Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Combined Biomarker System Predicts Prognosis in Patients with Metastatic Oral Squamous Cell Carcinoma

Version 1 : Received: 4 September 2023 / Approved: 5 September 2023 / Online: 5 September 2023 (07:54:11 CEST)

A peer-reviewed article of this Preprint also exists.

Khromov, T.; Fischer, L.; Leha, A.; Bremmer, F.; Fischer, A.; Schliephake, H.; Rahat, M.A.; Brockmeyer, P. Combined Biomarker System Predicts Prognosis in Patients with Metastatic Oral Squamous Cell Carcinoma. Cancers 2023, 15, 4924. Khromov, T.; Fischer, L.; Leha, A.; Bremmer, F.; Fischer, A.; Schliephake, H.; Rahat, M.A.; Brockmeyer, P. Combined Biomarker System Predicts Prognosis in Patients with Metastatic Oral Squamous Cell Carcinoma. Cancers 2023, 15, 4924.

Abstract

Background: Metastatic oral squamous cell carcinoma (OSCC) is associated with poor patient prognosis. Metastasis is a complex process involving various proteins, tumor cell alterations including changes caused by the epithelial-to-mesenchymal transition (EMT) process, and interactions with the tumor microenvironment (TME). In this study, we investigate a combined protein marker system consisting of connexin 43 (Cx43), EMMPRIN (CD147), E-cadherin and vimentin during the invasive metastatic process of OSCC and the possibility of using this system for prognosis prediction. Methods: The protein expression profiles of Cx43, EMMPRIN, E-cadherin and vimentin were investigated by immunohistochemistry in tissue samples from 24 OSCC patients. The metastatic process was mapped through different regions of interest (ROI) of adjacent healthy oral mucosa (OM), center of primary OSCC, invasive front (IF), and local cervical lymph node metastases (LNM). Disease-free survival (DFS) and overall survival (OS) were the primary clinical endpoints. Results: Significant changes in the expression profiles of the different marker proteins were detected between the different ROIs (all p values < 0.05). Multivariable Cox regression analysis revealed a significant effect of increased EMMPRIN expression towards IF on DFS (p = 0.019) and OS (p = 0.023). The combined predictive analysis showed a significant predictive value of the marker system for DFS (p = 0.0017) and OS (p = 0.00044). Conclusions: The combined marker system was able to significantly predict patient prognosis. An increase in EMMPRIN expression towards IF showed the strongest effect and could be an interesting new antimetastatic therapy approach.

Keywords

oral squamous cell carcinoma; prognosis; overall survival; disease free survival; connexin 43; EMMPRIN; E-cadherin; vimentin; metastasis; epithelial-to-mesenchymal transition

Subject

Medicine and Pharmacology, Pathology and Pathobiology

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