therapeutic approach. Studies have demonstrated that such inhibitors promote the upregulation of differentiation markers like CD11B and downregulation of proleukemogenic transcription factors in NUP98-HOXA9 and NUP98-JARID1A mouse leukemic cell lines, indicating their promise as treatment options [37].

2.6. Acute promyelocytic leukemia (APL)

Acute promyelocytic leukemia (APL) is an extensively studied variant of AML and is identified by the translocation t(15;17)(q24;q21), which leads to the generation of the PML-RARA rearrangement [38]. APL is a relatively uncommon ailment, responsible for5%–10% of AML cases. Across all age groups, the United States witnesses fewer than 1000 instances of APL annually [39]. The mutated PML-RARA fusion gene prompts the uncontrolled proliferation and accumulation of leukemic white blood cells (WBCs) that remain arrested at the promyelocyte stage, failing to mature or differentiate. The proliferation of these abnormal cells eventually displaces normal blood cell precursors within the bone marrow, resulting in common symptoms like anemia, prolonged bleeding, and recurrent infections. A serious complication of APL is disseminated intravascular coagulation (DIC) [40]. While around 90% of APL cases exhibit the characteristic t(15;17) PML-RARA reciprocal translocation, there are instances of other translocations involving the RARA gene and other genes apart from PML. The presence of PML-RARA can be determined through techniques like RQ-PCR (real-time quantitative polymerase chain reaction), FISH (fluorescence in situ hybridization), and chromosome analysis [41].

3. Current Treatment Regimens and Future Treatments

Present treatment protocols for AML involve utilizing high doses of cytarabine and anthracycline to achieve complete remission. However, this approach comes with significant drawbacks such as a heightened risk of infection and cardiac dysfunction. Furthermore, primary chemotherapy faces substantial resistance across various AML subtypes, including DNMT3A, TET2, IDH1/2 (more prevalent in adults), and UP98, WT1, RUNX1, MLLT10, SPECC1, and KMT2A [42]. AML cases with unfavorable genetic factors, as outlined in the European LeukemiaNet (ELN) classification, consistently demonstrate resistance to standard chemotherapy, which consequently leads to an unfavorable prognosis [43]. Rubnitz J. had proposed a risk classification of childhood AML based on important genetic factors (Table 1) and MRD status in 2017., which practically guide the treatment strategies for pediatric AML management (Figure 1) [5]. The genetic abnormalities with prognostic significance in childhood AML was revised in 2022, in which “the intermediate or unknown”, including t(9;11)(p12;q23)/MLLT3-KMT2A and t(1;22)(p13;q13)/RBM15-MKL1, was eliminated from the list (Table 1). NPM1 mutation with or without FLT3-ITD and CEBPA mutation with or without FLT3-ITD were categorized as favorable group and FLT3-ITD without CEPBA or NPM1 mutation was categorized as unfavorable group in this version [22]. The 2022 ELN risk classification by genetics at initial diagnosis categorized mutated NPM1 with FLT3-ITD as intermediate group [44]. The suggested treatment algorithm for childhood AML was also revised and enrolled a new entity of relapse/refractory AML in 2021 (Figure 2) [22].

3.1. Target Therapies

A. Gemtuzumab ozogamicin
Geznmtuzumab ozogamicin (GO) is a CD33 targeting monoclonal antibody attached to a toxin. Studies indicate that using GO in induction therapy can reduce relapse risk, though upfront use was linked to increased treatment-related mortality [20]. Although not currently considered for treatment decisions [45], genetic factors such as elevated CD33 expression, KMT2A rearrangements, FLT3-ITD abnormalities, and single-nucleotide polymorphisms in ABCB1 could benefit from GO treatment [46,47].
B. CD123 Target Therapy
CD123 (the IL-3 receptor α-chain) present in many AML cases including leukemic stem cells, is a promising target for therapy. While still in early phases, CD123-targeted treatments offer a potential avenue for treatment [4].
C. FLT3 inhibitors
FLT3 inhibitors are a class of molecules that attach to the ATP-binding site on FLT3, resulting in competitive inhibition of kinase activity[48]. These inhibitors come in two classes, type I and type II, both of which target internal tandem duplication (ITD) mutations in FLT3. Type I inhibitors, in addition to being active against tyrosine kinase domain (TKD) mutations, possess a higher binding affinity for FLT3 [49]. While at least 8 FLT3 inhibitors are under development, none have yet received approval for pediatric use [4].Sorafenib was among the first multikinase type II inhibitor to be administered for AML treatment. Studies indicated that when used in synergy with chemotherapy, sorafenib yielded better complete remission (CR) and event-free survival (EFS) results in younger adult AML patients, regardless of FLT3 mutation status. Nonetheless, this combination also raised concerns regarding increased toxicity [50,51]. Sorafenib was proven to be effective and tolerable for children with relapsed AML when administered along chemotherapy in early-phase trials. As a result, sorafenib could be safely integrated into conventional AML chemotherapy, potentially enhancing outcomes for pediatric high allelic ratio (HAR) FLT3/ITD+ AML cases [52]. Moreover, sorafenib maintenance therapy was found to reduce relapse risk and mortality following hematopoietic cell transplantation (HCT) for FLT3-ITD-positive AML [53]. Midostaurin, a first-generation type I inhibitor that also influences KIT, demonstrated a capacity to decrease blast percentages on its own in patients with relapsed/refractory AML, irrespective of FLT3 mutation status [54]. The extensive international RATIFY study illustrated that combining midostaurin with standard induction therapy followed by consolidation using high-dose cytarabine led to longer overall survival (OS) and event-free intervals compared to chemotherapy alone in newly diagnosed AML patients with FLT3 mutations [43,55].
Gilteritinib, a second-generation type I FLT3 inhibitor, exerts inhibition on AXL, a tyrosine kinase that augments FLT3 activation and contributes to FLT3 inhibitor resistance. Although research involving pediatric patients is currently limited, forthcoming trials are going to explore the application of gilteritinib combined with chemotherapy in FLT3-mutated AML, in both relapse and upfront settings [48]. Notably, highly effective FLT3-specific inhibitors such as quizartinib, gilteritinib, and midostaurin are now accessible for clinical application. In the randomized phase 3 RATIFY trial, integrating typical induction therapy with midostaur in led to extended OS and event-free intervals compared to administering only chemotherapy in individuals newly diagnosed with FLT3-mutated AML [55].
D. BCL-2 inhibitors
BCL-2 inhibitors are significant in AML treatment. One such inhibitor, venetoclax, targets the anti-apoptotic protein BCL-2 abundant in AML cells. There exists evidence indicating that the amalgamation of venetoclax and azacytidine might offer a secure and encouraging treatment alternative for pediatric patients[56]. In a phase 1 study involving continuous 28-day cycles, participants were administered venetoclax orally once a day in combination with IV cytarabine. The study's analysis suggests that the combination of venetoclax and chemotherapy exhibited both safety and effectiveness in heavily relapsed or refractory pediatric AML. This implies the need for further exploration of this combination in newly diagnosed high-risk pediatric AML patients [57]. Notably, the study also reported a remarkable complete response (CR) rate of 70% when venetoclax was administered alongside cytarabine, regardless of the use of idarubicin. Preclinical studies further indicate that the combination of venetoclax with FLT3 inhibitors such as midostaurin and gilteritinib could have a synergistic effect in triggering apoptosis in AML cells, potentially opening up new possibilities for therapy [58].
E. AKT/MAPK/STAT inhibitors
The phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway assumes a central role in regulating cell proliferation, growth, and survival under normal physiological circumstances. This pathway, along with other significant signals like RAS-MAPK-ERK and JAK2-STAT5, serves as a downstream response to various tyrosine kinases, such as FLT3 and ABL1. Amplification of the PI3K-AKT-mTOR pathway is evident in a notable proportion, roughly around 60%, of individuals with AML.[59]. In the realm of AML treatment, three JAK inhibitors—namelypacritinib, ruxolitinib, and lestaurtinib have been subject to clinical evaluations for both AML and high-risk myeloproliferative neoplasms (MPNs) [43]. Pacritinib, administered to a small group of patients dealing with relapsed/refractory AML, demonstrated a clinical benefit rate of 43% [60].
F. Menin-KMT2a inhibitors
AML characterized by KMT2A rearrangements (KMT2Ar) exhibits heightened expressions of HOXA9 and MEIS1 genes. These elevated levels arise from the interaction between oncogenic KMT2A fusion proteins and complex-forming counterparts like LEDGF, DOT1L, and menin. Recent efforts have focused on strategies to disrupt this link between KMT2A protein and menin [4]. Notably, the initial phase 1 study of the menin-KMT2A inhibitor SNDX-5613 (Revumenib) as a standalone treatment showcased promising outcomes among patients with relapsed/refractory AML who carried KMT2A rearrangements or NPM1 mutations [61].
G. Anti-CD47 antibody
CD47, a cell surface protein that is widely distributed, has a pivotal role in regulating phagocytosis via innate immune cells like macrophages and dendritic cells. This modulation takes place via the engagement of CD47 with SIRP-alpha, a receptor found on these immune cells. This interaction imparts a suppressive signal that hinders phagocytosis [62]. As such, prognosis may be unfavorable when AML stem cells express CD47 [63]. In terms of treatment, the anti-CD47 antibody magrolimab, when administered along azacitidine, demonstrated a remarkable 75% overall response rate in adults experiencing untreated higher-risk myelodysplastic syndromes (MDS) [64]. An important step in research is the ongoing phase 2 study led by Gilead, which investigates the therapeutic possibilities of magrolimab when combined with different cytotoxic substances for the treatment of myeloid malignancies.

3.2. Chimeric antigen receptor (CAR) T-cell therapy

CAR-T cell therapy faces challenges in AML due to the absence of a unique cancer surface marker. However, two cell markers, CD33 and CD123, are especially common on AML cells, with CD33 detected in 90% of instances, and CD123 found in 75% of AML cases. Notably, only a minimal proportion (<5%) lacks both[65].While CD33 therapies can affect non-cancerous myeloid cells, the absence of CD33 on hematopoietic stem cells, combined with the favorable results seen with GO, provides a rationale for investigating CD33-targeting CAR-Ts. CD123, found excessively in various hematologic malignancies, serves as an attractive target across leukemia types. Yet, its presence on healthy hematopoietic stem cells suggests it might be best utilized as an intermediary step toward SCT. Ongoing studies involving CAR-T therapies that target CD33 and CD123 are being conducted in pediatric cases of relapsed or refractory AML [66,67,68,69]. It's important to note that immunotarget expression in pediatric AML deviates from adult AML. In pediatric AML, CLEC12A and CD33 stand out as preferred generic combined immunotargets, while CD33 and FLT3 serve as immunotargets specific to KMT2A-mutated infant AML [70].

3.3. Checkpoint Inhibitors

Checkpoint inhibitors such aspembrolizumab and nivolumab, which target PD-1, along with ipilimumab targeting CTLA-4, emerge as promising therapy options. Clinical trials are investigating the synergism between the above mentioned PD-1 inhibitors and anti-PD-L1 antibodies including atezolizumab and durvalumab [71,72].

3.4. Epigenic Therapies

A. Hypomethylating agents (HMAs)
Epigenetic therapies encompass hypomethylating agents (HMAs) like decitabine and azacitidine, which inhibit DNA methyltransferases, inducing tumor suppressor expression and apoptosis [73,74]. St. Jude AML16 trial (NCT03164057) evaluates HMAs alongside cytotoxic chemotherapy [75,76].
B. Histone deacetylase (HDAC) inhibitors
Histone deacetylase (HDAC) inhibitors like vorinostat, panobinostat, tricostatin regulate chromatin by removing acetyl groups from histones, impacting gene transcription, and promoting apoptosis. Synergy with standard chemotherapy is evident [77]. Panobinostat combined with hypomethylating agents and chemotherapy is promising [78]. HDAC inhibitors combine safely with agents like DNMT inhibitors in adult studies and show potential in relapsed pediatric AML [79,80].

3.5. TP53 stabilizers

The TP53 protein plays a crucial role as a tumor suppressor by regulating cell division and apoptosis. TP53 mutations exist in around 30-40% of secondary AML cases and less than 10% of normal AML cases [39]. There are small molecules, such as PRIMA-1, that reactivate mutant TP53. PRIMA-1 and its methylated form induce apoptosis in TP53-mutated cancer cells by restoring wild-type conformation and specific DNA binding of mutant p53 [81,82]. A clinical investigation that combined APR-246 and azacitidine resulted in a 71% overall response rate among patients diagnosed with TP53-mutated high-risk MDS or AML [83]. APR-548, an upcoming TP-53 stabilizer, is under examination in a phase 1 clinical trial (NCT04638309) by Aprea Therapeutics.

3.6. Repurposing of Chemotherapy Drugs

A. CPX-351
CPX-351 involves a liposomal encapsulation of cytarabine and daunorubicin, combined in a synergistic 5:1 drug ratio. It is designed to selectively target leukemia cells while sparing normal bone marrow cells [84]. In pediatric cases of relapsed or refractory AML, a phase I/II trial revealed significant results, as 75% achieved complete remission (CR) following a single CPX-351 treatment cycle. Among these, 80% showed no signs of minimal residual disease (MRD) [85].
B. Oxidative Phosphorylation Inhibitors: Atovaquone repurpose
Atovaquone, an anti-infective agent, is typically administered to prevent and to treat pneumonia, particularly pneumocystis jiroveci pneumonia (PJP), a common concern in AML affected children. Atovaquone also hampers oxidative phosphorylation and thereby helps combat AML in patient-derived xenograft mouse models [86,87].

3.7. Treatment for Acute Promyelocytic Leukemia (APL)

ATRA (all-trans retinoic acid) combined with ATO (arsenic trioxide) and induction chemotherapy represents the current treatment approach for APL, resulting in significantly improved outcomes compared to previous years[38]. This combination has led to high cure rates, with an OS of around 95% and EFS of 90% for pediatric APL patients. The treatment induces the differentiation of APL blasts [88]. Prognosis and treatment response in APL are linked to different PML-RARA isoforms, but the evidence from studies is inconsistent, so current guidelines recommend not altering standard therapy based on isoforms [89]. Recent progress in APL therapy has shifted focus towards minimizing unnecessary treatments, improving treatment tolerance, and enhancing quality of life [39].
ATRA and ATO additions to initial APL therapy have cause drelapsed patients to become insensitive to such treatment. For relapse treatment, options include gemtuzumab ozogamicin (GO), a monoclonal antibody-drug conjugate targeting CD33-expressing cells, which has demonstrated effectiveness in achieving molecular remissions in both newly diagnosed and relapsed patients [90].Another approach is to investigate a synthetic retinoid called tamibarotene, which boasts an elevated binding affinity for PML-RARα.While tamibarotene has shown promise in reducing relapses in high-risk adult APL patients [91], its application to pediatric APL is still under study. Notably, gemtuzumab ozogamicin is approved for relapsed APL cases in children aged 2 years and older who express CD33.
Table 2. Summary of the drugs used in acute promyelocytic leukemia (APL) therapy, adapted from [39].
Table 2. Summary of the drugs used in acute promyelocytic leukemia (APL) therapy, adapted from [39].
Drug Mechanism of Action Role in Current Therapy
ATRA Binding and degradation of PML- RARα fusion protein Standard treatment including induction and post-induction
ATO Induces apoptosis of APL cells Standard treatment including induction and post-induction
Tamibarotene Synthetic retinoid with mechanism similar to ATRA Approved for adult high-risk APL but still under investigation in pediatrics
Gemtuzumab Ozogamicin Induces cell death of CD33 expressing cells via anti-CD33 drug conjugate Approved for relapse therapy in children 2 years and older
Anthracyclines Inhibition of DNA synthesis Only used in induction for HR patients

4. Hematopoietic Stem Cell Transplantation (HSCT) Guidelines for AML

4.1. Hematopoietic Stem Cell Transplant (HSCT) Recommendations

HSCT guidelines emphasize recipient characteristics, donor selection, and preparatory regimen. Such transplantation aims to decrease relapse rates, consolidate graft-versus-leukemia (GVL) effects, and minimize graft-versus-host disease. If unfavorable cytogenetic properties are observed or response to induction therapy is limited, HSCT will be commonly pursued in the first complete remission (CR1) to intensify treatment due to previously suboptimal results with chemotherapy alone [92,93]

4.2. Unfavorable-risk Molecular Abnormality

In situations involving FLT3/ITD, it is recommended that patients proceed to HSCT in CR1, as studies suggest this approach improves outcomes. Co-existing favorable risk mutations, such as NPM1, have been found to mitigate the poor prognostic impact of FLT3/ITD [94,95]. This phenomenon has been observed in adult studies and preliminary research in pediatric cases, possibly suggesting the potential benefits of HSCT in CR1 for this subset of FLT3/ITD patients [96].

4.3. Unfavorable-risk Cytogenetic Abnormality

For cases involving high-risk KMT2Ar, t(6;9)(p23;q34) chromosomal rearrangement, monosomies of 7 and 5 (or del 5q), abnormalities of 3q and 12q, complex karyotype, NUP98-NSD1 fusion, t(5;11)(q35;p15.5), and cryptic chromosome 16 inv(16)(p13.3q24.3), the panel advises HSCT in CR1 for patients with minimal residual disease (MRD) at the end of induction (EOI) stage. If primary induction failure (PIF) persists beyond the second EOI, HSCT should be considered after a third chemotherapy cycle, as achieving complete remission (CR) with further chemotherapy becomes unlikely and risks of toxicity increase. However, factors like emerging therapeutic strategies and disease progression should also guide HSCT timing decisions. Children with therapy-related AML (tAML) are prone to relapse and respond poorly to multiple cycles of chemotherapy due to prior exposure. Early HSCT consideration, generally following 1 or 2 chemotherapy cycles to elicit remission, is advised for these cases [97].

5. Conclusions

Treatment of Pediatric AML still poses many challenges. Our understanding of the genetics and molecular characterization of AML has led to a greater understanding of this disease with better patient risk and categorization. Novel treatment approaches with new therapeutics are also in clinical trials and show promising results. We hope that these new therapies can soon lead to less toxic, more effective and targeted treatment therapies that can help pediatric patients especially with resistant or refractory disease.

Author Contributions

Writing and original draft: ST, MEL, PCL; Writing, review and editing: All authors. All authors have read and agreed to the published version of the manuscript.

Funding

The Ministry of Science and Technology, Taiwan (MOST111-2314-B-037 -078) -

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. De Rooij, J.D.E.; Zwaan, C.M.; Van den Heuvel-Eibrink, M. Pediatric AML: From Biology to Clinical Management. Journal of Clinical Medicine 2015, 4, 127–149. [Google Scholar] [CrossRef] [PubMed]
  2. Creutzig, U.; van den Heuvel-Eibrink, M.M.; Gibson, B.; Dworzak, M.N.; Adachi, S.; de Bont, E.; Harbott, J.; Hasle, H.; Johnston, D.; Kinoshita, A.; et al. Diagnosis and management of acute myeloid leukemia in children and adolescents: recommendations from an international expert panel. Blood 2012, 120, 3187–3205. [Google Scholar] [CrossRef] [PubMed]
  3. Zwaan, C.M.; Kolb, E.A.; Reinhardt, D.; Abrahamsson, J.; Adachi, S.; Aplenc, R.; De Bont, E.S.; De Moerloose, B.; Dworzak, M.; Gibson, B.E.; et al. Collaborative Efforts Driving Progress in Pediatric Acute Myeloid Leukemia. J Clin Oncol 2015, 33, 2949–2962. [Google Scholar] [CrossRef] [PubMed]
  4. Conneely, S.E.; Stevens, A.M. Acute Myeloid Leukemia in Children: Emerging Paradigms in Genetics and New Approaches to Therapy. Curr Oncol Rep 2021, 23, 16. [Google Scholar] [CrossRef] [PubMed]
  5. Rubnitz, J.E. Current Management of Childhood Acute Myeloid Leukemia. Paediatr Drugs 2017, 19, 1–10. [Google Scholar] [CrossRef] [PubMed]
  6. Obszański, P.; Kozłowska, A.; Wańcowiat, J.; Twardowska, J.; Lejman, M.; Zawitkowska, J. Molecular-Targeted Therapy of Pediatric Acute Myeloid Leukemia. Molecules 2022, 27. [Google Scholar] [CrossRef]
  7. Bolouri, H.; Farrar, J.E.; Triche, T.; Ries, R.E.; Lim, E.L.; Alonzo, T.A.; Ma, Y.; Moore, R.; Mungall, A.J.; Marra, M.A.; et al. The molecular landscape of pediatric acute myeloid leukemia reveals recurrent structural alterations and age-specific mutational interactions. Nat Med 2018, 24, 103–112. [Google Scholar] [CrossRef]
  8. Quan, X.; Deng, J. Core binding factor acute myeloid leukemia: Advances in the heterogeneity of. Mol Clin Oncol 2020, 13, 95–100. [Google Scholar] [CrossRef]
  9. Quessada, J.; Cuccuini, W.; Saultier, P.; Loosveld, M.; Harrison, C.J.; Lafage-Pochitaloff, M. Cytogenetics of Pediatric Acute Myeloid Leukemia: A Review of the Current Knowledge. Genes (Basel) 2021, 12. [Google Scholar] [CrossRef]
  10. Solh, M.; Yohe, S.; Weisdorf, D.; Ustun, C. Core-binding factor acute myeloid leukemia: Heterogeneity, monitoring, and therapy. Am J Hematol 2014, 89, 1121–1131. [Google Scholar] [CrossRef]
  11. Rau, R.E. Beyond KIT in CBF-AML: chromatin and cohesin. Blood 2016, 127, 2370–2371. [Google Scholar] [CrossRef] [PubMed]
  12. Kantarjian, H.; Kadia, T.; DiNardo, C.; Daver, N.; Borthakur, G.; Jabbour, E.; Garcia-Manero, G.; Konopleva, M.; Ravandi, F. Acute myeloid leukemia: current progress and future directions. Blood Cancer J 2021, 11, 41. [Google Scholar] [CrossRef] [PubMed]
  13. Sinha, C.; Cunningham, L.C.; Liu, P.P. Core Binding Factor Acute Myeloid Leukemia: New Prognostic Categories and Therapeutic Opportunities. Semin Hematol 2015, 52, 215–222. [Google Scholar] [CrossRef]
  14. Surapally, S.; Tenen, D.G.; Pulikkan, J.A. Emerging therapies for inv(16) AML. Blood 2021, 137, 2579–2584. [Google Scholar] [CrossRef] [PubMed]
  15. Marcucci, G.; Geyer, S.; Laumann, K.; Zhao, W.; Bucci, D.; Uy, G.L.; Blum, W.; Eisfeld, A.K.; Pardee, T.S.; Wang, E.S.; et al. Combination of dasatinib with chemotherapy in previously untreated core binding factor acute myeloid leukemia: CALGB 10801. Blood Adv 2020, 4, 696–705. [Google Scholar] [CrossRef]
  16. Klein, K.; Kaspers, G.; Harrison, C.J.; Beverloo, H.B.; Reedijk, A.; Bongers, M.; Cloos, J.; Pession, A.; Reinhardt, D.; Zimmerman, M.; et al. Clinical Impact of Additional Cytogenetic Aberrations, cKIT and RAS Mutations, and Treatment Elements in Pediatric t(8;21)-AML: Results From an International Retrospective Study by the International Berlin-Frankfurt-Münster Study Group. J Clin Oncol 2015, 33, 4247–4258. [Google Scholar] [CrossRef]
  17. Harrison, C.J.; Hills, R.K.; Moorman, A.V.; Grimwade, D.J.; Hann, I.; Webb, D.K.; Wheatley, K.; de Graaf, S.S.; van den Berg, E.; Burnett, A.K.; et al. Cytogenetics of childhood acute myeloid leukemia: United Kingdom Medical Research Council Treatment trials AML 10 and 12. J Clin Oncol 2010, 28, 2674–2681. [Google Scholar] [CrossRef]
  18. Brown, P.A. Neonatal Leukemia. Clin Perinatol 2021, 48, 15–33. [Google Scholar] [CrossRef]
  19. Arber, D.A.; Orazi, A.; Hasserjian, R.; Thiele, J.; Borowitz, M.J.; Le Beau, M.M.; Bloomfield, C.D.; Cazzola, M.; Vardiman, J.W. The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia. Blood 2016, 127, 2391–2405. [Google Scholar] [CrossRef]
  20. Alaggio, R.; Amador, C.; Anagnostopoulos, I.; Attygalle, A.D.; Araujo, I.B.O.; Berti, E.; Bhagat, G.; Borges, A.M.; Boyer, D.; Calaminici, M.; et al. The 5th edition of the World Health Organization Classification of Haematolymphoid Tumours: Lymphoid Neoplasms. Leukemia 2022, 36, 1720–1748. [Google Scholar] [CrossRef]
  21. Issa, G.C.; Zarka, J.; Sasaki, K.; Qiao, W.; Pak, D.; Ning, J.; Short, N.J.; Haddad, F.; Tang, Z.; Patel, K.P.; et al. Predictors of outcomes in adults with acute myeloid leukemia and KMT2A rearrangements. Blood Cancer J 2021, 11, 162. [Google Scholar] [CrossRef] [PubMed]
  22. Rubnitz, J.E.; Kaspers, G.J.L. How I treat pediatric acute myeloid leukemia. Blood 2021, 138, 1009–1018. [Google Scholar] [CrossRef]
  23. Hernández Sánchez, A.; González, T.; Sobas, M.A.; Sträng, E.; Gastone, C.; Abáigar, M.; Valk, P.J.; Villaverde Ramiro, A.; Benner, A.; Metzeler, K.H.; et al. Rearrangements Involving 11q23/KMT2A: Mutational Landscape and Prognostic Implications - Results of the Harmony Alliance AML Database. Blood 2022, 140, 551–554. [Google Scholar] [CrossRef]
  24. Pollard, J.A.; Guest, E.; Alonzo, T.A.; Gerbing, R.B.; Loken, M.R.; Brodersen, L.E.; Kolb, E.A.; Aplenc, R.; Meshinchi, S.; Raimondi, S.C.; et al. Gemtuzumab Ozogamicin Improves Event-Free Survival and Reduces Relapse in Pediatric KMT2A-Rearranged AML: Results From the Phase III Children's Oncology Group Trial AAML0531. J Clin Oncol 2021, 39, 3149–3160. [Google Scholar] [CrossRef] [PubMed]
  25. Tregnago, C.; Benetton, M.; Da Ros, A.; Borella, G.; Longo, G.; Polato, K.; Francescato, S.; Biffi, A.; Pigazzi, M. Novel Compounds Synergize With Venetoclax to Target KMT2A-Rearranged Pediatric Acute Myeloid Leukemia. Front Pharmacol 2021, 12, 820191. [Google Scholar] [CrossRef]
  26. Stark, B.; Jeison, M.; Gabay, L.G.; Mardoukh, J.; Luria, D.; Bar-Am, I.; Avrahami, G.; Kapeliushnik, Y.; Sthoeger, D.; Herzel, G.; et al. Classical and molecular cytogenetic abnormalities and outcome of childhood acute myeloid leukaemia: report from a referral centre in Israel. Br J Haematol 2004, 126, 320–337. [Google Scholar] [CrossRef]
  27. Zwaan, C.M.; Meshinchi, S.; Radich, J.P.; Veerman, A.J.; Huismans, D.R.; Munske, L.; Podleschny, M.; Hählen, K.; Pieters, R.; Zimmermann, M.; et al. FLT3 internal tandem duplication in 234 children with acute myeloid leukemia: prognostic significance and relation to cellular drug resistance. Blood 2003, 102, 2387–2394. [Google Scholar] [CrossRef]
  28. Brown, P.; McIntyre, E.; Rau, R.; Meshinchi, S.; Lacayo, N.; Dahl, G.; Alonzo, T.A.; Chang, M.; Arceci, R.J.; Small, D. The incidence and clinical significance of nucleophosmin mutations in childhood AML. Blood 2007, 110, 979–985. [Google Scholar] [CrossRef]
  29. Shimada, A.; Taki, T.; Tabuchi, K.; Taketani, T.; Hanada, R.; Tawa, A.; Tsuchida, M.; Horibe, K.; Tsukimoto, I.; Hayashi, Y. Tandem duplications of MLL and FLT3 are correlated with poor prognoses in pediatric acute myeloid leukemia: a study of the Japanese childhood AML Cooperative Study Group. Pediatr Blood Cancer 2008, 50, 264–269. [Google Scholar] [CrossRef]
  30. Manola, K.N. Cytogenetics of pediatric acute myeloid leukemia. Eur J Haematol 2009, 83, 391–405. [Google Scholar] [CrossRef]
  31. Olson, T.S.; Dickerson, K.E.; Nakano, T.A.; Wlodarski, M. Monosomy 7 Predisposition Syndromes Overview. In GeneReviews(®), Adam, M.P., Mirzaa, G.M., Pagon, R.A., Wallace, S.E., Bean, L.J.H., Gripp, K.W., Amemiya, A., Eds.; University of Washington, Seattle. Copyright © 1993-2023, University of Washington, Seattle. GeneReviews is a registered trademark of the University of Washington, Seattle. All rights reserved.: Seattle (WA), 1993. [Google Scholar]
  32. Galaverna, F.; Ruggeri, A.; Locatelli, F. Myelodysplastic syndromes in children. Curr Opin Oncol 2018, 30, 402–408. [Google Scholar] [CrossRef] [PubMed]
  33. Hosono, N. Genetic abnormalities and pathophysiology of MDS. Int J Clin Oncol 2019, 24, 885–892. [Google Scholar] [CrossRef] [PubMed]
  34. Laursen, A.C.; Sandahl, J.D.; Kjeldsen, E.; Abrahamsson, J.; Asdahl, P.; Ha, S.Y.; Heldrup, J.; Jahnukainen, K.; Jónsson Ó, G.; Lausen, B.; et al. Trisomy 8 in pediatric acute myeloid leukemia: A NOPHO-AML study. Genes Chromosomes Cancer 2016, 55, 719–726. [Google Scholar] [CrossRef] [PubMed]
  35. Zhao, J.; Liang, J.W.; Xue, H.L.; Shen, S.H.; Chen, J.; Tang, Y.J.; Yu, L.S.; Liang, H.H.; Gu, L.J.; Tang, J.Y.; et al. The genetics and clinical characteristics of children morphologically diagnosed as acute promyelocytic leukemia. Leukemia 2019, 33, 1387–1399. [Google Scholar] [CrossRef] [PubMed]
  36. Schmoellerl, J.; Barbosa, I.A.M.; Eder, T.; Brandstoetter, T.; Schmidt, L.; Maurer, B.; Troester, S.; Pham, H.T.T.; Sagarajit, M.; Ebner, J.; et al. CDK6 is an essential direct target of NUP98 fusion proteins in acute myeloid leukemia. Blood 2020, 136, 387–400. [Google Scholar] [CrossRef] [PubMed]
  37. Heikamp, E.B.; Henrich, J.A.; Perner, F.; Wong, E.M.; Hatton, C.; Wen, Y.; Barwe, S.P.; Gopalakrishnapillai, A.; Xu, H.; Uckelmann, H.J.; et al. The menin-MLL1 interaction is a molecular dependency in NUP98-rearranged AML. Blood 2022, 139, 894–906. [Google Scholar] [CrossRef]
  38. Liquori, A.; Ibañez, M.; Sargas, C.; Sanz, M.; Barragán, E.; Cervera, J. Acute Promyelocytic Leukemia: A Constellation of Molecular Events around a Single. Cancers (Basel) 2020, 12. [Google Scholar] [CrossRef]
  39. Conneely, S.E.; Stevens, A.M. Advances in Pediatric Acute Promyelocytic Leukemia. Children (Basel) 2020, 7. [Google Scholar] [CrossRef]
  40. Ryan, M.M. Acute Promyelocytic Leukemia: A Summary. J Adv Pract Oncol 2018, 9, 178–187. [Google Scholar]
  41. Mrózek, K.; Bloomfield, C.D. Clinical significance of the most common chromosome translocations in adult acute myeloid leukemia. J Natl Cancer Inst Monogr 2008, 52–57. [Google Scholar] [CrossRef]
  42. McNeer, N.A.; Philip, J.; Geiger, H.; Ries, R.E.; Lavallée, V.P.; Walsh, M.; Shah, M.; Arora, K.; Emde, A.K.; Robine, N.; et al. Genetic mechanisms of primary chemotherapy resistance in pediatric acute myeloid leukemia. Leukemia 2019, 33, 1934–1943. [Google Scholar] [CrossRef]
  43. Ikeda, D.; Chi, S.; Uchiyama, S.; Nakamura, H.; Guo, Y.M.; Yamauchi, N.; Yuda, J.; Minami, Y. Molecular Classification and Overcoming Therapy Resistance for Acute Myeloid Leukemia with Adverse Genetic Factors. Int J Mol Sci 2022, 23. [Google Scholar] [CrossRef] [PubMed]
  44. Dohner, H.; Wei, A.H.; Appelbaum, F.R.; Craddock, C.; DiNardo, C.D.; Dombret, H.; Ebert, B.L.; Fenaux, P.; Godley, L.A.; Hasserjian, R.P.; et al. Diagnosis and management of AML in adults: 2022 recommendations from an international expert panel on behalf of the ELN. Blood 2022, 140, 1345–1377. [Google Scholar] [CrossRef] [PubMed]
  45. Rafiee, R.; Chauhan, L.; Alonzo, T.A.; Wang, Y.C.; Elmasry, A.; Loken, M.R.; Pollard, J.; Aplenc, R.; Raimondi, S.; Hirsch, B.A.; et al. ABCB1 SNP predicts outcome in patients with acute myeloid leukemia treated with Gemtuzumab ozogamicin: a report from Children's Oncology Group AAML0531 Trial. Blood Cancer J 2019, 9, 51. [Google Scholar] [CrossRef] [PubMed]
  46. Lambert, J.; Pautas, C.; Terré, C.; Raffoux, E.; Turlure, P.; Caillot, D.; Legrand, O.; Thomas, X.; Gardin, C.; Gogat-Marchant, K.; et al. Gemtuzumab ozogamicin for de novo acute myeloid leukemia: final efficacy and safety updates from the open-label, phase III ALFA-0701 trial. Haematologica 2019, 104, 113–119. [Google Scholar] [CrossRef] [PubMed]
  47. Castaigne, S.; Pautas, C.; Terré, C.; Raffoux, E.; Bordessoule, D.; Bastie, J.N.; Legrand, O.; Thomas, X.; Turlure, P.; Reman, O.; et al. Effect of gemtuzumab ozogamicin on survival of adult patients with de-novo acute myeloid leukaemia (ALFA-0701): a randomised, open-label, phase 3 study. Lancet 2012, 379, 1508–1516. [Google Scholar] [CrossRef]
  48. Zhao, J.; Song, Y.; Liu, D. Gilteritinib: a novel FLT3 inhibitor for acute myeloid leukemia. Biomark Res 2019, 7, 19. [Google Scholar] [CrossRef]
  49. Kiyoi, H.; Kawashima, N.; Ishikawa, Y. FLT3 mutations in acute myeloid leukemia: Therapeutic paradigm beyond inhibitor development. Cancer Sci 2020, 111, 312–322. [Google Scholar] [CrossRef]
  50. Röllig, C.; Serve, H.; Hüttmann, A.; Noppeney, R.; Müller-Tidow, C.; Krug, U.; Baldus, C.D.; Brandts, C.H.; Kunzmann, V.; Einsele, H.; et al. Addition of sorafenib versus placebo to standard therapy in patients aged 60 years or younger with newly diagnosed acute myeloid leukaemia (SORAML): a multicentre, phase 2, randomised controlled trial. Lancet Oncol 2015, 16, 1691–1699. [Google Scholar] [CrossRef]
  51. Ravandi, F.; Arana Yi, C.; Cortes, J.E.; Levis, M.; Faderl, S.; Garcia-Manero, G.; Jabbour, E.; Konopleva, M.; O'Brien, S.; Estrov, Z.; et al. Final report of phase II study of sorafenib, cytarabine and idarubicin for initial therapy in younger patients with acute myeloid leukemia. Leukemia 2014, 28, 1543–1545. [Google Scholar] [CrossRef]
  52. Pollard, J.A.; Alonzo, T.A.; Brown, P.A.; Gerbing, R.B.; Fox, E.; Choi, J.K.; Fisher, B.T.; Hirsch, B.A.; Kahwash, S.; Levine, J.E. Sorafenib in combination with standard chemotherapy for children with high allelic ratio FLT3/ITD+ AML improves event-free survival and reduces relapse risk: a report from the Children's Oncology Group Protocol AAML1031. Blood 2019, 134, 292. [Google Scholar] [CrossRef]
  53. Burchert, A.; Bug, G.; Fritz, L.V.; Finke, J.; Stelljes, M.; Röllig, C.; Wollmer, E.; Wäsch, R.; Bornhäuser, M.; Berg, T.; et al. Sorafenib Maintenance After Allogeneic Hematopoietic Stem Cell Transplantation for Acute Myeloid Leukemia With FLT3-Internal Tandem Duplication Mutation (SORMAIN). J Clin Oncol 2020, 38, 2993–3002. [Google Scholar] [CrossRef] [PubMed]
  54. Fischer, T.; Stone, R.M.; Deangelo, D.J.; Galinsky, I.; Estey, E.; Lanza, C.; Fox, E.; Ehninger, G.; Feldman, E.J.; Schiller, G.J.; et al. Phase IIB trial of oral Midostaurin (PKC412), the FMS-like tyrosine kinase 3 receptor (FLT3) and multi-targeted kinase inhibitor, in patients with acute myeloid leukemia and high-risk myelodysplastic syndrome with either wild-type or mutated FLT3. J Clin Oncol 2010, 28, 4339–4345. [Google Scholar] [CrossRef] [PubMed]
  55. Stone, R.M.; Mandrekar, S.J.; Sanford, B.L.; Laumann, K.; Geyer, S.; Bloomfield, C.D.; Thiede, C.; Prior, T.W.; Döhner, K.; Marcucci, G.; et al. Midostaurin plus Chemotherapy for Acute Myeloid Leukemia with a FLT3 Mutation. N Engl J Med 2017, 377, 454–464. [Google Scholar] [CrossRef] [PubMed]
  56. Winters, A.C.; Maloney, K.W.; Treece, A.L.; Gore, L.; Franklin, A.K. Single-center pediatric experience with venetoclax and azacitidine as treatment for myelodysplastic syndrome and acute myeloid leukemia. Pediatr Blood Cancer 2020, 67, e28398. [Google Scholar] [CrossRef]
  57. Karol, S.E.; Alexander, T.B.; Budhraja, A.; Pounds, S.B.; Canavera, K.; Wang, L.; Wolf, J.; Klco, J.M.; Mead, P.E.; Das Gupta, S.; et al. Venetoclax in combination with cytarabine with or without idarubicin in children with relapsed or refractory acute myeloid leukaemia: a phase 1, dose-escalation study. Lancet Oncol 2020, 21, 551–560. [Google Scholar] [CrossRef]
  58. Ma, J.; Zhao, S.; Qiao, X.; Knight, T.; Edwards, H.; Polin, L.; Kushner, J.; Dzinic, S.H.; White, K.; Wang, G.; et al. Inhibition of Bcl-2 Synergistically Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Preclinical Models of FLT3-Mutated Acute Myeloid Leukemia. Clin Cancer Res 2019, 25, 6815–6826. [Google Scholar] [CrossRef]
  59. Martelli, A.M.; Evangelisti, C.; Chiarini, F.; McCubrey, J.A. The phosphatidylinositol 3-kinase/Akt/mTOR signaling network as a therapeutic target in acute myelogenous leukemia patients. Oncotarget 2010, 1, 89–103. [Google Scholar] [CrossRef]
  60. Verstovsek, S.; Odenike, O.; Singer, J.W.; Granston, T.; Al-Fayoumi, S.; Deeg, H.J. Phase 1/2 study of pacritinib, a next generation JAK2/FLT3 inhibitor, in myelofibrosis or other myeloid malignancies. J Hematol Oncol 2016, 9, 137. [Google Scholar] [CrossRef]
  61. Fareed, A.; Inam, N.; Faraz, F. Breakthrough treatment choice for Acute Myeloid Leukemia in pediatric and adult patients: Revumenib, an oral selective inhibitor of KMTA2Ar. Rare Tumors 2023, 15, 20363613231183785. [Google Scholar] [CrossRef]
  62. Liu, J.; Wang, L.; Zhao, F.; Tseng, S.; Narayanan, C.; Shura, L.; Willingham, S.; Howard, M.; Prohaska, S.; Volkmer, J.; et al. Pre-Clinical Development of a Humanized Anti-CD47 Antibody with Anti-Cancer Therapeutic Potential. PLoS One 2015, 10, e0137345. [Google Scholar] [CrossRef] [PubMed]
  63. Majeti, R.; Chao, M.P.; Alizadeh, A.A.; Pang, W.W.; Jaiswal, S.; Gibbs, K.D.; van Rooijen, N.; Weissman, I.L. CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells. Cell 2009, 138, 286–299. [Google Scholar] [CrossRef] [PubMed]
  64. Sallman, D.A.; Al Malki, M.M.; Asch, A.S.; Wang, E.S.; Jurcic, J.G.; Bradley, T.J.; Flinn, I.W.; Pollyea, D.A.; Kambhampati, S.; Tanaka, T.N.; et al. Magrolimab in Combination With Azacitidine in Patients With Higher-Risk Myelodysplastic Syndromes: Final Results of a Phase Ib Study. J Clin Oncol 2023, 41, 2815–2826. [Google Scholar] [CrossRef]
  65. Ehninger, A.; Kramer, M.; Röllig, C.; Thiede, C.; Bornhäuser, M.; von Bonin, M.; Wermke, M.; Feldmann, A.; Bachmann, M.; Ehninger, G.; et al. Distribution and levels of cell surface expression of CD33 and CD123 in acute myeloid leukemia. Blood Cancer J 2014, 4, e218. [Google Scholar] [CrossRef] [PubMed]
  66. Tabata, R.; Chi, S.; Yuda, J.; Minami, Y. Emerging Immunotherapy for Acute Myeloid Leukemia. Int J Mol Sci 2021, 22. [Google Scholar] [CrossRef]
  67. Albinger, N.; Pfeifer, R.; Nitsche, M.; Mertlitz, S.; Campe, J.; Stein, K.; Kreyenberg, H.; Schubert, R.; Quadflieg, M.; Schneider, D.; et al. Primary CD33-targeting CAR-NK cells for the treatment of acute myeloid leukemia. Blood Cancer J 2022, 12, 61. [Google Scholar] [CrossRef] [PubMed]
  68. Walter, R.B.; Appelbaum, F.R.; Estey, E.H.; Bernstein, I.D. Acute myeloid leukemia stem cells and CD33-targeted immunotherapy. Blood 2012, 119, 6198–6208. [Google Scholar] [CrossRef]
  69. Loff, S.; Dietrich, J.; Meyer, J.E.; Riewaldt, J.; Spehr, J.; von Bonin, M.; Gründer, C.; Swayampakula, M.; Franke, K.; Feldmann, A.; et al. Rapidly Switchable Universal CAR-T Cells for Treatment of CD123-Positive Leukemia. Mol Ther Oncolytics 2020, 17, 408–420. [Google Scholar] [CrossRef]
  70. Willier, S.; Rothämel, P.; Hastreiter, M.; Wilhelm, J.; Stenger, D.; Blaeschke, F.; Rohlfs, M.; Kaeuferle, T.; Schmid, I.; Albert, M.H.; et al. CLEC12A and CD33 coexpression as a preferential target for pediatric AML combinatorial immunotherapy. Blood 2021, 137, 1037–1049. [Google Scholar] [CrossRef]
  71. Giannopoulos, K. Targeting Immune Signaling Checkpoints in Acute Myeloid Leukemia. J Clin Med 2019, 8. [Google Scholar] [CrossRef]
  72. Daver, N.; Garcia-Manero, G.; Basu, S.; Boddu, P.C.; Alfayez, M.; Cortes, J.E.; Konopleva, M.; Ravandi-Kashani, F.; Jabbour, E.; Kadia, T.; et al. Efficacy, Safety, and Biomarkers of Response to Azacitidine and Nivolumab in Relapsed/Refractory Acute Myeloid Leukemia: A Nonrandomized, Open-Label, Phase II Study. Cancer Discov 2019, 9, 370–383. [Google Scholar] [CrossRef] [PubMed]
  73. Thol, F.; Ganser, A. Treatment of Relapsed Acute Myeloid Leukemia. Curr Treat Options Oncol 2020, 21, 66. [Google Scholar] [CrossRef] [PubMed]
  74. Cruijsen, M.; Lübbert, M.; Wijermans, P.; Huls, G. Clinical Results of Hypomethylating Agents in AML Treatment. J Clin Med 2014, 4, 1–17. [Google Scholar] [CrossRef]
  75. DiNardo, C.D.; Pratz, K.; Pullarkat, V.; Jonas, B.A.; Arellano, M.; Becker, P.S.; Frankfurt, O.; Konopleva, M.; Wei, A.H.; Kantarjian, H.M.; et al. Venetoclax combined with decitabine or azacitidine in treatment-naive, elderly patients with acute myeloid leukemia. Blood 2019, 133, 7–17. [Google Scholar] [CrossRef] [PubMed]
  76. DiNardo, C.D.; Jonas, B.A.; Pullarkat, V.; Thirman, M.J.; Garcia, J.S.; Wei, A.H.; Konopleva, M.; Döhner, H.; Letai, A.; Fenaux, P.; et al. Azacitidine and Venetoclax in Previously Untreated Acute Myeloid Leukemia. N Engl J Med 2020, 383, 617–629. [Google Scholar] [CrossRef] [PubMed]
  77. Quintás-Cardama, A.; Santos, F.P.; Garcia-Manero, G. Histone deacetylase inhibitors for the treatment of myelodysplastic syndrome and acute myeloid leukemia. Leukemia 2011, 25, 226–235. [Google Scholar] [CrossRef]
  78. San José-Enériz, E.; Gimenez-Camino, N.; Agirre, X.; Prosper, F. HDAC Inhibitors in Acute Myeloid Leukemia. Cancers (Basel) 2019, 11. [Google Scholar] [CrossRef]
  79. Craddock, C.F.; Houlton, A.E.; Quek, L.S.; Ferguson, P.; Gbandi, E.; Roberts, C.; Metzner, M.; Garcia-Martin, N.; Kennedy, A.; Hamblin, A.; et al. Outcome of Azacitidine Therapy in Acute Myeloid Leukemia Is not Improved by Concurrent Vorinostat Therapy but Is Predicted by a Diagnostic Molecular Signature. Clin Cancer Res 2017, 23, 6430–6440. [Google Scholar] [CrossRef]
  80. DeAngelo, D.J.; Walker, A.R.; Schlenk, R.F.; Sierra, J.; Medeiros, B.C.; Ocio, E.M.; Röllig, C.; Strickland, S.A.; Thol, F.; Valera, S.Z.; et al. Safety and efficacy of oral panobinostat plus chemotherapy in patients aged 65 years or younger with high-risk acute myeloid leukemia. Leuk Res 2019, 85, 106197. [Google Scholar] [CrossRef]
  81. Bykov, V.J.N.; Issaeva, N.; Zache, N.; Shilov, A.; Hultcrantz, M.; Bergman, J.; Selivanova, G.; Wiman, K.G. Reactivation of mutant p53 and induction of apoptosis in human tumor cells by maleimide analogs. J Biol Chem 2017, 292, 19607. [Google Scholar] [CrossRef]
  82. Rangel, L.P.; Ferretti, G.D.S.; Costa, C.L.; Andrade, S.M.M.V.; Carvalho, R.S.; Costa, D.C.F.; Silva, J.L. p53 reactivation with induction of massive apoptosis-1 (PRIMA-1) inhibits amyloid aggregation of mutant p53 in cancer cells. J Biol Chem 2019, 294, 3670–3682. [Google Scholar] [CrossRef] [PubMed]
  83. Sallman, D.A.; DeZern, A.E.; Garcia-Manero, G.; Steensma, D.P.; Roboz, G.J.; Sekeres, M.A.; Cluzeau, T.; Sweet, K.L.; McLemore, A.; McGraw, K.L.; et al. Eprenetapopt (APR-246) and Azacitidine in. J Clin Oncol 2021, 39, 1584–1594. [Google Scholar] [CrossRef] [PubMed]
  84. Lancet, J.E.; Uy, G.L.; Cortes, J.E.; Newell, L.F.; Lin, T.L.; Ritchie, E.K.; Stuart, R.K.; Strickland, S.A.; Hogge, D.; Solomon, S.R.; et al. CPX-351 (cytarabine and daunorubicin) Liposome for Injection Versus Conventional Cytarabine Plus Daunorubicin in Older Patients With Newly Diagnosed Secondary Acute Myeloid Leukemia. J Clin Oncol 2018, 36, 2684–2692. [Google Scholar] [CrossRef] [PubMed]
  85. Cooper, T.M.; Absalon, M.J.; Alonzo, T.A.; Gerbing, R.B.; Leger, K.J.; Hirsch, B.A.; Pollard, J.; Razzouk, B.I.; Aplenc, R.; Kolb, E.A. Phase I/II Study of CPX-351 Followed by Fludarabine, Cytarabine, and Granulocyte-Colony Stimulating Factor for Children With Relapsed Acute Myeloid Leukemia: A Report From the Children's Oncology Group. J Clin Oncol 2020, 38, 2170–2177. [Google Scholar] [CrossRef]
  86. Stevens, A.M.; Schafer, E.S.; Li, M.; Terrell, M.; Rashid, R.; Paek, H.; Bernhardt, M.B.; Weisnicht, A.; Smith, W.T.; Keogh, N.J.; et al. Repurposing Atovaquone as a Therapeutic against Acute Myeloid Leukemia (AML): Combination with Conventional Chemotherapy Is Feasible and Well Tolerated. Cancers (Basel) 2023, 15. [Google Scholar] [CrossRef] [PubMed]
  87. Stevens, A.M.; Xiang, M.; Heppler, L.N.; Tošić, I.; Jiang, K.; Munoz, J.O.; Gaikwad, A.S.; Horton, T.M.; Long, X.; Narayanan, P.; et al. Atovaquone is active against AML by upregulating the integrated stress pathway and suppressing oxidative phosphorylation. Blood Adv 2019, 3, 4215–4227. [Google Scholar] [CrossRef]
  88. Taga, T.; Tomizawa, D.; Takahashi, H.; Adachi, S. Acute myeloid leukemia in children: Current status and future directions. Pediatr Int 2016, 58, 71–80. [Google Scholar] [CrossRef]
  89. Sanz, M.A.; Fenaux, P.; Tallman, M.S.; Estey, E.H.; Löwenberg, B.; Naoe, T.; Lengfelder, E.; Döhner, H.; Burnett, A.K.; Chen, S.J.; et al. Management of acute promyelocytic leukemia: updated recommendations from an expert panel of the European LeukemiaNet. Blood 2019, 133, 1630–1643. [Google Scholar] [CrossRef]
  90. Lo Coco, F.; Ammatuna, E.; Noguera, N. Treatment of acute promyelocytic leukemia with gemtuzumab ozogamicin. Clin Adv Hematol Oncol 2006, 4, 57–62. [Google Scholar]
  91. Martino, O.D.; Welch, J.S. Retinoic Acid Receptors in Acute Myeloid Leukemia Therapy. Cancers (Basel) 2019, 11. [Google Scholar] [CrossRef]
  92. Elgarten, C.W.; Aplenc, R. Pediatric acute myeloid leukemia: updates on biology, risk stratification, and therapy. Curr Opin Pediatr 2020, 32, 57–66. [Google Scholar] [CrossRef]
  93. Tarlock, K.; Sulis, M.L.; Chewning, J.H.; Pollard, J.A.; Cooper, T.; Gamis, A.; Shenoy, S.; Kutny, M.; Horan, J.; Meshinchi, S.; et al. Hematopoietic Cell Transplantation in the Treatment of Pediatric Acute Myelogenous Leukemia and Myelodysplastic Syndromes: Guidelines from the American Society of Transplantation and Cellular Therapy. Transplant Cell Ther 2022, 28, 530–545. [Google Scholar] [CrossRef]
  94. Gale, R.E.; Green, C.; Allen, C.; Mead, A.J.; Burnett, A.K.; Hills, R.K.; Linch, D.C.; Party, o.b.o.t.M.R.C.A.L.W. The impact of FLT3 internal tandem duplication mutant level, number, size, and interaction with NPM1 mutations in a large cohort of young adult patients with acute myeloid leukemia. Blood 2008, 111, 2776–2784. [Google Scholar] [CrossRef]
  95. Shouval, R.; Labopin, M.; Bomze, D.; Baerlocher, G.M.; Capria, S.; Blaise, D.; Hänel, M.; Forcade, E.; Huynh, A.; Saccardi, R.; et al. Risk stratification using FLT3 and NPM1 in acute myeloid leukemia patients autografted in first complete remission. Bone Marrow Transplantation 2020, 55, 2244–2253. [Google Scholar] [CrossRef]
  96. Tarlock, K.; Alonzo, T.A.; Gerbing, R.B.; Ries, R.E.; Gibson, B.; Niktoreh, N.; Noort, S.; Van den Heuvel-Eibrink, M.M.; Zwaan, C.M.; Meshinchi, S. Distinct Co-Occurring Mutational Profiles in Acute Myeloid Leukemia Confers Prognostic Significance in Children and Young Adults with FLT3/ITD Mutations. Blood 2018, 132, 443–443. [Google Scholar] [CrossRef]
  97. Kobos, R.; Steinherz, P.G.; Kernan, N.A.; Prockop, S.E.; Scaradavou, A.; Small, T.N.; Shukla, N.; Khalaf, R.; O’Reilly, R.J.; Boulad, F. Allogeneic Hematopoietic Stem Cell Transplantation for Pediatric Patients with Treatment-Related Myelodysplastic Syndrome or Acute Myelogenous Leukemia. Biology of Blood and Marrow Transplantation 2012, 18, 473–480. [Google Scholar] [CrossRef]
Preprints 84433 g001
Figure 1. Recommended risk classification based on genetic alterations using prognostically important genetic abnormalities in childhood AML and MRD (minimal residual disease), adapted from [5].
Figure 1. Recommended risk classification based on genetic alterations using prognostically important genetic abnormalities in childhood AML and MRD (minimal residual disease), adapted from [5].
Preprints 84433 g001
Preprints 84433 g002
Figure 2. Recommended Treatment of Childhood AML [22]. Reprinted from Blood, Vol 138 Issue 12 Pages 1009-1018, Jeffrey E. Rubnitz,Gertjan J.L. Kaspers, How I treat pediatric acute myeloid leukemia, Visual abstact, 2021, with permission from Elsevier.
Figure 2. Recommended Treatment of Childhood AML [22]. Reprinted from Blood, Vol 138 Issue 12 Pages 1009-1018, Jeffrey E. Rubnitz,Gertjan J.L. Kaspers, How I treat pediatric acute myeloid leukemia, Visual abstact, 2021, with permission from Elsevier.
Preprints 84433 g002
Table 1. Prognostically important genetic abnormalities in pediatric acute myeloid leukemia proposed in 2017 and 2021 [5,22].
Table 1. Prognostically important genetic abnormalities in pediatric acute myeloid leukemia proposed in 2017 and 2021 [5,22].
2017 2021
Favorable t(8;21)(q22;q22)/RUNX1-RUNX1T1 t(8;21 )(q22;q22)/RUNX1-RUNX1T1
inv(16)(p13.1;q22)/CBFb-MYH11 inv(16)(p13.1;q22)/t(16;16)(p13.1;q22)/CBFB-MYH11
t(16;16)(p13.1;q22)/CBFb-MYH11
Mutated NPM1 without FLT3-ITD NPM1 mutation with or without FLT3-ITD
Biallelic mutations of CEBPA CEBPA mutation with or without FLT3-ITD
t(1;11)(q21;q23)/MLLT11-KMT2A
Unfavorable t(6;11)(q27;q23)/MLLT4-KMT2A inv(16)(p13.3q24.3)/CBFA2T3-GLIS2
t(10;11)(p12;q23)/MLLT10-KMT2A t(10;11)(p12;q23)/KMT2A-AF10
t(10;11)(p11.2;q23)/ABI1-KMT2A t(10;11)(p11.2;q23)/KMT2A-ABI1
t(6;9)(p23;q34)/DEK-NUP214 t(6;11)(q27;q23)/KMT2A-MLLT4
t(8;16)(p11;p13)/KAT6A-CREBBP t(4;11)(q21;q23.3)/KMT2A-MLLT2
t(16;21)(q24;q22)/RUNX1-CBFA2T3 t(11;12)(p15;p13)/NUP98-KDM5A
t(5;11)(q35;p15.5)/NUP98-NSD1 t(7;11)(p15.4;p15)/NUP98-HOXA9
inv(16)(p13.3q24.3)/CBFA2T3-GLIS2 t(5;11)(q35;p15)/NUP98-NSD1
t(11;15)(p15;q35)/NUP98-KDM5A t(6;9)(p23;q34)/DEK-NUP214
t(3;5)(q25;q34)/NPM1-MLF1 t(8;16)(p11;p13)/KAT6A-CREBBP
FLT3-ITD t(16;21)(q24;q22)/RUNX1-CBFA2T3
Monosomy 7 t(7;12)(q36;p13)/ETV6-HLXB
t(3;21)(26.2;q22)/RUNX1-MECOM
t(16;21)(p11.2;q22.2)/ FUS-ERG
FLT3-ITD without CEPBA or NPM1 mutation
inv(3)(q21.3q26.2)/t(3;3)(q21.3q26.2)/RPN1-MECOM
t(3;5)(q25;q34)/NPM1-MLF1
t(10;11)(p12.3;q14.2)/PICALM-MLLT10
-7, -5, 5q-
Intermediate or unknown t(9;11)(p12;q23)/MLLT3-KMT2A
Other KMT2A fusions
t(1;22)(p13;q13)/RBM15-MKL1
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
Copyright: This open access article is published under a Creative Commons CC BY 4.0 license, which permit the free download, distribution, and reuse, provided that the author and preprint are cited in any reuse.
Prerpints.org logo

Preprints.org is a free preprint server supported by MDPI in Basel, Switzerland.

facebook logotwitter logolinkedin logo
MDPI Initiatives
Important Links
Subscribe

Disclaimer

Terms of Use

Privacy Policy

© 2024 MDPI (Basel, Switzerland) unless otherwise stated